Load time: 04/27/2011 10:35:25 AM Read time: 04/27/2011 10:35:25 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1509 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 3157467 cps Min counts: 70736 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1384929.00 978083.00 1006899.00 258372.00 638608.00 538257.00 1193622.00 141473.00 1355657.00 310031.00 529127.00 1021469.00 B 1203722.00 2529757.00 620272.00 538333.00 811598.00 294073.00 587805.00 399769.00 742440.00 364258.00 864437.00 683040.00 C 612056.00 296413.00 1556074.00 803134.00 594119.00 431933.00 482128.00 278876.00 567890.00 766387.00 524942.00 642526.00 D 647300.00 482204.00 2906633.00 825863.00 474862.00 197393.00 905578.00 2409820.00 374529.00 372000.00 739796.00 449184.00 E 257269.00 1298063.00 1328553.00 758189.00 995943.00 206294.00 1400811.00 1300593.00 405913.00 475546.00 927908.00 570839.00 F 199485.00 516688.00 1230921.00 780823.00 920014.00 158382.00 1035905.00 1241344.00 288900.00 431990.00 524410.00 253522.00 G 309137.00 594519.00 3157467.00 426017.00 1885479.00 325571.00 618009.00 1991672.00 403726.00 419741.00 390887.00 713567.00 H 142785.00 1886681.00 946985.00 178981.00 1809720.00 247397.00 1021317.00 662288.00 441329.00 730838.00 316384.00 223983.00 Load time: 04/27/2011 10:35:25 AM Read time: 04/27/2011 10:35:25 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1509 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 3157467 cps Min counts: 70736 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1384929.00 978083.00 1006899.00 258372.00 638608.00 538257.00 1193622.00 141473.00 1355657.00 310031.00 529127.00 1021469.00 B 1203722.00 2529757.00 620272.00 538333.00 811598.00 294073.00 587805.00 399769.00 742440.00 364258.00 864437.00 683040.00 C 612056.00 296413.00 1556074.00 803134.00 594119.00 431933.00 482128.00 278876.00 567890.00 766387.00 524942.00 642526.00 D 647300.00 482204.00 2906633.00 825863.00 474862.00 197393.00 905578.00 2409820.00 374529.00 372000.00 739796.00 449184.00 E 257269.00 1298063.00 1328553.00 758189.00 995943.00 206294.00 1400811.00 1300593.00 405913.00 475546.00 927908.00 570839.00 F 199485.00 516688.00 1230921.00 780823.00 920014.00 158382.00 1035905.00 1241344.00 288900.00 431990.00 524410.00 253522.00 G 309137.00 594519.00 3157467.00 426017.00 1885479.00 325571.00 618009.00 1991672.00 403726.00 419741.00 390887.00 713567.00 H 142785.00 1886681.00 946985.00 178981.00 1809720.00 247397.00 1021317.00 662288.00 441329.00 730838.00 316384.00 223983.00 Load time: 04/27/2011 10:38:34 AM Read time: 04/27/2011 10:38:34 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1510 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 22452598 cps Min counts: 884 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22452598.00 10902022.00 11051742.00 40722.00 1768.00 1921.00 B 6139249.00 6602454.00 7660403.00 37565.00 1902.00 1902.00 C 3264369.00 3036214.00 4425109.00 36043.00 1940.00 1902.00 D 1746993.00 2094794.00 2269143.00 38782.00 1844.00 1902.00 E 783600.00 949553.00 1048345.00 39448.00 1863.00 1921.00 F 652493.00 866053.00 867347.00 38535.00 2016.00 1883.00 G 677353.00 304477.00 924218.00 39524.00 2016.00 2092.00 H 507767.00 283384.00 726482.00 37222.00 1978.00 2035.00 Load time: 04/27/2011 10:38:34 AM Read time: 04/27/2011 10:38:34 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1510 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 22452598 cps Min counts: 884 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 22452598.00 10902022.00 11051742.00 40722.00 1768.00 1921.00 B 6139249.00 6602454.00 7660403.00 37565.00 1902.00 1902.00 C 3264369.00 3036214.00 4425109.00 36043.00 1940.00 1902.00 D 1746993.00 2094794.00 2269143.00 38782.00 1844.00 1902.00 E 783600.00 949553.00 1048345.00 39448.00 1863.00 1921.00 F 652493.00 866053.00 867347.00 38535.00 2016.00 1883.00 G 677353.00 304477.00 924218.00 39524.00 2016.00 2092.00 H 507767.00 283384.00 726482.00 37222.00 1978.00 2035.00