##BLOCKS= 10 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 24.5 1650361 2558846 7285444 2008621 1747421 25248449.8125 22187555.8125 12928255.8125 13708347.8125 16724846.8125 -243.1875 363.8125 892229 1551306 779149 611264 2326942 17153167.8125 15822017.8125 9895055.8125 5739051.8125 8361307.8125 -236.1875 16.8125 3242448 1615025 21964 1803356 2902847 14203650.8125 11606804.8125 6085338.8125 4631712.8125 5652816.8125 -388.1875 11.8125 2850205 4779826 25304 724133 2531503 17620849.8125 6108178.8125 4634099.8125 4559292.8125 3675551.8125 129.8125 862.8125 4130714 3734425 4565833 635034 995884 9703147.8125 9297091.8125 2758344.8125 2274876.8125 3066753.8125 539.8125 -202.1875 41697 1292236 7274447 2006880 2772278 3588620.8125 3039388.8125 2520167.8125 1817304.8125 2523068.8125 -292.1875 -85.1875 2825453 2552460 3016317 2415328 379347 5697598.8125 5815836.8125 2978718.8125 3107144.8125 2062815.8125 -431.1875 145.8125 4262126 2359954 4646159 2589300 6456454 872274.8125 3122496.8125 2887606.8125 2561597.8125 1799781.8125 -43.1875 -149.1875 1 2 3 4 5 6 7 8 9 10 11 12 1650361 2558846 7285444 2008621 1747421 25248449.8125 22187555.8125 12928255.8125 13708347.8125 16724846.8125 -243.1875 363.8125 892229 1551306 779149 611264 2326942 17153167.8125 15822017.8125 9895055.8125 5739051.8125 8361307.8125 -236.1875 16.8125 3242448 1615025 21964 1803356 2902847 14203650.8125 11606804.8125 6085338.8125 4631712.8125 5652816.8125 -388.1875 11.8125 2850205 4779826 25304 724133 2531503 17620849.8125 6108178.8125 4634099.8125 4559292.8125 3675551.8125 129.8125 862.8125 4130714 3734425 4565833 635034 995884 9703147.8125 9297091.8125 2758344.8125 2274876.8125 3066753.8125 539.8125 -202.1875 41697 1292236 7274447 2006880 2772278 3588620.8125 3039388.8125 2520167.8125 1817304.8125 2523068.8125 -292.1875 -85.1875 2825453 2552460 3016317 2415328 379347 5697598.8125 5815836.8125 2978718.8125 3107144.8125 2062815.8125 -431.1875 145.8125 4262126 2359954 4646159 2589300 6456454 872274.8125 3122496.8125 2887606.8125 2561597.8125 1799781.8125 -43.1875 -149.1875 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 109.500 159.290 A6 25248449.813 18159491.212 5378553.346 29.6 137.542 A7 22187555.813 71.754 A8 12928255.813 77.296 A9 13708347.813 98.729 A10 16724846.813 02 64.200 101.772 B6 17153167.813 11394120.213 4904025.136 43.0 92.314 B7 15822017.813 50.203 B8 9895055.813 20.674 B9 5739051.813 39.305 B10 8361307.813 03 38.200 80.816 C6 14203650.813 8436064.813 4214976.771 50.0 62.365 C7 11606804.813 23.134 C8 6085338.813 12.806 C9 4631712.813 20.061 C10 5652816.813 04 21.600 105.095 D6 17620849.813 7319594.612 5824379.435 79.6 23.296 D7 6108178.813 12.823 D8 4634099.813 12.291 D9 4559292.813 6.012 D10 3675551.813 05 13.900 48.839 E6 9703147.813 5420043.013 3738019.928 69.0 45.954 E7 9297091.813 -0.504 E8 2758344.813 -3.940 E9 2274876.813 1.687 E10 3066753.813 06 7.000 5.395 F6 3588620.813 2697710.212 660984.219 24.5 1.492 F7 3039388.813 -2.197 F8 2520167.813 -7.191 F9 1817304.813 -2.176 F10 2523068.813 07 4.600 20.379 G6 5697598.813 3932423.013 1713852.388 43.6 21.219 G7 5815836.813 1.061 G8 2978718.813 1.974 G9 3107144.813 -5.446 G10 2062815.813 08 3.700 -13.905 H6 872274.813 2248751.612 917292.435 40.8 2.083 H7 3122496.813 0.414 H8 2887606.813 -1.902 H9 2561597.813 -7.315 H10 1799781.813 Group Summaries MinStd Smallest standard value: 2248751.612 Min(MeanValue) MaxStd Largest standard value: 18159491.212 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 1650361.000 R -8.377 -8.377 0.000 0.0 02 B1 892229.000 R -13.763 -13.763 0.000 0.0 03 C1 3242448.000 2.935 2.935 0.000 0.0 04 D1 2850205.000 0.148 0.148 0.000 0.0 05 E1 4130714.000 9.246 9.246 0.000 0.0 06 F1 41697.000 R -19.807 -19.807 0.000 0.0 07 G1 2825453.000 -0.028 -0.028 0.000 0.0 08 H1 4262126.000 10.180 10.180 0.000 0.0 09 A2 2558846.000 -1.922 -1.922 0.000 0.0 10 B2 1551306.000 R -9.081 -9.081 0.000 0.0 11 C2 1615025.000 R -8.628 -8.628 0.000 0.0 12 D2 4779826.000 13.858 13.858 0.000 0.0 13 E2 3734425.000 6.431 6.431 0.000 0.0 14 F2 1292236.000 R -10.921 -10.921 0.000 0.0 15 G2 2552460.000 -1.967 -1.967 0.000 0.0 16 H2 2359954.000 -3.335 -3.335 0.000 0.0 17 A3 7285444.000 31.661 31.661 0.000 0.0 18 B3 779149.000 R -14.567 -14.567 0.000 0.0 19 C3 21964.000 R -19.947 -19.947 0.000 0.0 20 D3 25304.000 R -19.923 -19.923 0.000 0.0 21 E3 4565833.000 12.338 12.338 0.000 0.0 22 F3 7274447.000 31.583 31.583 0.000 0.0 23 G3 3016317.000 1.328 1.328 0.000 0.0 24 H3 4646159.000 12.909 12.909 0.000 0.0 25 A4 2008621.000 R -5.831 -5.831 0.000 0.0 26 B4 611264.000 R -15.760 -15.760 0.000 0.0 27 C4 1803356.000 R -7.290 -7.290 0.000 0.0 28 D4 724133.000 R -14.958 -14.958 0.000 0.0 29 E4 635034.000 R -15.591 -15.591 0.000 0.0 30 F4 2006880.000 R -5.844 -5.844 0.000 0.0 31 G4 2415328.000 -2.942 -2.942 0.000 0.0 32 H4 2589300.000 -1.706 -1.706 0.000 0.0 33 A5 1747421.000 R -7.687 -7.687 0.000 0.0 34 B5 2326942.000 -3.570 -3.570 0.000 0.0 35 C5 2902847.000 0.522 0.522 0.000 0.0 36 D5 2531503.000 -2.116 -2.116 0.000 0.0 37 E5 995884.000 R -13.027 -13.027 0.000 0.0 38 F5 2772278.000 -0.405 -0.405 0.000 0.0 39 G5 379347.000 R -17.407 -17.407 0.000 0.0 40 H5 6456454.000 25.771 25.771 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 25 1184342 832610 530343 969943 18331540.2083333 15230514.2083333 11807197.2083333 20601264.2083333 15943045.2083333 950.208333333333 -315.791666666667 -224.791666666667 1075321 1238734 345197 1497964 9020500.20833333 5899370.20833333 4432325.20833333 11134379.2083333 6504046.20833333 677.208333333333 50.208333333333 -248.791666666667 1024808 651911 312975 14087 8272315.20833333 8146020.20833333 3174891.20833333 18298670.2083333 4751160.20833333 -427.791666666667 -100.791666666667 -0.79166666666697 1405186 1799529 880380 27073 10127296.2083333 5819576.20833333 4518371.20833333 8687888.20833333 5326931.20833333 272.208333333333 -67.791666666667 -218.791666666667 2075370 3575506 1540306 2238367 4510640.20833333 1792823.20833333 2568545.20833333 6653978.20833333 6628911.20833333 -609.791666666667 -631.791666666667 -244.791666666667 566642 4940688 662388 3749384 1455686.20833333 2186468.20833333 1041388.20833333 4721112.20833333 6374204.20833333 639.208333333333 -531.791666666667 -110.791666666667 383628 4225102 673560 1634551 2737856.20833333 1704055.20833333 1366386.20833333 3151481.20833333 3858077.20833333 -468.791666666667 6.20833333333303 -83.791666666667 757701 978981 1395485 3759711 2854963.20833333 1834689.20833333 988410.208333333 2655640.20833333 3088781.20833333 1006.20833333333 341.208333333333 344.208333333333 1 2 3 4 5 6 7 8 9 10 11 12 1184342 832610 530343 969943 18331540.208333332 15230514.208333334 11807197.208333334 20601264.208333332 15943045.208333334 950.208333333333 -315.79166666666697 -224.79166666666697 1075321 1238734 345197 1497964 9020500.208333334 5899370.208333333 4432325.208333333 11134379.208333334 6504046.208333333 677.208333333333 50.20833333333303 -248.79166666666697 1024808 651911 312975 14087 8272315.208333333 8146020.208333333 3174891.2083333335 18298670.208333332 4751160.208333333 -427.79166666666697 -100.79166666666697 -0.79166666666696983 1405186 1799529 880380 27073 10127296.208333334 5819576.208333333 4518371.208333333 8687888.208333334 5326931.208333333 272.20833333333303 -67.79166666666697 -218.79166666666697 2075370 3575506 1540306 2238367 4510640.208333333 1792823.2083333333 2568545.2083333335 6653978.208333333 6628911.208333333 -609.791666666667 -631.791666666667 -244.79166666666697 566642 4940688 662388 3749384 1455686.2083333333 2186468.2083333335 1041388.2083333334 4721112.208333333 6374204.208333333 639.208333333333 -531.791666666667 -110.79166666666697 383628 4225102 673560 1634551 2737856.2083333335 1704055.2083333333 1366386.2083333333 3151481.2083333335 3858077.2083333335 -468.79166666666697 6.20833333333303 -83.79166666666697 757701 978981 1395485 3759711 2854963.2083333335 1834689.2083333333 988410.20833333337 2655640.2083333335 3088781.2083333335 1006.208333333333 341.20833333333303 344.20833333333303 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 109.500 132.736 A5 18331540.208 16382712.208 3318520.618 20.3 106.652 A6 15230514.208 77.857 A7 11807197.208 151.828 A8 20601264.208 112.646 A9 15943045.208 02 64.200 54.417 B5 9020500.208 7398124.208 2666144.597 36.0 28.164 B6 5899370.208 15.824 B7 4432325.208 72.198 B8 11134379.208 33.250 B9 6504046.208 03 38.200 48.123 C5 8272315.208 8528611.408 5886373.465 69.0 47.061 C6 8146020.208 5.247 C7 3174891.208 132.460 C8 18298670.208 18.505 C9 4751160.208 04 21.600 63.727 D5 10127296.208 6896012.608 2394051.078 34.7 27.492 D6 5819576.208 16.547 D7 4518371.208 51.619 D8 8687888.208 23.348 D9 5326931.208 05 13.900 16.482 E5 4510640.208 4430979.608 2247637.815 50.7 -6.379 E6 1792823.208 0.146 E7 2568545.208 34.511 E8 6653978.208 34.300 E9 6628911.208 06 7.000 -9.214 F5 1455686.208 3155771.808 2297229.647 72.8 -3.067 F6 2186468.208 -12.699 F7 1041388.208 18.253 F8 4721112.208 32.158 F9 6374204.208 07 4.600 1.571 G5 2737856.208 2563571.208 1027591.710 40.1 -7.125 G6 1704055.208 -9.965 G7 1366386.208 5.050 G8 3151481.208 10.993 G9 3858077.208 08 3.700 2.556 H5 2854963.208 2284496.808 864901.741 37.9 -6.026 H6 1834689.208 -13.145 H7 988410.208 0.879 H8 2655640.208 4.522 H9 3088781.208 Group Summaries MinStd Smallest standard value: 2284496.808 Min(MeanValue) MaxStd Largest standard value: 16382712.208 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 1184342.000 R -11.497 -11.497 0.000 0.0 02 B1 1075321.000 R -12.414 -12.414 0.000 0.0 03 C1 1024808.000 R -12.839 -12.839 0.000 0.0 04 D1 1405186.000 R -9.639 -9.639 0.000 0.0 05 E1 2075370.000 R -4.002 -4.002 0.000 0.0 06 F1 566642.000 R -16.693 -16.693 0.000 0.0 07 G1 383628.000 R -18.232 -18.232 0.000 0.0 08 H1 757701.000 R -15.085 -15.085 0.000 0.0 09 A2 832610.000 R -14.455 -14.455 0.000 0.0 10 B2 1238734.000 R -11.039 -11.039 0.000 0.0 11 C2 651911.000 R -15.975 -15.975 0.000 0.0 12 D2 1799529.000 R -6.322 -6.322 0.000 0.0 13 E2 3575506.000 8.616 8.616 0.000 0.0 14 F2 4940688.000 20.100 20.100 0.000 0.0 15 G2 4225102.000 14.080 14.080 0.000 0.0 16 H2 978981.000 R -13.224 -13.224 0.000 0.0 17 A3 530343.000 R -16.998 -16.998 0.000 0.0 18 B3 345197.000 R -18.555 -18.555 0.000 0.0 19 C3 312975.000 R -18.826 -18.826 0.000 0.0 20 D3 880380.000 R -14.054 -14.054 0.000 0.0 21 E3 1540306.000 R -8.503 -8.503 0.000 0.0 22 F3 662388.000 R -15.887 -15.887 0.000 0.0 23 G3 673560.000 R -15.793 -15.793 0.000 0.0 24 H3 1395485.000 R -9.721 -9.721 0.000 0.0 25 A4 969943.000 R -13.300 -13.300 0.000 0.0 26 B4 1497964.000 R -8.859 -8.859 0.000 0.0 27 C4 14087.000 R -21.340 -21.340 0.000 0.0 28 D4 27073.000 R -21.231 -21.231 0.000 0.0 29 E4 2238367.000 R -2.631 -2.631 0.000 0.0 30 F4 3749384.000 10.079 10.079 0.000 0.0 31 G4 1634551.000 R -7.710 -7.710 0.000 0.0 32 H4 3759711.000 10.166 10.166 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 25.5 887550 1190415 7026386 854038 128535 13317574 14565257 5259160 9493835.125 20652160.125 17463404.125 14.125 947366 912744 1078042 990419 8569079 13494021 16740110 4935962 11704206.125 20625650.125 12999915.125 91.125 1283613 1066354 5066667 1548565 10273402 4799893 13411537 5088932 11189509.125 12341850.125 14409312.125 -143.875 204569 264322 3879587 1561560 8700161 6031760 4513887 5648684 17693686.125 8212493.125 7839572.125 -37.875 744652 1931855 4316001 1573451 7553113 4893113 6318127 6039322 12164332.125 5743943.125 4016652.125 358.125 336287 833270 3345312 2237120 6235048 7508706 16214419 6007861 229610.125 7131439.125 3327160.125 -211.875 659449 203284 2171341 3022746 6644710 7964754 9781579 2852741 5575878.125 3221751.125 3586317.125 -24.875 953938 128506 5489036 1922279 5182760 8362290 7913504 4403038 2746521.125 3585270.125 2753128.125 -44.875 1 2 3 4 5 6 7 8 9 10 11 12 887550 1190415 7026386 854038 128535 13317574 14565257 5259160 9493835.125 20652160.125 17463404.125 14.125 947366 912744 1078042 990419 8569079 13494021 16740110 4935962 11704206.125 20625650.125 12999915.125 91.125 1283613 1066354 5066667 1548565 10273402 4799893 13411537 5088932 11189509.125 12341850.125 14409312.125 -143.875 204569 264322 3879587 1561560 8700161 6031760 4513887 5648684 17693686.125 8212493.125 7839572.125 -37.875 744652 1931855 4316001 1573451 7553113 4893113 6318127 6039322 12164332.125 5743943.125 4016652.125 358.125 336287 833270 3345312 2237120 6235048 7508706 16214419 6007861 229610.125 7131439.125 3327160.125 -211.875 659449 203284 2171341 3022746 6644710 7964754 9781579 2852741 5575878.125 3221751.125 3586317.125 -24.875 953938 128506 5489036 1922279 5182760 8362290 7913504 4403038 2746521.125 3585270.125 2753128.125 -44.875 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 109.500 35.922 A9 9493835.125 15869799.792 5747324.184 36.2 126.262 A10 20652160.125 100.445 A11 17463404.125 02 64.200 53.818 B9 11704206.125 15109923.792 4820492.007 31.9 126.047 B10 20625650.125 64.308 B11 12999915.125 03 38.200 49.651 C9 11189509.125 12646890.458 1631431.900 12.9 58.980 C10 12341850.125 75.719 C11 14409312.125 04 21.600 102.309 D9 17693686.125 11248583.792 5584735.946 49.6 25.548 D10 8212493.125 22.529 D11 7839572.125 05 13.900 57.543 E9 12164332.125 7308309.125 4293204.291 58.7 5.563 E10 5743943.125 -8.422 E11 4016652.125 06 7.000 -39.082 F9 229610.125 3562736.458 3456939.838 97.0 16.796 F10 7131439.125 -14.004 F11 3327160.125 07 4.600 4.202 G9 5575878.125 4127982.125 1267094.792 30.7 -14.857 G10 3221751.125 -11.906 G11 3586317.125 08 3.700 -18.705 H9 2746521.125 3028306.458 482355.997 15.9 -11.914 H10 3585270.125 -18.651 H11 2753128.125 Group Summaries MinStd Smallest standard value: 3028306.458 Min(MeanValue) MaxStd Largest standard value: 15869799.792 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 887550.000 R -33.755 -33.755 0.000 0.0 02 B1 947366.000 R -33.271 -33.271 0.000 0.0 03 C1 1283613.000 R -30.549 -30.549 0.000 0.0 04 D1 204569.000 R -39.285 -39.285 0.000 0.0 05 E1 744652.000 R -34.912 -34.912 0.000 0.0 06 F1 336287.000 R -38.218 -38.218 0.000 0.0 07 G1 659449.000 R -35.602 -35.602 0.000 0.0 08 H1 953938.000 R -33.218 -33.218 0.000 0.0 09 A2 1190415.000 R -31.303 -31.303 0.000 0.0 10 B2 912744.000 R -33.551 -33.551 0.000 0.0 11 C2 1066354.000 R -32.308 -32.308 0.000 0.0 12 D2 264322.000 R -38.801 -38.801 0.000 0.0 13 E2 1931855.000 R -25.301 -25.301 0.000 0.0 14 F2 833270.000 R -34.195 -34.195 0.000 0.0 15 G2 203284.000 R -39.295 -39.295 0.000 0.0 16 H2 128506.000 R -39.901 -39.901 0.000 0.0 17 A3 7026386.000 15.945 15.945 0.000 0.0 18 B3 1078042.000 R -32.213 -32.213 0.000 0.0 19 C3 5066667.000 0.079 0.079 0.000 0.0 20 D3 3879587.000 -9.531 -9.531 0.000 0.0 21 E3 4316001.000 -5.998 -5.998 0.000 0.0 22 F3 3345312.000 -13.857 -13.857 0.000 0.0 23 G3 2171341.000 R -23.362 -23.362 0.000 0.0 24 H3 5489036.000 3.499 3.499 0.000 0.0 25 A4 854038.000 R -34.027 -34.027 0.000 0.0 26 B4 990419.000 R -32.923 -32.923 0.000 0.0 27 C4 1548565.000 R -28.404 -28.404 0.000 0.0 28 D4 1561560.000 R -28.299 -28.299 0.000 0.0 29 E4 1573451.000 R -28.202 -28.202 0.000 0.0 30 F4 2237120.000 R -22.829 -22.829 0.000 0.0 31 G4 3022746.000 R -16.469 -16.469 0.000 0.0 32 H4 1922279.000 R -25.378 -25.378 0.000 0.0 33 A5 128535.000 R -39.900 -39.900 0.000 0.0 34 B5 8569079.000 28.435 28.435 0.000 0.0 35 C5 10273402.000 42.234 42.234 0.000 0.0 36 D5 8700161.000 29.497 29.497 0.000 0.0 37 E5 7553113.000 20.210 20.210 0.000 0.0 38 F5 6235048.000 9.539 9.539 0.000 0.0 39 G5 6644710.000 12.855 12.855 0.000 0.0 40 H5 5182760.000 1.019 1.019 0.000 0.0 41 A6 13317574.000 66.880 66.880 0.000 0.0 42 B6 13494021.000 68.308 68.308 0.000 0.0 43 C6 4799893.000 -2.081 -2.081 0.000 0.0 44 D6 6031760.000 7.893 7.893 0.000 0.0 45 E6 4893113.000 -1.326 -1.326 0.000 0.0 46 F6 7508706.000 19.850 19.850 0.000 0.0 47 G6 7964754.000 23.543 23.543 0.000 0.0 48 H6 8362290.000 26.761 26.761 0.000 0.0 49 A7 14565257.000 76.981 76.981 0.000 0.0 50 B7 16740110.000 R 94.589 94.589 0.000 0.0 51 C7 13411537.000 67.641 67.641 0.000 0.0 52 D7 4513887.000 -4.396 -4.396 0.000 0.0 53 E7 6318127.000 10.211 10.211 0.000 0.0 54 F7 16214419.000 R 90.333 90.333 0.000 0.0 55 G7 9781579.000 38.252 38.252 0.000 0.0 56 H7 7913504.000 23.128 23.128 0.000 0.0 57 A8 5259160.000 1.638 1.638 0.000 0.0 58 B8 4935962.000 -0.979 -0.979 0.000 0.0 59 C8 5088932.000 0.260 0.260 0.000 0.0 60 D8 5648684.000 4.791 4.791 0.000 0.0 61 E8 6039322.000 7.954 7.954 0.000 0.0 62 F8 6007861.000 7.699 7.699 0.000 0.0 63 G8 2852741.000 R -17.845 -17.845 0.000 0.0 64 H8 4403038.000 -5.294 -5.294 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: 11-21-14_quantification; Date Last Saved: 11/21/2014 12:28:11 PM