##BLOCKS= 6 Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 -50073 -40366 -45405 -58018 -50315 -50945 -55103 -54856 -55009 -53114 -53712 -56698 -56193 -54425 -55551 -57834 -57836 -54837 -56986 -56494 -55999 -57490 -51253 -53001 179625 24133 -8333 -10927 -44165 -50487 -47897 -44839 -40853 -46414 -41567 -48271 17793701 14005747 8803810 5491900 3390064 1985121 1178040 707059 440670 296993 181733 0 -59307 -59223 -58231 -59314 -59279 -59303 -59442 -59264 -59353 -59137 -59334 -59327 -59317 -59374 -59333 -59311 -59321 -59244 -54695 -59284 -58664 -59164 -59315 -59441 -58807 -57784 -59329 -58947 -58974 -59233 -59089 -59260 -59253 -59277 -59392 -58843 -59449 -59384 -59372 -59269 -59295 -59317 -59319 -59345 -59319 -59364 -59345 -59373 ~End Plate: Plate2 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 14610399 13737354 13839426 11357231 13700789 16019527 13363147 12637294 11355865 12432221 14829021 14036069 14815973 11090782 12305796 14507057 14223508 14722041 10828522 12264790 15094927 12909372 3877356 14872586 10886793 14710195 13097148 14384361 13143341 13719165 13777740 13560050 10050242 9598096 12265707 13933944 12475969 15221008 9726611 14263628 13928606 15275065 14133136 11319742 13401385 15001141 13040952 11975740 12122993 13345117 14195558 13556434 13832968 11666573 9120283 13656298 13917239 13850355 12780544 13585899 17138882 14429157 14673244 10222751 12515080 15182583 12914857 9046820 12145808 13303401 12540733 9469608 17337400 12250865 16012177 16536376 17176666 16388463 11996988 12755070 13103872 12907497 10893333 11257225 15745738 16398344 14578351 14364859 12663830 14168509 15354071 15550902 14941363 10411440 15109716 14631947 ~End Plate: Plate3 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 11857386 11552012 14192645 16346081 13016777 17112140 5493064 3598785 14942168 14471399 13015660 16472439 10881507 13474699 10951846 13625127 10959661 14111860 13131041 8130697 13643740 14253930 13986077 13882627 12245136 13111468 10183417 13367094 14700485 9839400 12579725 13447801 11171978 12929962 13288543 11814942 10208668 11406700 14543759 11712262 13683895 10887060 13222976 12137766 11123324 14265206 15180607 13076724 11791346 11396140 13970077 11468651 11878314 13535687 12271490 12319799 11841048 15587416 10311011 8247197 11175771 11605563 14197630 14863083 10176250 13585069 12879001 8066778 15648080 14980798 11011852 14418811 11321241 14694172 14132445 14621088 10873125 14801551 12753133 5400217 15343269 13895365 8718323 16441737 11263902 15157455 12128614 14823029 17669900 14857965 7732409 10579324 15748238 14353802 11632181 18780700 ~End Plate: Plate4 1.3 PlateFormat Endpoint Fluorescence FALSE Reduced FALSE 1 1 535 1 12 96 485 Manual 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 13753986 26700628 14317689 19576322 1158 948 998 1410 1042 1127 1047 983 14643095 13365865 14560421 17791608 1027 1046 1091 1084 2084 1018 1052 1087 14427217 12258915 14906710 18049238 1102 1026 1071 1229 1075 1042 981 958 13826957 10320112 15583432 1039 1044 1086 1074 1089 1126 889 969 1014 16132115 12171171 17251806 1007 1026 1099 1167 1120 944 1054 1091 1037 15408324 13502911 17915380 1236 4984 1016 1128 1086 1028 1037 1063 1021 14737607 16791628 18881382 1083 1073 4958 1073 1050 1010 1082 993 1009 13923543 18079348 20433192 1034 3725 3230 1100 1095 1103 1105 1028 1136 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 300.000 282.472 D1 17793701.000 17793701.000 0.000 0.0 02 200.000 223.089 D2 14005747.000 14005747.000 0.000 0.0 03 133.333 141.538 D3 8803810.000 8803810.000 0.000 0.0 04 88.889 89.617 D4 5491900.000 5491900.000 0.000 0.0 05 59.259 56.667 D5 3390064.000 3390064.000 0.000 0.0 06 39.506 34.642 D6 1985121.000 1985121.000 0.000 0.0 07 26.337 21.989 D7 1178040.000 1178040.000 0.000 0.0 08 17.558 14.605 D8 707059.000 707059.000 0.000 0.0 09 11.706 10.429 D9 440670.000 440670.000 0.000 0.0 10 7.804 8.177 D10 296993.000 296993.000 0.000 0.0 11 5.202 6.370 D11 181733.000 181733.000 0.000 0.0 Group Summaries MinStd Smallest standard value: 181733.000 Min(MeanValue) MaxStd Largest standard value: 17793701.000 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 14610399.000 232.568 232.568 0.000 0.0 02 B1 14815973.000 235.791 235.791 0.000 0.0 03 C1 10886793.000 174.193 174.193 0.000 0.0 04 D1 12475969.000 199.106 199.106 0.000 0.0 05 E1 12122993.000 193.573 193.573 0.000 0.0 06 F1 17138882.000 272.207 272.207 0.000 0.0 07 G1 17337400.000 275.319 275.319 0.000 0.0 08 H1 15745738.000 250.366 250.366 0.000 0.0 09 A2 13737354.000 218.881 218.881 0.000 0.0 097 A1 11857386.000 189.409 189.409 0.000 0.0 098 B1 10881507.000 174.110 174.110 0.000 0.0 099 C1 12245136.000 195.488 195.488 0.000 0.0 10 B2 11090782.000 177.391 177.391 0.000 0.0 100 D1 10208668.000 163.562 163.562 0.000 0.0 101 E1 11791346.000 188.374 188.374 0.000 0.0 102 F1 11175771.000 178.723 178.723 0.000 0.0 103 G1 11321241.000 181.004 181.004 0.000 0.0 104 H1 11263902.000 180.105 180.105 0.000 0.0 105 A2 11552012.000 184.622 184.622 0.000 0.0 106 B2 13474699.000 214.763 214.763 0.000 0.0 107 C2 13111468.000 209.069 209.069 0.000 0.0 108 D2 11406700.000 182.343 182.343 0.000 0.0 109 E2 11396140.000 182.178 182.178 0.000 0.0 11 C2 14710195.000 234.132 234.132 0.000 0.0 110 F2 11605563.000 185.461 185.461 0.000 0.0 111 G2 14694172.000 233.881 233.881 0.000 0.0 112 H2 15157455.000 241.144 241.144 0.000 0.0 113 A3 14192645.000 226.019 226.019 0.000 0.0 114 B3 10951846.000 175.213 175.213 0.000 0.0 115 C3 10183417.000 163.166 163.166 0.000 0.0 116 D3 14543759.000 231.523 231.523 0.000 0.0 117 E3 13970077.000 222.529 222.529 0.000 0.0 118 F3 14197630.000 226.097 226.097 0.000 0.0 119 G3 14132445.000 225.075 225.075 0.000 0.0 12 D2 15221008.000 242.140 242.140 0.000 0.0 120 H3 12128614.000 193.661 193.661 0.000 0.0 121 A4 16346081.000 259.778 259.778 0.000 0.0 122 B4 13625127.000 217.122 217.122 0.000 0.0 123 C4 13367094.000 213.077 213.077 0.000 0.0 124 D4 11712262.000 187.134 187.134 0.000 0.0 125 E4 11468651.000 183.315 183.315 0.000 0.0 126 F4 14863083.000 236.529 236.529 0.000 0.0 127 G4 14621088.000 232.735 232.735 0.000 0.0 128 H4 14823029.000 235.901 235.901 0.000 0.0 129 A5 13016777.000 207.585 207.585 0.000 0.0 13 E2 13345117.000 212.732 212.732 0.000 0.0 130 B5 10959661.000 175.335 175.335 0.000 0.0 131 C5 14700485.000 233.980 233.980 0.000 0.0 132 D5 13683895.000 218.043 218.043 0.000 0.0 133 E5 11878314.000 189.737 189.737 0.000 0.0 134 F5 10176250.000 163.054 163.054 0.000 0.0 135 G5 10873125.000 173.979 173.979 0.000 0.0 136 H5 17669900.000 280.532 280.532 0.000 0.0 137 A6 17112140.000 271.788 271.788 0.000 0.0 138 B6 14111860.000 224.752 224.752 0.000 0.0 139 C6 9839400.000 157.773 157.773 0.000 0.0 14 F2 14429157.000 229.726 229.726 0.000 0.0 140 D6 10887060.000 174.197 174.197 0.000 0.0 141 E6 13535687.000 215.720 215.720 0.000 0.0 142 F6 13585069.000 216.494 216.494 0.000 0.0 143 G6 14801551.000 235.564 235.564 0.000 0.0 144 H6 14857965.000 236.449 236.449 0.000 0.0 145 A7 5493064.000 89.636 89.636 0.000 0.0 146 B7 13131041.000 209.376 209.376 0.000 0.0 147 C7 12579725.000 200.733 200.733 0.000 0.0 148 D7 13222976.000 210.817 210.817 0.000 0.0 149 E7 12271490.000 195.901 195.901 0.000 0.0 15 G2 12250865.000 195.577 195.577 0.000 0.0 150 F7 12879001.000 205.425 205.425 0.000 0.0 151 G7 12753133.000 203.451 203.451 0.000 0.0 152 H7 7732409.000 124.742 124.742 0.000 0.0 153 A8 3598785.000 59.939 59.939 0.000 0.0 154 B8 8130697.000 130.986 130.986 0.000 0.0 155 C8 13447801.000 214.342 214.342 0.000 0.0 156 D8 12137766.000 193.804 193.804 0.000 0.0 157 E8 12319799.000 196.658 196.658 0.000 0.0 158 F8 8066778.000 129.984 129.984 0.000 0.0 159 G8 5400217.000 88.180 88.180 0.000 0.0 16 H2 16398344.000 260.597 260.597 0.000 0.0 160 H8 10579324.000 169.373 169.373 0.000 0.0 161 A9 14942168.000 237.769 237.769 0.000 0.0 162 B9 13643740.000 217.413 217.413 0.000 0.0 163 C9 11171978.000 178.664 178.664 0.000 0.0 164 D9 11123324.000 177.901 177.901 0.000 0.0 165 E9 11841048.000 189.153 189.153 0.000 0.0 166 F9 15648080.000 248.835 248.835 0.000 0.0 167 G9 15343269.000 244.057 244.057 0.000 0.0 168 H9 15748238.000 250.406 250.406 0.000 0.0 169 A10 14471399.000 230.389 230.389 0.000 0.0 17 A3 13839426.000 220.481 220.481 0.000 0.0 170 B10 14253930.000 226.979 226.979 0.000 0.0 171 C10 12929962.000 206.224 206.224 0.000 0.0 172 D10 14265206.000 227.156 227.156 0.000 0.0 173 E10 15587416.000 247.884 247.884 0.000 0.0 174 F10 14980798.000 238.375 238.375 0.000 0.0 175 G10 13895365.000 221.358 221.358 0.000 0.0 176 H10 14353802.000 228.545 228.545 0.000 0.0 177 A11 13015660.000 207.567 207.567 0.000 0.0 178 B11 13986077.000 222.780 222.780 0.000 0.0 179 C11 13288543.000 211.845 211.845 0.000 0.0 18 B3 12305796.000 196.439 196.439 0.000 0.0 180 D11 15180607.000 241.507 241.507 0.000 0.0 181 E11 10311011.000 165.166 165.166 0.000 0.0 182 F11 11011852.000 176.153 176.153 0.000 0.0 183 G11 8718323.000 140.198 140.198 0.000 0.0 184 H11 11632181.000 185.878 185.878 0.000 0.0 185 A12 16472439.000 261.759 261.759 0.000 0.0 186 B12 13882627.000 221.159 221.159 0.000 0.0 187 C12 11814942.000 188.743 188.743 0.000 0.0 188 D12 13076724.000 208.524 208.524 0.000 0.0 189 E12 8247197.000 132.812 132.812 0.000 0.0 19 C3 13097148.000 208.845 208.845 0.000 0.0 190 F12 14418811.000 229.564 229.564 0.000 0.0 191 G12 16441737.000 261.278 261.278 0.000 0.0 192 H12 18780700.000 R 297.945 297.945 0.000 0.0 193 A1 13753986.000 219.142 219.142 0.000 0.0 194 B1 14643095.000 233.080 233.080 0.000 0.0 195 C1 14427217.000 229.696 229.696 0.000 0.0 196 D1 13826957.000 220.286 220.286 0.000 0.0 197 E1 16132115.000 256.424 256.424 0.000 0.0 198 F1 15408324.000 245.077 245.077 0.000 0.0 199 G1 14737607.000 234.562 234.562 0.000 0.0 20 D3 9726611.000 156.005 156.005 0.000 0.0 200 H1 13923543.000 221.800 221.800 0.000 0.0 201 A2 26700628.000 R 422.106 422.106 0.000 0.0 202 B2 13365865.000 213.057 213.057 0.000 0.0 203 C2 12258915.000 195.704 195.704 0.000 0.0 204 D2 10320112.000 165.309 165.309 0.000 0.0 205 E2 12171171.000 194.328 194.328 0.000 0.0 206 F2 13502911.000 215.206 215.206 0.000 0.0 207 G2 16791628.000 266.763 266.763 0.000 0.0 208 H2 18079348.000 R 286.950 286.950 0.000 0.0 209 A3 14317689.000 227.979 227.979 0.000 0.0 21 E3 14195558.000 226.064 226.064 0.000 0.0 210 B3 14560421.000 231.784 231.784 0.000 0.0 211 C3 14906710.000 237.213 237.213 0.000 0.0 212 D3 15583432.000 247.822 247.822 0.000 0.0 213 E3 17251806.000 273.977 273.977 0.000 0.0 214 F3 17915380.000 R 284.380 284.380 0.000 0.0 215 G3 18881382.000 R 299.524 299.524 0.000 0.0 216 H3 20433192.000 R 323.852 323.852 0.000 0.0 217 A4 19576322.000 R 310.418 310.418 0.000 0.0 218 B4 17791608.000 282.440 282.440 0.000 0.0 219 C4 18049238.000 R 286.478 286.478 0.000 0.0 22 F3 14673244.000 233.553 233.553 0.000 0.0 23 G3 16012177.000 254.543 254.543 0.000 0.0 24 H3 14578351.000 232.065 232.065 0.000 0.0 25 A4 11357231.000 181.568 181.568 0.000 0.0 26 B4 14507057.000 230.948 230.948 0.000 0.0 27 C4 14384361.000 229.024 229.024 0.000 0.0 28 D4 14263628.000 227.131 227.131 0.000 0.0 29 E4 13556434.000 216.045 216.045 0.000 0.0 30 F4 10222751.000 163.783 163.783 0.000 0.0 31 G4 16536376.000 262.761 262.761 0.000 0.0 32 H4 14364859.000 228.718 228.718 0.000 0.0 33 A5 13700789.000 218.308 218.308 0.000 0.0 34 B5 14223508.000 226.502 226.502 0.000 0.0 35 C5 13143341.000 209.569 209.569 0.000 0.0 36 D5 13928606.000 221.879 221.879 0.000 0.0 37 E5 13832968.000 220.380 220.380 0.000 0.0 38 F5 12515080.000 199.720 199.720 0.000 0.0 39 G5 17176666.000 272.799 272.799 0.000 0.0 40 H5 12663830.000 202.051 202.051 0.000 0.0 41 A6 16019527.000 254.659 254.659 0.000 0.0 42 B6 14722041.000 234.318 234.318 0.000 0.0 43 C6 13719165.000 218.596 218.596 0.000 0.0 44 D6 15275065.000 242.988 242.988 0.000 0.0 45 E6 11666573.000 186.417 186.417 0.000 0.0 46 F6 15182583.000 241.538 241.538 0.000 0.0 47 G6 16388463.000 260.442 260.442 0.000 0.0 48 H6 14168509.000 225.640 225.640 0.000 0.0 49 A7 13363147.000 213.015 213.015 0.000 0.0 50 B7 10828522.000 173.279 173.279 0.000 0.0 51 C7 13777740.000 219.514 219.514 0.000 0.0 52 D7 14133136.000 225.086 225.086 0.000 0.0 53 E7 9120283.000 146.499 146.499 0.000 0.0 54 F7 12914857.000 205.987 205.987 0.000 0.0 55 G7 11996988.000 191.597 191.597 0.000 0.0 56 H7 15354071.000 244.226 244.226 0.000 0.0 57 A8 12637294.000 201.635 201.635 0.000 0.0 58 B8 12264790.000 195.796 195.796 0.000 0.0 59 C8 13560050.000 216.101 216.101 0.000 0.0 60 D8 11319742.000 180.980 180.980 0.000 0.0 61 E8 13656298.000 217.610 217.610 0.000 0.0 62 F8 9046820.000 145.348 145.348 0.000 0.0 63 G8 12755070.000 203.482 203.482 0.000 0.0 64 H8 15550902.000 247.312 247.312 0.000 0.0 65 A9 11355865.000 181.547 181.547 0.000 0.0 66 B9 15094927.000 240.164 240.164 0.000 0.0 67 C9 10050242.000 161.078 161.078 0.000 0.0 68 D9 13401385.000 213.614 213.614 0.000 0.0 69 E9 13917239.000 221.701 221.701 0.000 0.0 70 F9 12145808.000 193.930 193.930 0.000 0.0 71 G9 13103872.000 208.950 208.950 0.000 0.0 72 H9 14941363.000 237.756 237.756 0.000 0.0 73 A10 12432221.000 198.421 198.421 0.000 0.0 74 B10 12909372.000 205.901 205.901 0.000 0.0 75 C10 9598096.000 153.990 153.990 0.000 0.0 76 D10 15001141.000 238.693 238.693 0.000 0.0 77 E10 13850355.000 220.653 220.653 0.000 0.0 78 F10 13303401.000 212.078 212.078 0.000 0.0 79 G10 12907497.000 205.871 205.871 0.000 0.0 80 H10 10411440.000 166.741 166.741 0.000 0.0 81 A11 14829021.000 235.995 235.995 0.000 0.0 82 B11 3877356.000 64.306 64.306 0.000 0.0 83 C11 12265707.000 195.810 195.810 0.000 0.0 84 D11 13040952.000 207.964 207.964 0.000 0.0 85 E11 12780544.000 203.881 203.881 0.000 0.0 86 F11 12540733.000 200.122 200.122 0.000 0.0 87 G11 10893333.000 174.295 174.295 0.000 0.0 88 H11 15109716.000 240.396 240.396 0.000 0.0 89 A12 14036069.000 223.564 223.564 0.000 0.0 90 B12 14872586.000 236.678 236.678 0.000 0.0 91 C12 13933944.000 221.963 221.963 0.000 0.0 92 D12 11975740.000 191.264 191.264 0.000 0.0 93 E12 13585899.000 216.507 216.507 0.000 0.0 94 F12 9469608.000 151.976 151.976 0.000 0.0 95 G12 11257225.000 180.000 180.000 0.000 0.0 96 H12 14631947.000 232.906 232.906 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: HiSeq2063_RNAConc_01-21-2020; Date Last Saved: 1/21/2020 4:01:10 PM