Load time: 4/4/2011 16:36 Read time: 4/4/2011 16:36 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1423 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10611086 cps Min counts: 55070 counts Well list: "r0(a1:h4,a7:h10) = cps(f2(a1:h4,a7:h10))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10611086 4283001 3840857 127417 4185070 3615286 3754518 112649 B 2432134 2560208 1806654 120479 1753047 1477517 1153291 111584 C 1750430 1470484 1647051 119396 1134969 947664 687337 110140 D 2006979 1165151 839177 117153 833703 697904 519209 113504 E 820532 556214 728504 115500 596754 681711 709175 119016 F 449608 359900 435924 112174 364727 532057 382346 117229 G 465117 278781 288056 114701 367635 238983 213932 114302 H 232577 390176 359120 118788 269963 225374 216859 140740 Load time: 4/4/2011 16:40 Read time: 4/4/2011 16:40 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1424 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 15389299 cps Min counts: 82068 counts Well list: "r0(a1:h4,a7:h10,a5:h6,a11:h12) = cps(f2(a1:h4,a7:h10,a5:h6,a11:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15389299 14016658 4592635 2207679 246509 2751127 217354 827792 205855 1153538 748385 1226199 B 11842469 8061714 1993579 1634573 302520 1687965 332835 1019640 1420783 1201415 984840 237272 C 6915535 2253925 1805647 1455089 1501426 1557753 430621 416747 939719 1232319 451395 1111269 D 6065770 6803116 2341787 608386 1530468 1304580 446872 1049651 715903 1207269 657554 888479 E 9093000 4483823 2031374 815552 1153728 1343600 765566 883689 955190 822813 822414 969616 F 11860006 2598857 1323662 1079605 1219566 1225762 659854 920523 976268 996510 520729 546388 G 11065652 525348 1214339 1564921 1498328 164136 900073 941886 1032849 748898 724285 1157264 H 10684221 337206 1868943 505296 2098297 2142355 852386 684467 685703 1085344 717101 471123 Load time: 4/4/2011 16:42 Read time: 4/4/2011 16:42 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1425 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 3185224 cps Min counts: 90241 counts Well list: "r0(a1:h4,a7:h10,a5:h6,a11:h12) = cps(f2(a1:h4,a7:h10,a5:h6,a11:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1617120 1997416 1378343 1070025 1328927 1923267 1087264 1240453 2631475 192684 2377864 180482 B 1456439 2245004 648603 1955950 1894051 2235265 1902136 1718511 1923110 1211868 1964894 1833096 C 1086827 456470 1620265 1930480 1772687 1521364 1103989 678651 2116596 1753676 559179 1035624 D 2343751 1975052 2033542 2125230 1997525 598179 3185224 2415431 1455299 2184769 1082189 916893 E 875840 2212712 2199137 2671614 1135768 976173 2790846 1214662 2236786 1350651 1995005 2027154 F 181584 1658144 1504277 1797418 1175833 2439252 2542539 2719591 2709568 1461304 294937 707712 G 550550 1939400 1149775 2035672 785295 2330316 1572418 2253677 2502903 1696702 1235341 2099318 H 2357707 1799308 1919058 1972258 1666645 1019355 2323193 1218425 2353952 1548809 1522086 1036936 Load time: 4/4/2011 16:45 Read time: 4/4/2011 16:45 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1426 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 7434035 cps Min counts: 63271 counts Well list: "r0(a1:h4,a7:h10,a5:h6,a11:h12) = cps(f2(a1:h4,a7:h10,a5:h6,a11:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1842660 1352076 1380509 672132 3644125 3572043 1981549 1590892 1481869 1429412 1911103 1612623 B 1918444 136064 515617 155698 4972202 7434035 2619392 2181124 1225819 3528438 1578018 2090304 C 1816437 192836 1557642 1515738 2222476 3481544 1132670 554180 1867332 1302603 441987 3067743 D 610229 592211 1801960 1358386 2204658 930748 2882445 2419237 615551 1972415 1065597 1251021 E 1270046 811542 1395714 1772289 1283331 752984 1135292 127683 834654 1570700 1604818 169895 F 1832874 704671 1360515 126542 1659417 3212750 1720776 2061526 1103248 194812 955970 2509499 G 1452030 3159798 1709739 1134950 1166501 5748382 819335 1627893 1228955 1339684 565546 2631366 H 4280319 1438725 981153 1680415 1112599 952701 1083843 927251 1830941 3633354 1859988 1701191 Load time: 4/4/2011 16:48 Read time: 4/4/2011 16:48 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1427 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 7751213 cps Min counts: 47097 counts Well list: "r0(a1:h4,a7:h10,a5:h6,a11:h12) = cps(f2(a1:h4,a7:h10,a5:h6,a11:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 107517 898875 675895 2043768 692317 1791797 1529289 3411279 4605103 5112293 6711978 7751213 B 94194 649401 758895 1631180 2645206 1088233 3386556 2117306 3981891 4762728 5817491 5171847 C 102157 594929 1909343 2542262 2691244 1474210 976116 1560006 3549944 4275219 6362906 6274766 D 108696 1990018 1127576 1574445 1593175 2545534 472948 1205425 1316782 3604976 6404764 4713772 E 120555 829712 1069569 1969433 3125091 1838745 510903 1261056 3318660 2824830 4318821 5813689 F 118997 412775 1755032 3345912 400915 508508 986170 687812 2815720 2498670 2724058 4559342 G 111014 2052081 1532368 2309499 1464726 1738776 2131988 1487533 2836524 1818163 5110417 5084350 H 105426 1802397 2283899 2624440 1178646 2351481 3426085 2102745 742607 2123123 4459037 5008671 Load time: 4/4/2011 16:51 Read time: 4/4/2011 16:51 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1428 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 13964536 cps Min counts: 20925 counts Well list: "r0(a1:h4,a7:h10,a5:h6,a11:h12) = cps(f2(a1:h4,a7:h10,a5:h6,a11:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13964536 5085529 5439430 130153 44664 42725 5008068 3841447 3776930 116184 45595 43371 B 4057463 2947033 2195482 124357 44607 43695 2029044 1740283 1309579 112858 43562 43942 C 2307503 1822350 1687827 124167 44170 45880 1356429 922994 807532 112630 43448 45139 D 3155612 1296331 1139892 123330 44037 44455 1516099 880059 630319 116659 42003 45367 E 1510511 868579 560794 119605 45443 46983 828343 737095 615532 119453 43428 42725 F 805726 876220 506893 116754 43448 44018 465935 464129 347184 118123 42820 42687 G 580390 372672 347127 128215 41851 43980 367236 239211 306055 114416 42592 42041 H 469280 677910 411425 134107 42706 44512 319454 342376 212051 147278 41927 42820 Load time: 4/4/2011 16:51 Read time: 4/4/2011 16:51 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1428 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 13964536 cps Min counts: 20925 counts Well list: "r0(a1:h4,a7:h10,a5:h6,a11:h12) = cps(f2(a1:h4,a7:h10,a5:h6,a11:h12))" Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13964536 5085529 5439430 130153 44664 42725 5008068 3841447 3776930 116184 45595 43371 B 4057463 2947033 2195482 124357 44607 43695 2029044 1740283 1309579 112858 43562 43942 C 2307503 1822350 1687827 124167 44170 45880 1356429 922994 807532 112630 43448 45139 D 3155612 1296331 1139892 123330 44037 44455 1516099 880059 630319 116659 42003 45367 E 1510511 868579 560794 119605 45443 46983 828343 737095 615532 119453 43428 42725 F 805726 876220 506893 116754 43448 44018 465935 464129 347184 118123 42820 42687 G 580390 372672 347127 128215 41851 43980 367236 239211 306055 114416 42592 42041 H 469280 677910 411425 134107 42706 44512 319454 342376 212051 147278 41927 42820 Load time: 4/4/2011 16:58 Read time: 4/4/2011 16:58 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1429 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 15975989 cps Min counts: 77754 counts Well list: "r0(a1:h4,a7:h10,a5:h6,a11:h12) = cps(f2(a1:h4,a7:h10,a5:h6,a11:h12))" Data: RAW DATA Units: cps Display format: %.2f