##BLOCKS= 4 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 12 96 485 Manual 0 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 25.5 7953278 1857680 207549 31693 8532699 1841433 192736 29339 8255772 1741668 175632 26836 326949 274380 349891 341200 374450 462678 376946 420486 670651 404039 527091 335028 437124 363369 453078 388108 384945 405245 345713 363136 275488 368498 298033 309893 810 840 841 851 836 858 779 841 919 896 844 912 835 785 895 815 914 860 857 745 824 847 822 934 860 897 972 898 885 865 821 816 848 896 829 804 860 805 889 835 852 800 829 884 908 821 970 784 847 845 845 924 818 902 811 821 947 917 868 821 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV standard 10.000 9.551 A1 7953278.000 8247249.667 289804.497 3.5 10.280 A5 8532699.000 9.932 A9 8255772.000 standard01 1.000 1.880 A2 1857680.000 1813593.667 62816.936 3.5 1.860 A6 1841433.000 1.734 A10 1741668.000 standard02 0.100 -0.196 A3 207549.000 191972.333 15972.198 8.3 -0.215 A7 192736.000 -0.236 A11 175632.000 standard03 0.010 -0.417 A4 31693.000 29289.333 2428.881 8.3 -0.420 A8 29339.000 -0.423 A12 26836.000 Group Summaries MinStd Smallest standard value: 29289.333 Min(MeanValue) MaxStd Largest standard value: 8247249.667 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 B1 326949.000 -0.046 -0.046 0.000 0.0 02 B2 274380.000 -0.112 -0.112 0.000 0.0 03 B3 349891.000 -0.017 -0.017 0.000 0.0 04 B4 341200.000 -0.028 -0.028 0.000 0.0 05 B5 374450.000 0.014 0.014 0.000 0.0 06 B6 462678.000 0.125 0.125 0.000 0.0 07 B7 376946.000 0.017 0.017 0.000 0.0 08 B8 420486.000 0.072 0.072 0.000 0.0 09 B9 670651.000 0.387 0.387 0.000 0.0 10 B10 404039.000 0.051 0.051 0.000 0.0 11 B11 527091.000 0.206 0.206 0.000 0.0 12 B12 335028.000 -0.036 -0.036 0.000 0.0 13 C1 437124.000 0.093 0.093 0.000 0.0 14 C2 363369.000 0.000 0.000 0.000 0.0 15 C3 453078.000 0.113 0.113 0.000 0.0 16 C4 388108.000 0.031 0.031 0.000 0.0 17 C5 384945.000 0.027 0.027 0.000 0.0 18 C6 405245.000 0.053 0.053 0.000 0.0 19 C7 345713.000 -0.022 -0.022 0.000 0.0 20 C8 363136.000 -0.000 -0.000 0.000 0.0 21 C9 275488.000 -0.111 -0.111 0.000 0.0 22 C10 368498.000 0.006 0.006 0.000 0.0 23 C11 298033.000 -0.082 -0.082 0.000 0.0 24 C12 309893.000 -0.067 -0.067 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: 122214; Date Last Saved: 12/22/2014 4:35:05 PM