Load time: 07/02/2013 12:49:28 PM Read time: 07/02/2013 12:49:28 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3077 Comment: 070213A Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 27.5 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 33741456 cps Min counts: 24770 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27829580.00 2812756.00 282937.00 234228.00 30725092.00 2781854.00 305600.00 49540.00 33741456.00 3038312.00 338337.00 68574.00 B 1462829.00 1148534.00 1396088.00 1625738.00 609462.00 1431396.00 1500821.00 1908817.00 1973200.00 1965796.00 1125777.00 1719290.00 C 1691629.00 1948814.00 1685734.00 1291581.00 1802317.00 2289363.00 1683127.00 1720773.00 2063780.00 1781668.00 1810308.00 1942207.00 D 1489386.00 1655152.00 352589.00 1490218.00 1303621.00 1859454.00 1294983.00 802314.00 1808166.00 2027407.00 2787533.00 807607.00 E 1635605.00 1121127.00 2166120.00 1684200.00 900867.00 1207753.00 1105930.00 1710140.00 1227562.00 1250810.00 1747946.00 1540817.00 F 1176149.00 751261.00 1529060.00 1781705.00 1401323.00 2077297.00 1283321.00 1329346.00 1238505.00 1628201.00 1328382.00 1243722.00 G 1336434.00 1160839.00 1147003.00 1592651.00 2470095.00 1692734.00 2153389.00 1624940.00 1336945.00 1186677.00 1021213.00 1366242.00 H 1459899.00 1418146.00 1049773.00 1188114.00 1289275.00 1269183.00 1767172.00 1501332.00 1145793.00 1075423.00 1353124.00 1014427.00