Load time: 07/02/2013 12:53:47 PM Read time: 07/02/2013 12:53:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3078 Comment: 070213B Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 27.7 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 24174658 cps Min counts: 23154 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 24014486.00 2491809.00 238065.00 47405.00 24174658.00 2400913.00 345104.00 46308.00 20547190.00 1922622.00 84036.00 234889.00 B 602940.00 975717.00 1242059.00 815205.00 1127137.00 1409886.00 993258.00 1146020.00 1297611.00 1616864.00 932565.00 1052306.00 C 853896.00 1374691.00 1070263.00 1142542.00 1129311.00 1565463.00 1446120.00 1427710.00 822936.00 1042610.00 1438522.00 1337455.00 D 931790.00 980178.00 1879225.00 1808645.00 1659160.00 786758.00 1211439.00 1479765.00 998739.00 1464530.00 1180723.00 1164487.00 E 1543123.00 1845105.00 1015940.00 1606681.00 995299.00 1442434.00 963166.00 1093852.00 693195.00 1185128.00 857166.00 981482.00 F 1076009.00 933963.00 1027413.00 1234385.00 1465305.00 1499460.00 1518986.00 1204199.00 1006489.00 1136361.00 1638925.00 755873.00 G 783356.00 1090676.00 1125115.00 1125531.00 952846.00 1377867.00 1309330.00 1406068.00 867732.00 1061644.00 1875165.00 1434798.00 H 1520498.00 1206638.00 1077483.00 1301259.00 2037985.00 2161354.00 2166666.00 1962955.00 1958930.00 1582096.00 1369266.00 1639463.00