Load time: 07/03/2013 09:47:53 AM Read time: 07/03/2013 09:47:54 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3081 Comment: 070313A Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 26.5 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 30522982 cps Min counts: 13155 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 989941.00 1551557.00 1230428.00 1768674.00 1575332.00 1704633.00 1624636.00 1883817.00 2293886.00 1673092.00 1628491.00 2092627.00 B 1542836.00 1399561.00 1641838.00 1949725.00 2174622.00 2186509.00 1731267.00 1515617.00 1983746.00 1602783.00 976369.00 970680.00 C 1406460.00 1525219.00 1800991.00 1615313.00 1709448.00 1662278.00 1814683.00 1599374.00 1690269.00 1167296.00 1319644.00 1873299.00 D 1494277.00 1303785.00 1163799.00 2265755.00 1573739.00 2203947.00 1314730.00 3034217.00 1409484.00 1889544.00 1670939.00 985083.00 E 1884379.00 1704682.00 1846494.00 1748916.00 2337612.00 2327090.00 1475867.00 1618453.00 1879833.00 1732656.00 1742836.00 1274469.00 F 1433943.00 1737371.00 1352023.00 999656.00 1555158.00 1520815.00 1894060.00 1886716.00 980395.00 1346428.00 1651456.00 1211129.00 G 1349622.00 1501932.00 2059648.00 1250464.00 1805722.00 1596884.00 1921246.00 1757249.00 1101423.00 1383078.00 1150247.00 1659943.00 H 24016586.00 2764354.00 341724.00 83772.00 30522982.00 2977846.00 85341.00 26311.00 26447748.00 2058305.00 236725.00 75323.00