Load time: 07/03/2013 09:51:01 AM Read time: 07/03/2013 09:51:01 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3082 Comment: 070313B Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 26.6 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 32525414 cps Min counts: 14025 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 29218986.00 3147437.00 376258.00 54739.00 31432564.00 797502.00 330572.00 28050.00 32525414.00 2545079.00 160060.00 39145.00 B 1520267.00 1191301.00 1951247.00 1834750.00 1238177.00 1783953.00 1249481.00 1806487.00 1472068.00 935617.00 708021.00 1742810.00 C 1053129.00 1228651.00 1332157.00 1130249.00 1376217.00 1242298.00 2101816.00 1460386.00 1573091.00 2230968.00 1122480.00 1087833.00 D 1547264.00 1926363.00 1354971.00 1810444.00 1098607.00 1391584.00 1001320.00 1718387.00 1537392.00 1127980.00 1273618.00 1043716.00 E 1109003.00 1367276.00 1957877.00 1094165.00 2035170.00 1408709.00 1300667.00 1021356.00 884847.00 873941.00 1111139.00 1387445.00 F 1233414.00 911933.00 897417.00 1532402.00 1373401.00 1198125.00 1689259.00 1239652.00 1253790.00 1411752.00 1333159.00 675718.00 G 1830087.00 1646818.00 1971630.00 1658871.00 1352457.00 1076908.00 1372890.00 1199183.00 1584463.00 2327478.00 985423.00 1090952.00 H 985518.00 1229502.00 1647873.00 1250558.00 1534632.00 1648965.00 1410939.00 1230825.00 1056664.00 1399617.00 1057269.00 1070878.00