##BLOCKS= 2 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Fluorescence FALSE Raw FALSE 1 1 535 1 24 384 485 Manual 0 1 16 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 22 5002538 4806712 43946 42821 1091 931 1079 988 968 1090 1301 1027 1044 996 1059 1150 999 1053 1058 1035 990 993 1152 1035 4859142 4859579 3579766 3558223 1354506 1326184 2328046 2257404 2535856 2188492 105011 97944 994 1108 1020 1046 884 1037 955 1063 944 1001 992 1080 2936460 2497553 26400 23585 978 1335 964 1185 958 1097 1082 1085 1082 1099 1110 1062 1082 1002 1033 1078 1022 985 1030 1004 4178848 4073771 331729 304026 870603 901688 3997259 4050440 4024847 4152826 671820 654223 1059 1062 1034 1060 1148 987 1072 1077 1117 1092 973 999 1390542 1357740 1839 1901 1105 1069 1040 964 1108 1040 1054 1087 1068 1076 1138 1157 968 1016 937 968 1047 976 1106 965 4217212 4187627 3394814 3248355 908823 935701 4157147 4226253 3873542 3821149 675588 639768 1008 960 1028 1149 983 1096 1079 1090 1029 1077 1068 1097 658644 681373 1809 1794 1192 1063 1189 1018 1309 1096 1035 1082 1023 1263 1093 1143 964 1073 1000 1056 1050 1002 932 1064 1714298 3490796 1497483 1501502 672635 654484 1820546 1901858 3338028 3241198 1669 1661 936 1087 1048 1004 979 1051 1001 1088 1057 1063 1016 1139 295556 276168 2022 1880 1082 1045 981 1078 1120 1037 945 1061 1122 1047 1119 1011 1044 1022 958 991 1138 1082 993 1773 554206 564062 2966102 2901719 379273 368412 1774258 1751830 3818987 3809084 1751 1709 964 987 1073 970 987 1103 993 984 1062 1068 1137 1085 215994 192604 2293 2381 1123 1164 982 1073 1089 1031 1100 1072 1020 992 983 1119 1083 1044 2435 1104 999 997 1046 1035 4032746 3979387 1300154 1269707 2055914 2034142 2468151 2388905 9447 9422 1863 1894 1103 1114 993 1084 1067 1007 990 982 1040 1101 1021 1140 124123 120708 1944 2090 1115 1041 1321 999 1032 1274 1741 1037 1042 1103 1012 1064 998 992 1003 1204 1042 1228 1242 1008 3556727 3638453 562213 673681 3143976 3253062 550036 508559 3730 3366 1939 1964 1018 999 1013 1477 1040 1038 928 1172 1046 1079 1082 978 72471 71670 2019 1972 1005 1041 1057 1021 1005 964 1027 1098 1059 977 1053 1062 1116 1436 1069 1034 913 1028 1061 1096 1883175 1808148 567214 552616 3761032 3712751 4700684 4246368 3101 3120 1619 1808 1070 1057 1046 975 1033 951 1003 1027 1052 995 1036 1112 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 ~End Original Filename: 101315_Tumor_Colombia; Date Last Saved: 10/13/2015 11:57:57 AM