Load time: 11/23/2010 02:14:04 PM Read time: 11/23/2010 02:14:04 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1194 Comment: standard, cowpea Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 35463952 cps Min counts: 894 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35463952.00 81312.00 441965.00 259536.00 509903.00 423853.00 587867.00 300002.00 216350.00 117611.00 281510.00 1959.00 B 3870951.00 340201.00 460667.00 351768.00 210433.00 111732.00 207789.00 178053.00 63466.00 62058.00 82700.00 1788.00 C 465271.00 224683.00 514107.00 278409.00 261572.00 292430.00 152483.00 45107.00 181744.00 56332.00 36223.00 1883.00 D 83766.00 228754.00 361300.00 221410.00 261762.00 185492.00 245058.00 461180.00 54981.00 232578.00 36013.00 1921.00 E 41664.00 97273.00 236649.00 207503.00 207351.00 308468.00 555315.00 688888.00 149116.00 147784.00 34415.00 1902.00 F 31505.00 381504.00 184027.00 691704.00 116317.00 763637.00 34929.00 280863.00 66130.00 82377.00 33084.00 2016.00 G 261648.00 101668.00 431064.00 205715.00 164488.00 183741.00 32950.00 69136.00 218804.00 45583.00 32304.00 1940.00 H 550882.00 449518.00 480509.00 215189.00 215380.00 166219.00 34739.00 61868.00 81331.00 51918.00 30687.00 1883.00 Load time: 11/23/2010 02:19:34 PM Read time: 11/23/2010 02:19:34 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Lyons_Lab Plate ID: #1195 Comment: new comment Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f4 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 7.05 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 4 A/D converter gain: x1 Integrating gain: x1 Max cps: 46646596 cps Min counts: 3451 counts Well list: r0(a1:h11) = (f4(a1:h11)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4664659600.00 10431000.00 58697900.00 32606400.00 69689200.00 60767700.00 78619000.00 37119800.00 26329600.00 15328600.00 34907700.00 B 512143700.00 44875800.00 59364100.00 43477900.00 28401300.00 14767800.00 23273700.00 24167100.00 8246300.00 8195800.00 10453400.00 C 57874100.00 30315000.00 71300000.00 34038200.00 30904700.00 37516400.00 20210800.00 5835700.00 23846100.00 7655300.00 4602300.00 D 10707100.00 31066300.00 49644000.00 24673500.00 28707000.00 23554000.00 33233600.00 60767700.00 7427100.00 33970500.00 4608300.00 E 5389800.00 12389000.00 32270200.00 25250100.00 25778500.00 38382800.00 72797700.00 76349700.00 19408600.00 22270500.00 4536600.00 F 4209900.00 48857700.00 22492400.00 85350900.00 14963200.00 102483400.00 4482800.00 38660100.00 8695400.00 11443000.00 4371400.00 G 35506900.00 12986000.00 58276700.00 25676500.00 22460500.00 25002700.00 4205600.00 8824200.00 28442500.00 5774300.00 4155100.00 H 73281400.00 61342200.00 66499300.00 29487100.00 24003100.00 20318000.00 4698800.00 7975100.00 10534900.00 6556100.00 4010900.00