Load time: 11/23/2010 02:26:53 PM Read time: 11/23/2010 02:26:53 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1196 Comment: standard, cowpea Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 34661200 cps Min counts: 894 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 34661200.00 76574.00 446227.00 244716.00 512091.00 349923.00 571867.00 259802.00 193520.00 107946.00 251089.00 1864.00 B 3832802.00 330118.00 443069.00 342541.00 218880.00 108365.00 177710.00 165344.00 57892.00 60213.00 79580.00 2016.00 C 470388.00 219964.00 529784.00 249339.00 225786.00 278694.00 139337.00 42843.00 171280.00 52413.00 34377.00 1826.00 D 80893.00 227137.00 363164.00 191770.00 208607.00 172326.00 238609.00 472519.00 52089.00 244659.00 33274.00 1826.00 E 40370.00 95295.00 226414.00 184255.00 191142.00 285429.00 546716.00 620323.00 140536.00 146985.00 31409.00 1921.00 F 31181.00 363393.00 165820.00 574987.00 113387.00 740693.00 32456.00 293857.00 62705.00 85782.00 30534.00 1845.00 G 262028.00 92726.00 419839.00 188897.00 157468.00 182885.00 31524.00 67462.00 202519.00 42900.00 29659.00 1826.00 H 549969.00 446874.00 477408.00 215056.00 206362.00 156193.00 32608.00 55723.00 72712.00 50320.00 29317.00 1788.00