Load time: 12/14/2011 04:11:11 PM Read time: 12/14/2011 04:11:11 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2063 Comment: p2_e2_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 76061824 cps Min counts: 19388 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 78106.00 73696.00 72194.00 40222.00 40678.00 41552.00 44905372.00 61434144.00 64670312.00 56762740.00 62285848.00 64739140.00 B 116826.00 116712.00 108653.00 42807.00 44118.00 44309.00 46890688.00 34551872.00 45555124.00 59770752.00 54361064.00 59240932.00 C 184269.00 188432.00 197024.00 40089.00 40412.00 40545.00 51231904.00 58015972.00 55983188.00 57389068.00 71953928.00 49989200.00 D 411117.00 303263.00 458639.00 43320.00 42978.00 41571.00 57404724.00 76061824.00 48458464.00 44329600.00 50856768.00 46073788.00 E 1190356.00 738122.00 1349229.00 44575.00 45069.00 42959.00 15110395.00 21103994.00 24057118.00 33093402.00 59014184.00 32472984.00 F 4063336.00 2503561.00 4380019.00 42104.00 45848.00 43339.00 58938236.00 45340772.00 45121892.00 35411780.00 65506592.00 32016964.00 G 9860047.00 8512278.00 12581268.00 45259.00 45373.00 43909.00 55436696.00 45989148.00 60071424.00 43829508.00 66152216.00 31988156.00 H 18571816.00 40526.00 17855684.00 38777.00 41248.00 41533.00 45032636.00 35005004.00 42224352.00 63336188.00 31911196.00 52866888.00 Load time: 12/14/2011 04:14:50 PM Read time: 12/14/2011 04:14:50 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2064 Comment: p2_e2_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 96194888 cps Min counts: 20434 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 57065612.00 72060464.00 51977844.00 37003204.00 43130.00 26920492.00 90290.00 77193.00 86432.00 43719.00 40868.00 42674.00 B 57073140.00 67660688.00 65180308.00 63614600.00 45810.00 37996212.00 136880.00 114735.00 125608.00 44841.00 46057.00 41400.00 C 81491240.00 31359804.00 64730532.00 61129308.00 35075176.00 46259940.00 247891.00 182482.00 225574.00 46038.00 44232.00 45962.00 D 40576804.00 50511328.00 48184956.00 89812288.00 93863920.00 35893368.00 537620.00 399275.00 513003.00 47597.00 45677.00 43776.00 E 38598604.00 60827004.00 57606468.00 66028796.00 14873324.00 43716792.00 190161.00 856793.00 1073282.00 46095.00 44404.00 45183.00 F 34861904.00 51713400.00 44270620.00 63926940.00 46456.00 38637296.00 4654712.00 3067179.00 4089811.00 44290.00 42845.00 46152.00 G 30281140.00 44746140.00 55351176.00 57148548.00 51283200.00 43231320.00 9654772.00 8949073.00 11959310.00 45297.00 46837.00 45031.00 H 31352478.00 96194888.00 23543394.00 65562312.00 46380.00 35409204.00 21803616.00 9524046.00 21770024.00 45278.00 44271.00 44271.00 Load time: 12/14/2011 04:18:54 PM Read time: 12/14/2011 04:18:54 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2065 Comment: p3_e2_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 93969264 cps Min counts: 20852 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 61660868.00 93969264.00 52728684.00 82749688.00 51013048.00 48284160.00 98445.00 82174.00 91621.00 46247.00 44194.00 44784.00 B 27792258.00 59573644.00 29768256.00 50662196.00 52396196.00 37457692.00 156212.00 130798.00 154558.00 43225.00 44537.00 41704.00 C 62903800.00 42844976.00 36098056.00 1221378.00 58112100.00 50104040.00 312159.00 211071.00 289938.00 45107.00 48053.00 48224.00 D 34166444.00 88375912.00 79666000.00 58644796.00 31591640.00 25178322.00 665414.00 488178.00 592402.00 48623.00 47426.00 48585.00 E 58721756.00 38691440.00 882416.00 55852668.00 14319957.00 34719656.00 1523177.00 303510.00 1529716.00 48471.00 48966.00 46913.00 F 35194248.00 37822892.00 30639468.00 71389824.00 67858240.00 17129676.00 4958033.00 3201114.00 4994965.00 46399.00 46590.00 47559.00 G 27346838.00 723295.00 38155600.00 80346712.00 60846496.00 28640282.00 12331275.00 3914811.00 13660751.00 48814.00 45563.00 45259.00 H 32838530.00 35670548.00 68691592.00 45182116.00 90683656.00 84750216.00 24840614.00 46837.00 23536988.00 46590.00 47160.00 46152.00 Load time: 12/14/2011 04:21:35 PM Read time: 12/14/2011 04:21:35 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2066 Comment: p3_e1_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 111335552 cps Min counts: 21080 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 102255232.00 80095440.00 81942368.00 100588048.00 72229600.00 80114400.00 90899.00 77383.00 94757.00 42161.00 42617.00 51969.00 B 88507520.00 75798960.00 59911492.00 60374204.00 59214352.00 67950024.00 169404.00 116997.00 151232.00 43700.00 43092.00 43282.00 C 91447336.00 54273152.00 53946000.00 3112469.00 98867536.00 94773376.00 286896.00 189762.00 285090.00 44194.00 43757.00 47426.00 D 39842988.00 77814824.00 96802448.00 90180544.00 88529648.00 91377568.00 668228.00 491429.00 698603.00 46666.00 46456.00 44917.00 E 84077552.00 90986744.00 1289390.00 92383752.00 44951776.00 76660960.00 2078090.00 1384148.00 1648887.00 46970.00 49308.00 42256.00 F 66172456.00 62220640.00 96956752.00 92155216.00 104942816.00 89780192.00 6291254.00 4476683.00 5466053.00 46057.00 46152.00 42883.00 G 68923880.00 1177278.00 60316676.00 89597320.00 81165024.00 70025432.00 12718243.00 12576573.00 14299338.00 45943.00 44879.00 44213.00 H 85179504.00 95947136.00 111335552.00 90212928.00 103785944.00 89644776.00 24803184.00 50905.00 25079780.00 44061.00 52615.00 48376.00 Load time: 12/14/2011 04:24:16 PM Read time: 12/14/2011 04:24:16 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2067 Comment: p3_e2_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 102257912 cps Min counts: 912 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 82421.00 81622.00 94529.00 81565.00 42693.00 44271.00 80357496.00 62551560.00 29509776.00 10090143.00 1995.00 2109.00 B 125019.00 115477.00 145567.00 47350.00 47331.00 44575.00 61192724.00 52933376.00 12398333.00 15449353.00 2071.00 2243.00 C 256463.00 195218.00 253802.00 45506.00 44784.00 46342.00 41948856.00 36834620.00 3818045.00 9871237.00 1938.00 2052.00 D 565790.00 408684.00 575218.00 46266.00 42047.00 46951.00 32871808.00 21762658.00 29050198.00 8184141.00 2224.00 2128.00 E 1373465.00 1069214.00 1364227.00 47559.00 49156.00 48452.00 53327476.00 56175448.00 23640396.00 20949260.00 2052.00 2147.00 F 3685024.00 2808383.00 4341554.00 46970.00 47749.00 46704.00 67238800.00 68007000.00 28746554.00 18434404.00 2033.00 1824.00 G 8032764.00 9083760.00 11361592.00 44537.00 45791.00 46209.00 30239862.00 89837176.00 36014236.00 14312322.00 2185.00 2071.00 H 19964250.00 42541.00 20921506.00 43320.00 46514.00 45221.00 71409472.00 102257912.00 45881020.00 14681740.00 1900.00 2090.00 Load time: 12/14/2011 04:26:47 PM Read time: 12/14/2011 04:26:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2068 Comment: p3_e1_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 123435120 cps Min counts: 931 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 93883.00 75121.00 99928.00 45867.00 55353.00 45126.00 81253120.00 83132256.00 37772796.00 8871243.00 2204.00 2204.00 B 135512.00 105402.00 141709.00 61511.00 45088.00 43529.00 91337912.00 88408016.00 21490310.00 10023337.00 1919.00 2718.00 C 249924.00 193963.00 250438.00 45487.00 44936.00 44860.00 76605152.00 92098520.00 10164317.00 12679202.00 2147.00 2185.00 D 526918.00 381882.00 543417.00 43814.00 46571.00 47198.00 43329964.00 51070268.00 98689584.00 8683893.00 2052.00 2014.00 E 868958.00 441987.00 1067047.00 46609.00 49498.00 49479.00 106088184.00 76852408.00 58068328.00 58802920.00 1919.00 2090.00 F 4029849.00 3066690.00 4013034.00 45411.00 48129.00 50600.00 110407232.00 79804824.00 99923224.00 17325372.00 2033.00 2033.00 G 10615877.00 4998884.00 9660714.00 44936.00 48566.00 49441.00 96306736.00 95246408.00 91272592.00 16670844.00 2071.00 2109.00 H 21693532.00 45772.00 21597134.00 45145.00 44765.00 45525.00 105752440.00 123435120.00 91269384.00 8223559.00 1976.00 1862.00 Load time: 12/14/2011 04:29:31 PM Read time: 12/14/2011 04:29:31 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2069 Comment: p2_e1_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 128306864 cps Min counts: 20814 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 85937.00 67955.00 87933.00 42370.00 42807.00 42826.00 92781168.00 81385616.00 80013136.00 98209080.00 51230556.00 106510496.00 B 121027.00 118537.00 122985.00 41628.00 47217.00 43301.00 69810080.00 72545248.00 84654384.00 75173960.00 75310664.00 73517936.00 C 208999.00 140321.00 205596.00 45392.00 43890.00 44765.00 82914472.00 74846600.00 73797360.00 96843392.00 109171848.00 64670312.00 D 457384.00 312121.00 405111.00 47198.00 46019.00 46514.00 71764232.00 116893384.00 91804240.00 104458480.00 80299472.00 50319708.00 E 1275723.00 468276.00 1122552.00 46609.00 44366.00 47141.00 100733808.00 77696008.00 81540744.00 41681172.00 91597960.00 56040404.00 F 5015284.00 2605753.00 3655734.00 45525.00 44480.00 47540.00 128306864.00 112438528.00 86158864.00 68833704.00 80726584.00 85341960.00 G 9685230.00 9671119.00 10658005.00 44898.00 46133.00 43909.00 117920208.00 80058384.00 98680840.00 99310176.00 108570520.00 80703152.00 H 20104312.00 21530722.00 24239864.00 42351.00 44023.00 41628.00 78797656.00 75495408.00 87725688.00 98762192.00 80703152.00 98466176.00 Load time: 12/14/2011 04:31:49 PM Read time: 12/14/2011 04:31:49 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2070 Comment: p2_e1_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 117925568 cps Min counts: 21755 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 88588072.00 99058736.00 76362472.00 39473532.00 61642312.00 63835744.00 92229.00 75521.00 84303.00 45601.00 44004.00 43510.00 B 86284896.00 76697968.00 95419160.00 79111504.00 111730184.00 54739048.00 140169.00 108386.00 123251.00 79645.00 47901.00 44080.00 C 46731676.00 61246640.00 84266832.00 86748264.00 60625244.00 46768732.00 213181.00 173073.00 207839.00 47331.00 46704.00 44955.00 D 74035256.00 81823528.00 93155272.00 87954608.00 84337120.00 114330656.00 412011.00 350518.00 451263.00 50410.00 49859.00 46000.00 E 49513620.00 77549752.00 72835696.00 84008600.00 100032352.00 66014236.00 1063188.00 809746.00 1100768.00 49745.00 47996.00 45696.00 F 66436144.00 66841664.00 79235496.00 78558352.00 117925568.00 84610224.00 5817549.00 2743811.00 4283404.00 47369.00 46761.00 46399.00 G 71321824.00 79976144.00 98509776.00 95806632.00 69556240.00 74126888.00 12363349.00 11643626.00 14390119.00 47654.00 45601.00 46057.00 H 50510020.00 104576336.00 37451200.00 85976192.00 104959800.00 71040696.00 26415328.00 24006530.00 26551530.00 46247.00 47407.00 45088.00 Load time: 12/14/2011 04:34:02 PM Read time: 12/14/2011 04:34:02 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2071 Comment: p1_e1_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 150963024 cps Min counts: 22743 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 150963024.00 117866648.00 130620864.00 92746912.00 86646904.00 88177736.00 77992.00 71700.00 97285.00 56417.00 47160.00 46989.00 B 103701624.00 116609768.00 109528136.00 66582152.00 106560064.00 85977136.00 115610.00 99053.00 129087.00 49061.00 48757.00 46742.00 C 116543440.00 125603632.00 125320592.00 84880496.00 128884592.00 98138512.00 159919.00 123156.00 206889.00 49498.00 51399.00 51304.00 D 116637712.00 114184784.00 102910744.00 114723176.00 66792348.00 130688824.00 395929.00 307616.00 380704.00 50391.00 48757.00 47179.00 E 128658816.00 123175408.00 113732768.00 92594696.00 76613432.00 83564096.00 998388.00 657031.00 865233.00 51855.00 51361.00 49232.00 F 135228528.00 120258832.00 132621072.00 122485568.00 98842440.00 112803424.00 3683254.00 2087005.00 3558783.00 49289.00 50562.00 46456.00 G 122953656.00 122508696.00 127803504.00 66006396.00 89549920.00 105548864.00 9517026.00 9775923.00 11458558.00 49821.00 49061.00 48129.00 H 142591376.00 123654688.00 118541400.00 91664816.00 98610848.00 108404240.00 11869483.00 21211280.00 23708786.00 45848.00 47464.00 45487.00 Load time: 12/14/2011 04:36:26 PM Read time: 12/14/2011 04:36:26 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2072 Comment: p1_e1_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 137054304 cps Min counts: 23599 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 80463.00 70845.00 88237.00 48833.00 48966.00 47920.00 110227480.00 100567264.00 122279728.00 71497344.00 118765616.00 137054304.00 B 103178.00 101771.00 115249.00 47559.00 49289.00 47654.00 86511152.00 76709304.00 100731200.00 98687080.00 108166288.00 122614808.00 C 174764.00 208353.00 184725.00 47274.00 48814.00 49080.00 98589616.00 105981320.00 100380552.00 90994192.00 80796112.00 106785136.00 D 364698.00 272450.00 394333.00 51874.00 48129.00 50277.00 113318936.00 111565288.00 107390488.00 106507592.00 121200464.00 99421768.00 E 733465.00 529218.00 855291.00 49403.00 52710.00 50144.00 116905656.00 95483528.00 92250208.00 80526208.00 100256464.00 130651552.00 F 1817754.00 1844374.00 3087450.00 51532.00 51171.00 49460.00 117795328.00 105758184.00 116543440.00 117046272.00 109335880.00 110658304.00 G 6867293.00 8035135.00 11297184.00 52824.00 52995.00 49061.00 116534720.00 119256784.00 107832216.00 93434752.00 92562768.00 116884608.00 H 18210160.00 20142280.00 24272662.00 47198.00 51494.00 50752.00 82508536.00 102299560.00 117585408.00 60838888.00 105606136.00 104181736.00 Load time: 12/14/2011 04:38:54 PM Read time: 12/14/2011 04:38:54 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2073 Comment: p1_e2_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 114453344 cps Min counts: 21536 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 65414136.00 103391776.00 57571968.00 55472244.00 41435836.00 44698360.00 87648.00 71605.00 94567.00 47407.00 45335.00 46837.00 B 71149104.00 74427320.00 94570784.00 31507008.00 52158912.00 50593388.00 131387.00 105725.00 125095.00 48395.00 49460.00 43073.00 C 88285728.00 68955008.00 77427904.00 42263320.00 55621244.00 47667812.00 211508.00 180486.00 210139.00 53794.00 44404.00 44993.00 D 50847804.00 66435012.00 68427776.00 70292856.00 21480054.00 54057608.00 412144.00 307996.00 527849.00 48053.00 48661.00 44955.00 E 74040896.00 52755480.00 75127528.00 29647536.00 19755948.00 47862676.00 901615.00 791365.00 982934.00 49137.00 50144.00 44765.00 F 68174928.00 42783528.00 86821792.00 36359852.00 60251324.00 53975164.00 2716972.00 2496589.00 2828797.00 47597.00 48243.00 47046.00 G 80135008.00 50619372.00 114453344.00 17978180.00 39730960.00 34785268.00 10448582.00 8811955.00 10304285.00 47920.00 48871.00 58755.00 H 79081776.00 53099800.00 94771072.00 79472472.00 49861784.00 57491624.00 25465812.00 18291486.00 24667784.00 53889.00 293454.00 48034.00 Load time: 12/14/2011 04:41:23 PM Read time: 12/14/2011 04:41:23 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2074 Comment: p1_e2_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 98385304 cps Min counts: 23779 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 80482.00 335444.00 90195.00 47730.00 47996.00 50087.00 68666760.00 45559656.00 80439688.00 37428872.00 64799488.00 91560240.00 B 115020.00 100859.00 116028.00 48585.00 50068.00 48186.00 52243260.00 40724948.00 56634740.00 73238560.00 70815840.00 74652824.00 C 185143.00 171590.00 187405.00 50391.00 47711.00 47559.00 55243564.00 38756928.00 49937576.00 43293344.00 48538512.00 41852576.00 D 413531.00 350081.00 419139.00 49194.00 50581.00 49973.00 55283972.00 59908728.00 63201484.00 30307074.00 64085844.00 69696960.00 E 1067959.00 887111.00 1034922.00 48604.00 51076.00 49498.00 42670980.00 48750960.00 27472662.00 17928584.00 60059840.00 64314532.00 F 5406012.00 3479110.00 4251110.00 50790.00 50828.00 50258.00 56193696.00 56704448.00 91051648.00 26040118.00 90192032.00 65339476.00 G 11316807.00 12059691.00 12891126.00 47749.00 49802.00 53243.00 81907024.00 85132936.00 57539204.00 31766680.00 66846256.00 88242688.00 H 24915734.00 26924496.00 26185146.00 48928.00 49118.00 50581.00 63093932.00 98385304.00 72568896.00 45042796.00 81277712.00 90355312.00