Load time: 01/12/2012 11:30:02 AM Read time: 01/12/2012 11:30:02 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2123 Comment: p1_e2_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 41068316 cps Min counts: 29456 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9791177.00 21531368.00 32386682.00 7300758.00 6348709.00 10781878.00 40575100.00 41068316.00 36335240.00 62277.00 64823.00 64633.00 B 8091594.00 9745818.00 20447584.00 4271243.00 22317962.00 6924193.00 17172226.00 18619924.00 13511839.00 63474.00 79628.00 58913.00 C 13263967.00 8694434.00 11367868.00 6405401.00 6905905.00 6803363.00 6831953.00 6961402.00 3626552.00 67731.00 67465.00 62353.00 D 6495041.00 8842764.00 7635582.00 9747283.00 3068336.00 8025646.00 1544785.00 3547153.00 1343852.00 68225.00 68491.00 66002.00 E 12466680.00 6302262.00 8943509.00 3954000.00 2724362.00 6273368.00 4395037.00 1002839.00 566309.00 67332.00 65564.00 62771.00 F 7213823.00 8316341.00 8992553.00 4683515.00 7651839.00 7125027.00 591129.00 880869.00 278888.00 71760.00 68206.00 63094.00 G 9883996.00 6366545.00 16962562.00 2286975.00 6208181.00 5491819.00 196086.00 331758.00 165318.00 71076.00 70468.00 62182.00 H 10109890.00 7477402.00 12260274.00 8640850.00 7716015.00 8360969.00 132859.00 873438.00 118510.00 64823.00 64842.00 61650.00 Load time: 01/12/2012 11:32:52 AM Read time: 01/12/2012 11:32:52 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2124 Comment: p1_e2_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42116440 cps Min counts: 30245 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 41604780.00 42116440.00 40239084.00 64842.00 67275.00 65070.00 8264635.00 6701416.00 8740778.00 7687750.00 11213556.00 17583996.00 B 14992219.00 17314854.00 13694207.00 65393.00 63037.00 64120.00 8511114.00 7856878.00 7695838.00 15186727.00 11730603.00 9052478.00 C 4231771.00 5799846.00 3420110.00 65146.00 65754.00 68282.00 6394152.00 5351372.00 5994477.00 5874056.00 5826701.00 5732616.00 D 1484028.00 2188121.00 1405236.00 70144.00 69023.00 68377.00 6734865.00 6784223.00 7417671.00 3579916.00 7070290.00 9150119.00 E 792518.00 1044876.00 647496.00 66021.00 66268.00 80274.00 5362694.00 4808617.00 3031868.00 1880104.00 5938991.00 8237269.00 F 391641.00 481132.00 289587.00 67427.00 65830.00 68472.00 6502969.00 9630394.00 14378439.00 3831644.00 11758743.00 7692248.00 G 193844.00 282575.00 152072.00 62942.00 63949.00 63341.00 10150045.00 12562935.00 7785954.00 4214485.00 9268491.00 21802996.00 H 137116.00 188674.00 116895.00 63683.00 60490.00 84702.00 7433672.00 14498847.00 8346194.00 5035655.00 11051473.00 14390640.00 Load time: 01/12/2012 11:36:11 AM Read time: 01/12/2012 11:36:11 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2125 Comment: p2_e2_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42243748 cps Min counts: 30131 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4369015.00 8452583.00 6034409.00 6376266.00 3246445.00 3792601.00 40594564.00 41854880.00 42243748.00 63455.00 62049.00 60737.00 B 8138003.00 8467055.00 7031949.00 7548535.00 64006.00 3526602.00 17748132.00 20168178.00 16751967.00 66439.00 63759.00 60262.00 C 14038382.00 4799760.00 6466729.00 9383934.00 131699.00 2487294.00 5889629.00 6460176.00 4698844.00 63835.00 65431.00 64481.00 D 5421909.00 7383525.00 8338443.00 9321009.00 349071.00 5907832.00 1972828.00 2520782.00 1622258.00 67047.00 66306.00 64614.00 E 4931212.00 7688487.00 7473160.00 7432657.00 78905.00 5466212.00 836665.00 1111582.00 623417.00 67674.00 67275.00 66363.00 F 5681939.00 6743968.00 6600474.00 10525636.00 116933.00 8373083.00 386795.00 515321.00 330428.00 68890.00 69536.00 68472.00 G 3959147.00 6384246.00 7611139.00 7310467.00 1073782.00 6934345.00 243217.00 311860.00 195744.00 69555.00 100627.00 68909.00 H 4206231.00 13945828.00 2764011.00 8224902.00 155189.00 3716524.00 2165856.00 193578.00 130236.00 70753.00 105740.00 72995.00 Load time: 01/12/2012 11:39:15 AM Read time: 01/12/2012 11:39:15 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2126 Comment: p2_e2_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37832416 cps Min counts: 28354 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 33559692.00 37832416.00 33106098.00 57279.00 59103.00 56708.00 4978934.00 9141511.00 11038512.00 6948651.00 6404232.00 7655715.00 B 14077046.00 16623466.00 13988633.00 57374.00 61288.00 60452.00 5064390.00 6968812.00 5311625.00 8012082.00 6402657.00 8261706.00 C 4775002.00 5935513.00 4004017.00 61973.00 60946.00 61574.00 9232587.00 7259227.00 7158456.00 9191847.00 8763619.00 6860373.00 D 1542657.00 2375328.00 1403488.00 62847.00 61916.00 65545.00 10384668.00 9549501.00 7839699.00 5950959.00 6308652.00 5914429.00 E 747649.00 1029141.00 673741.00 61973.00 63797.00 66667.00 4160633.00 2128956.00 4805242.00 4267267.00 7596269.00 4284927.00 F 354221.00 488164.00 319177.00 64823.00 66686.00 64652.00 6775336.00 5548142.00 5141837.00 4390741.00 7926369.00 4205357.00 G 195288.00 290462.00 184113.00 62467.00 63474.00 64310.00 5664865.00 6161799.00 8340435.00 5364811.00 6627770.00 3597919.00 H 133163.00 148765.00 123547.00 61954.00 63930.00 61079.00 4265018.00 4574287.00 6252371.00 7543049.00 3377196.00 5562319.00 Load time: 01/12/2012 11:42:04 AM Read time: 01/12/2012 11:42:04 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2127 Comment: p3_e2_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 36690440 cps Min counts: 27632 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7672165.00 12603230.00 5479642.00 10175027.00 8249386.00 6004937.00 34411256.00 36690440.00 25014850.00 56879.00 58628.00 55264.00 B 3429169.00 7881866.00 4770245.00 8638290.00 6711342.00 5284200.00 18962286.00 18081276.00 16950666.00 56537.00 56328.00 56898.00 C 7266057.00 6294506.00 4239947.00 234494.00 7739768.00 6659990.00 5809173.00 6731033.00 4545172.00 59483.00 60794.00 59388.00 D 4200400.00 10757054.00 10069310.00 7432217.00 3816073.00 4107637.00 1952526.00 2639111.00 1629290.00 65488.00 64595.00 63227.00 E 7412436.00 6156025.00 167200.00 8146929.00 2019297.00 5512689.00 916293.00 1275152.00 736702.00 64595.00 59768.00 62182.00 F 5486473.00 5330262.00 7333677.00 10417774.00 10197260.00 3648724.00 407547.00 596279.00 372427.00 60338.00 66306.00 59141.00 G 6696838.00 224270.00 7274517.00 8981877.00 8045787.00 4333205.00 214938.00 317847.00 191924.00 60813.00 159883.00 59008.00 H 7133685.00 7806559.00 9158042.00 5923296.00 10410599.00 9177109.00 135329.00 170582.00 172787.00 59996.00 59160.00 115679.00 Load time: 01/12/2012 11:45:06 AM Read time: 01/12/2012 11:45:06 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2128 Comment: p3_e2_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 46428312 cps Min counts: 978 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37954712.00 46428312.00 39257688.00 56024.00 55796.00 57583.00 11565120.00 11638680.00 3073275.00 1396133.00 9255.00 9483.00 B 16026145.00 23735772.00 16434401.00 59787.00 62106.00 59255.00 9744951.00 6592224.00 1888440.00 2107917.00 43918.00 51748.00 C 5015929.00 8047500.00 5220551.00 57792.00 58115.00 59901.00 5657002.00 5338329.00 700936.00 1312343.00 6860.00 10395.00 D 1943426.00 3348755.00 2097563.00 58970.00 55701.00 62809.00 3947350.00 3144862.00 4465275.00 1332051.00 70639.00 9350.00 E 920398.00 1624171.00 897878.00 57374.00 60984.00 61079.00 6575246.00 9188320.00 4155473.00 3458481.00 6689.00 17217.00 F 416441.00 684820.00 442496.00 65488.00 64044.00 62163.00 9605664.00 7785112.00 3691264.00 2545183.00 9065.00 9483.00 G 245193.00 425791.00 258705.00 57184.00 60927.00 58400.00 4771630.00 12628256.00 5206946.00 2030733.00 8342.00 7829.00 H 160871.00 232023.00 153478.00 55625.00 55625.00 55169.00 7667562.00 15804428.00 6103714.00 2207775.00 1957.00 2337.00