Load time: 11/04/2011 02:04:55 PM Read time: 11/04/2011 02:04:55 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1994 Comment: wheat 9 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 810541 cps Min counts: 16288 counts Well list: r0(a1:h11) = cps(f2(a1:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 800024.00 583264.00 730230.00 691225.00 710509.00 695847.00 737000.00 736430.00 689590.00 615898.00 396722.00 B 776785.00 700677.00 691891.00 729679.00 780341.00 742059.00 713267.00 662832.00 647447.00 621489.00 366161.00 C 699631.00 684722.00 701096.00 554243.00 774122.00 710357.00 671581.00 675726.00 552589.00 495707.00 363289.00 D 712829.00 665704.00 696189.00 773210.00 744227.00 810541.00 596405.00 760981.00 714218.00 678731.00 157445.00 E 738712.00 716195.00 468969.00 764043.00 691378.00 783726.00 692975.00 764633.00 696265.00 708094.00 148583.00 F 596747.00 604487.00 586991.00 605951.00 647999.00 635352.00 634801.00 645451.00 709863.00 540436.00 66275.00 G 666065.00 311752.00 568696.00 653039.00 625330.00 613121.00 617590.00 705298.00 653609.00 581704.00 79587.00 H 500348.00 407847.00 468208.00 414142.00 442630.00 387955.00 471004.00 469616.00 420037.00 238002.00 32576.00 Load time: 11/04/2011 02:12:12 PM Read time: 11/04/2011 02:12:12 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1995 Comment: wheat 9 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 848975 cps Min counts: 1454 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 848975.00 617590.00 778515.00 695580.00 712620.00 701628.00 759992.00 719542.00 688373.00 604183.00 411289.00 2909.00 B 836633.00 727587.00 701818.00 760943.00 776557.00 735213.00 719447.00 705546.00 658173.00 603004.00 342085.00 2985.00 C 735612.00 722034.00 714370.00 565235.00 803485.00 753165.00 692652.00 713305.00 609051.00 524842.00 338129.00 2909.00 D 762883.00 693964.00 707923.00 790610.00 779523.00 821666.00 598991.00 734604.00 706972.00 677495.00 165489.00 16925.00 E 789622.00 742116.00 452024.00 766801.00 717945.00 786959.00 676221.00 764880.00 682439.00 688316.00 166383.00 3004.00 F 637710.00 632443.00 608975.00 621774.00 658192.00 629248.00 631910.00 662756.00 712677.00 530585.00 94440.00 3042.00 G 708722.00 335315.00 566300.00 669070.00 636798.00 620671.00 625939.00 709083.00 647390.00 569172.00 50205.00 2966.00 H 523777.00 436297.00 481292.00 430364.00 461324.00 417451.00 482966.00 481330.00 412069.00 233210.00 39822.00 7854.00