Load time: 05/31/2011 08:12:40 AM Read time: 05/31/2011 08:12:40 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1565 Comment: 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 80887816 cps Min counts: 14622 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 71762992.00 80887816.00 80673472.00 32383.00 B 54340500.00 63113120.00 69588816.00 29453.00 C 41460312.00 41157716.00 43504132.00 30652.00 D 23335392.00 22939824.00 16110646.00 174610.00 E 14479042.00 12423343.00 12463973.00 29244.00 F 7888094.00 7401422.00 6736282.00 29586.00 G 4999141.00 5208307.00 5277544.00 476797.00 H 3629734.00 3740398.00 3719416.00 175904.00