Load time: 05/31/2011 08:15:35 AM Read time: 05/31/2011 08:15:35 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1566 Comment: 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 53938460 cps Min counts: 20492 counts Well list: r0(a1:g12) = cps(f2(a1:g12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 29079316.00 35311980.00 5821780.00 26666462.00 30495976.00 38341568.00 37985380.00 7115568.00 44511168.00 9655038.00 31703288.00 43809084.00 B 41426592.00 49653796.00 38740092.00 29076278.00 46015224.00 30481902.00 35702160.00 16104852.00 32354926.00 27403650.00 34652852.00 53938460.00 C 37526476.00 20608188.00 39885056.00 29711558.00 30603876.00 24888184.00 28193150.00 9787976.00 23427778.00 10485891.00 16555554.00 26408128.00 D 31618526.00 14698514.00 35901988.00 10864147.00 16433787.00 17724456.00 10761713.00 9628843.00 18586880.00 25997526.00 26164406.00 12035657.00 E 13433388.00 7783677.00 25048406.00 8564017.00 37532620.00 10177792.00 12063060.00 4708055.00 20388278.00 14053718.00 11787414.00 6021645.00 F 22715166.00 5473001.00 15756269.00 6578731.00 20552808.00 28623382.00 19624012.00 4941039.00 9207764.00 12796283.00 14707166.00 4413090.00 G 17901762.00 7034012.00 29763870.00 6501182.00 13861472.00 29715750.00 18890078.00 4793922.00 66423.00 56377.00 48100.00 40984.00 H