Load time: 10/29/2010 02:18:48 PM Read time: 10/29/2010 02:18:48 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1123 Comment: dna Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 46869400 cps Min counts: 29941 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13859573.00 11922277.00 14415100.00 3933617.00 12154172.00 10967522.00 11306062.00 13776162.00 11740057.00 8390365.00 11525871.00 7764701.00 B 10926008.00 11039781.00 9701988.00 7839473.00 13514748.00 13048335.00 682120.00 12254427.00 14289290.00 11247151.00 9160649.00 14332580.00 C 16027284.00 9483431.00 6957745.00 12881175.00 12544729.00 12594439.00 11495231.00 16714769.00 14742853.00 14435624.00 9028649.00 7375722.00 D 46869400.00 19220100.00 34290088.00 22381780.00 18269822.00 21064208.00 59882.00 18619662.00 12373452.00 16391987.00 10924920.00 10765099.00 E 33618076.00 23240220.00 23779210.00 32428546.00 19041580.00 19711870.00 22823452.00 21239918.00 24428990.00 10393332.00 19504750.00 15030599.00 F 12036615.00 14898533.00 38050260.00 23284086.00 15164613.00 12794590.00 30834146.00 29397708.00 12206674.00 9137877.00 21506620.00 23981448.00 G 9519115.00 8648050.00 13446524.00 9111967.00 6543386.00 19406824.00 23682346.00 2244658.00 18434448.00 11020634.00 19836700.00 7459901.00 H 10547277.00 8883373.00 8272529.00 10308667.00 15908345.00 17147380.00 9931425.00 18315058.00 15291434.00 12775511.00 8075371.00 6977110.00