Load time: 10/29/2010 02:21:22 PM Read time: 10/29/2010 02:21:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1124 Comment: dna Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 23010986 cps Min counts: 30026 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7643454.00 8053174.00 6623383.00 15613182.00 7637298.00 15505036.00 4346300.00 16192823.00 8793340.00 8540958.00 863710.00 8096882.00 B 4942907.00 9952750.00 13454855.00 7374592.00 62014.00 5075087.00 18445482.00 23010986.00 6116921.00 15456653.00 6291228.00 13840185.00 C 1783429.00 12921095.00 17052122.00 19232424.00 14874572.00 9379033.00 60053.00 21348384.00 17140292.00 16059772.00 12051928.00 8185693.00 D 6565791.00 4389539.00 10467019.00 15488126.00 12895300.00 11695921.00 5073918.00 6224208.00 6231247.00 5427435.00 16796582.00 7278253.00 E 1051638.00 6827771.00 5267360.00 13315646.00 19114782.00 15299326.00 17590074.00 12552889.00 11493705.00 3993832.00 2645209.00 14320575.00 F 5474097.00 19609046.00 21924034.00 4219971.00 19676250.00 18708890.00 20187300.00 7882184.00 16907790.00 10690394.00 15575784.00 12990158.00 G 12272116.00 17695794.00 17989032.00 16006258.00 13421571.00 16687650.00 11925558.00 20396702.00 21865314.00 65231.00 3044561.00 2106602.00 H 3601705.00 4048275.00 1607392.00 8591140.00 20093858.00 11589038.00 16384510.00 10714591.00 17731412.00 6896642.00 14635420.00 8558708.00