Load time: 11/11/2010 09:48:59 AM Read time: 11/11/2010 09:48:59 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1149 Comment: T12_1-24_triplicates Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 78153208 cps Min counts: 13464 counts Well list: r0(a1:h7,a8,b8) = cps(f2(a1:h7,a8,b8)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 78153208.00 73569552.00 77311632.00 32140.00 20603756.00 18173804.00 18357626.00 13704796.00 B 48438692.00 45483960.00 47627512.00 33357.00 90905.00 17371854.00 5495130.00 13266422.00 C 28589546.00 27205832.00 26984084.00 29534.00 15713186.00 37783944.00 2597221.00 D 16663454.00 16002572.00 15525160.00 28850.00 213457.00 828651.00 12513095.00 E 9736147.00 9413738.00 9041989.00 156384.00 14310319.00 2166433.00 20035094.00 F 5876562.00 5910772.00 5682342.00 26929.00 15952662.00 14018285.00 24057632.00 G 3582988.00 3532398.00 3467149.00 29116.00 15947829.00 44495904.00 41394844.00 H 2214947.00 2225477.00 2295937.00 30029.00 13451758.00 17324372.00 9704743.00