Load time: 04/08/2011 02:06:12 PM Read time: 04/08/2011 02:06:12 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1438 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37665532 cps Min counts: 31677 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 440524.00 13182507.00 543493.00 22675008.00 14037381.00 18218684.00 25814350.00 12218417.00 94415.00 15871943.00 9761597.00 17550556.00 B 642471.00 17848358.00 24018378.00 37665532.00 636464.00 20484512.00 319344.00 2627335.00 23959250.00 21443668.00 1585286.00 15314098.00 C 876048.00 2245564.00 25321538.00 24859886.00 16674450.00 24184524.00 256292.00 19059260.00 11815320.00 24068494.00 772965.00 80387.00 D 352761.00 3252199.00 21758644.00 27351448.00 35139924.00 1090237.00 12026125.00 26705076.00 12842899.00 1452921.00 14064437.00 63355.00 E 13612076.00 24033800.00 27193348.00 24556762.00 14012622.00 155585.00 21337652.00 27559016.00 305087.00 22889994.00 11173420.00 151137.00 F 15804380.00 309611.00 19648458.00 17349306.00 12771944.00 11380674.00 380000.00 12522022.00 11460379.00 10196117.00 11476866.00 25099994.00 G 4108214.00 25846056.00 14361637.00 19441658.00 13013009.00 21908402.00 24008232.00 17304076.00 10425957.00 2015787.00 1417584.00 16379199.00 H 11129878.00 21461698.00 10056857.00 15307596.00 2165106.00 22222684.00 4945329.00 12360895.00 2367197.00 19131712.00 6701276.00 13016272.00