Load time: 04/08/2011 02:01:28 PM Read time: 04/08/2011 02:01:28 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1436 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 95668896 cps Min counts: 17468 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 93854864.00 91831312.00 95668896.00 37237.00 B 47335700.00 52198080.00 56382104.00 35222.00 C 25403330.00 25570776.00 28131262.00 34937.00 D 15804414.00 15937243.00 16124119.00 36287.00 E 9230850.00 8985655.00 9543461.00 37313.00 F 5006866.00 5264049.00 5546560.00 37770.00 G 2999513.00 3076852.00 3411494.00 37751.00 H 2006021.00 2004220.00 2161366.00 35964.00