Load time: 05/24/2011 08:16:59 AM Read time: 05/24/2011 08:16:59 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1559 Comment: 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 50298184 cps Min counts: 15153 counts Well list: r0(a1:h4) = cps(f2(a1:h4)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 50298184.00 48070464.00 49367132.00 65504.00 B 27267474.00 27440206.00 27023706.00 32286.00 C 14912650.00 15565052.00 14733751.00 31487.00 D 8580629.00 8657511.00 8371710.00 31144.00 E 5141193.00 4980220.00 4759190.00 30307.00 F 2953398.00 2948762.00 2948112.00 31334.00 G 1699498.00 1808887.00 1821161.00 57361.00 H 1064071.00 1038672.00 1049269.00 91378.00