Load time: 05/24/2011 08:19:30 AM Read time: 05/24/2011 08:19:30 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #1560 Comment: 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 18532706 cps Min counts: 779279 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10764843.00 6267749.00 9985557.00 6921057.00 9188278.00 8627196.00 5772726.00 4903561.00 8035694.00 4673522.00 5828017.00 6581039.00 B 11993498.00 10664504.00 11292275.00 9084359.00 11325492.00 9405481.00 14722450.00 7485087.00 13374935.00 5083564.00 5937827.00 9433126.00 C 12884684.00 9148002.00 11861204.00 13735555.00 13522614.00 11535710.00 13262082.00 9520085.00 13783515.00 6340361.00 8687538.00 7218154.00 D 11264445.00 9146908.00 13140273.00 10938489.00 16934794.00 12887433.00 11397815.00 12880617.00 12085363.00 8712196.00 10205115.00 11749663.00 E 9494984.00 5139287.00 11060361.00 8800500.00 18309718.00 12864585.00 13105357.00 9578463.00 10635294.00 9162653.00 5915744.00 11200944.00 F 9997805.00 4167223.00 5023855.00 7506927.00 14333825.00 13331598.00 11271140.00 13937738.00 13908212.00 6715330.00 6282746.00 14788106.00 G 9582198.00 12637334.00 4695503.00 9079828.00 15083966.00 16835536.00 10924949.00 16750099.00 14420401.00 10089972.00 13494094.00 18532706.00 H 9848942.00 8634163.00 5865063.00 12626646.00 12766981.00 16360495.00 11889599.00 14346201.00 12751542.00 10061781.00 4576523.00 12705347.00