Load time: 09/05/2012 02:25:13 PM Read time: 09/05/2012 02:25:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2887 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 135800736 cps Min counts: 13168 counts Well list: r0(a2:h5,a7:h10) = cps(f2(a2:h5,a7:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 117139496.00 72093736.00 30486222.00 37407.00 135800736.00 103219512.00 61891916.00 26336.00 B 74707912.00 45741588.00 73867856.00 33232.00 65205044.00 85435256.00 86207136.00 27111.00 C 47354420.00 32061.00 50327912.00 33818.00 55450248.00 46903232.00 35301624.00 27092.00 D 31227556.00 25199600.00 23115298.00 31532.00 31255812.00 24130310.00 33213.00 28358.00 E 16952670.00 17318560.00 12266346.00 31796.00 13112939.00 17918226.00 17349584.00 27243.00 F 11038447.00 13179738.00 9096581.00 32117.00 30247.00 9325368.00 10637993.00 28055.00 G 5947183.00 5090043.00 3837036.00 38900.00 6361116.00 6892701.00 6228782.00 29491.00 H 3205310.00 35650.00 2854867.00 36274.00 3444587.00 3003902.00 3641524.00 32816.00 Load time: 09/05/2012 02:30:41 PM Read time: 09/05/2012 02:30:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2888 Comment: setaflat1apex Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 44039640 cps Min counts: 18807 counts Well list: r0(a1:h11) = cps(f2(a1:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7295674.00 44511.00 10965945.00 6784126.00 11813556.00 3764892.00 37615.00 8600613.00 5010012.00 2906971.00 1006832.00 B 8116044.00 12155250.00 41960.00 6321401.00 31016158.00 4931575.00 4443989.00 13657851.00 11577032.00 1749756.00 8770683.00 C 11255515.00 57679.00 6424798.00 45115.00 3859557.00 42584.00 60891.00 9332893.00 6913224.00 66124.00 5036092.00 D 39353.00 45607.00 44039640.00 47420.00 74191.00 14214963.00 3196167.00 4062240.00 1129918.00 11051499.00 3247622.00 E 81843.00 12295815.00 59682.00 3541227.00 75797.00 3008661.00 44227.00 4972229.00 1353174.00 20275288.00 2548209.00 F 67220.00 7563229.00 4512351.00 43434.00 76912.00 9232148.00 7886362.00 3550993.00 3588830.00 5715772.00 15861550.00 G 3619918.00 47987.00 66729.00 43434.00 91554.00 7564649.00 5644258.00 3916214.00 9229923.00 4405002.00 11316564.00 H 2198974.00 47685.00 633303.00 670390.00 195313.00 6793155.00 50046.00 90647.00 1758836.00 1899573.00 1594373.00