Load time: 02/14/2013 09:54:16 AM Read time: 02/14/2013 09:54:16 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2949 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 125754968 cps Min counts: 1141 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2283.00 93701640.00 123487288.00 124641952.00 39997.00 11029832.00 25147474.00 6742105.00 5722125.00 6796138.00 11613650.00 10365300.00 B 2680.00 118738448.00 121265696.00 125754968.00 46301.00 10735484.00 26016024.00 15192973.00 14729369.00 9704507.00 12993342.00 10333838.00 C 2491.00 76531240.00 76488088.00 77290192.00 48453.00 12856444.00 20957744.00 13691361.00 16389458.00 10348804.00 12384155.00 11715881.00 D 2529.00 45862268.00 42170288.00 46715220.00 47339.00 12745135.00 19838082.00 12710458.00 12707888.00 12112265.00 13058853.00 39128.00 E 2548.00 27593374.00 27083868.00 27347900.00 48566.00 13368012.00 21424142.00 20814710.00 19445356.00 12614832.00 17218880.00 39657.00 F 2453.00 16790342.00 16688033.00 16711805.00 45320.00 16014500.00 18990554.00 3916052.00 19611882.00 15665386.00 20292322.00 37939.00 G 2378.00 9868057.00 9656002.00 9851774.00 46414.00 15399845.00 20549432.00 4557126.00 17543090.00 11621803.00 15190108.00 38317.00 H 2472.00 6735181.00 6287595.00 6297192.00 53700.00 15674435.00 20560252.00 18473448.00 19873470.00 15314010.00 17800500.00 36297.00 Load time: 02/14/2013 09:56:23 AM Read time: 02/14/2013 09:56:23 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2950 Comment: read2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 126609608 cps Min counts: 1151 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 2434.00 90726208.00 119546264.00 124081840.00 34334.00 10538619.00 24433336.00 6578836.00 5665390.00 6620247.00 11524479.00 9898658.00 B 2434.00 118080144.00 119503768.00 126609608.00 39110.00 10432718.00 25435636.00 14729789.00 14069963.00 9317484.00 13120528.00 10480433.00 C 2378.00 76185096.00 76386824.00 76422304.00 42488.00 12553078.00 20337374.00 13184767.00 15684993.00 10188466.00 12314898.00 11620282.00 D 2472.00 45765900.00 41672504.00 45970252.00 41488.00 12570160.00 19325590.00 12638402.00 12587351.00 11962025.00 12813383.00 34863.00 E 2302.00 26845752.00 26759246.00 27031504.00 44055.00 13206907.00 21302480.00 20413572.00 19153098.00 12553546.00 16769947.00 34844.00 F 2434.00 16418485.00 16574482.00 16645493.00 40827.00 15529327.00 18780752.00 3849129.00 19363490.00 15322539.00 19699732.00 33334.00 G 2434.00 9607299.00 9294444.00 9705518.00 41092.00 14933672.00 20150688.00 4480954.00 17189748.00 11411582.00 14746393.00 33032.00 H 2359.00 6486957.00 6127830.00 6225509.00 45735.00 15378250.00 20477804.00 18550618.00 19541146.00 14868707.00 16928390.00 32447.00