Load time: 03/26/2012 01:48:20 PM Read time: 03/26/2012 01:48:20 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2407 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 79578352 cps Min counts: 16580 counts Well list: r0(a2:h5) = cps(f2(a2:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37669868.00 79578352.00 41116348.00 34681.00 B 26741338.00 57029312.00 37434480.00 49493.00 C 22529648.00 43779652.00 39301656.00 34548.00 D 23560090.00 28435530.00 27698386.00 35670.00 E 17633726.00 22914718.00 23328542.00 36259.00 F 13742668.00 12298707.00 15388996.00 35803.00 G 7647622.00 5577501.00 6198357.00 41659.00 H 4041974.00 4135788.00 3592622.00 33160.00 Load time: 03/26/2012 01:53:13 PM Read time: 03/26/2012 01:53:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2408 Comment: standards 2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 78497936 cps Min counts: 15344 counts Well list: r0(a2:h5) = cps(f2(a2:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 49709864.00 78497936.00 42982072.00 31410.00 B 34126124.00 69421888.00 60354108.00 48808.00 C 28357568.00 44413044.00 57473312.00 30688.00 D 26234200.00 29939006.00 31091934.00 31753.00 E 17538658.00 23974506.00 26225454.00 32760.00 F 12855628.00 13293688.00 16528038.00 31867.00 G 8850097.00 7969333.00 5375054.00 39834.00 H 4837466.00 4788376.00 3562768.00 32076.00 Load time: 03/26/2012 01:56:36 PM Read time: 03/26/2012 01:56:36 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2409 Comment: rep3 plate 1 samples 1-88 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31586628 cps Min counts: 502841 counts Well list: r0(a1:h11) = cps(f2(a1:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4186125.00 9521537.00 5323569.00 20554050.00 18644202.00 17115936.00 14652524.00 1205348.00 1005683.00 11447928.00 14885074.00 B 6673562.00 16419527.00 5163888.00 26835600.00 23794460.00 18449296.00 18421020.00 11674308.00 4873498.00 13096188.00 2880005.00 C 6232863.00 9283078.00 9350989.00 31586628.00 21626686.00 12581796.00 21549848.00 7539647.00 7667196.00 10472457.00 7690744.00 D 4937817.00 13573073.00 6827816.00 16976734.00 14106770.00 16226783.00 22359148.00 10541017.00 11444210.00 13324733.00 12904280.00 E 8936014.00 12715442.00 5468975.00 17115110.00 9466034.00 12434847.00 16386293.00 14133394.00 12035306.00 6496899.00 9999980.00 F 8490431.00 13698676.00 1215920.00 14193616.00 12757328.00 14243430.00 11033560.00 4462659.00 15111098.00 11506806.00 10150554.00 G 8981782.00 4785364.00 9366747.00 11938099.00 9591000.00 11039817.00 2243870.00 14768525.00 16019906.00 2359704.00 6951899.00 H 6018605.00 19284620.00 4158942.00 9794386.00 18156688.00 3438641.00 1137887.00 10133135.00 10443065.00 4718744.00 11623918.00 Load time: 03/26/2012 01:58:44 PM Read time: 03/26/2012 01:58:44 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2410 Comment: rep3 plate 1 samples 1-88 read2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31891754 cps Min counts: 529946 counts Well list: r0(a1:h11) = cps(f2(a1:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4387055.00 8559126.00 6086550.00 20693534.00 18532756.00 16308845.00 13298342.00 1181200.00 1059892.00 11006988.00 14703145.00 B 8036510.00 15893000.00 5912325.00 28163750.00 24090860.00 16076089.00 18608566.00 13755994.00 6619720.00 13033115.00 2816960.00 C 6934795.00 13177802.00 10178040.00 31891754.00 21767706.00 11310025.00 23408968.00 10110049.00 8123886.00 11783056.00 7797068.00 D 5789629.00 23087396.00 7777539.00 17974198.00 12929257.00 16393881.00 23592922.00 9793426.00 13549696.00 14056105.00 13111865.00 E 9437289.00 15632778.00 6632223.00 17316494.00 9953586.00 12526892.00 15308503.00 14507830.00 11464662.00 7739498.00 10202922.00 F 9160782.00 14028102.00 1537198.00 14162179.00 14772560.00 15087737.00 11069851.00 4640833.00 16282488.00 13071702.00 10508064.00 G 8884980.00 5304793.00 9963850.00 11746802.00 10845025.00 12992889.00 2563876.00 14359718.00 13763288.00 2403851.00 8695456.00 H 6567985.00 16972738.00 4280204.00 10311123.00 17611078.00 3383497.00 1253168.00 9930323.00 10629540.00 4946210.00 10920833.00 Load time: 03/26/2012 02:01:30 PM Read time: 03/26/2012 02:01:30 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2411 Comment: rep3 plate2 samples 89-168 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 25707776 cps Min counts: 104443 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 457208.00 10635841.00 13214875.00 11057435.00 14599072.00 18275776.00 11107442.00 5946363.00 208886.00 11681084.00 B 11908456.00 11335804.00 11667556.00 8973006.00 14730982.00 12417637.00 24601178.00 20218312.00 25707776.00 10227280.00 C 8096632.00 10065127.00 18000744.00 18457738.00 13983947.00 10564296.00 13841415.00 8969720.00 16977438.00 14111882.00 D 11855252.00 13181616.00 11956427.00 12626304.00 16450117.00 14893158.00 2851145.00 12536551.00 19916260.00 14517810.00 E 14726831.00 7376968.00 9188840.00 9988807.00 7717609.00 12518051.00 16738761.00 15349870.00 15899618.00 13335755.00 F 16282865.00 13885960.00 4817350.00 13229285.00 10954612.00 12924795.00 6271213.00 16964236.00 15023010.00 15313741.00 G 13051198.00 10401170.00 8583879.00 9239730.00 5310558.00 13623504.00 5632714.00 13956658.00 14832544.00 10138661.00 H 8831129.00 15682294.00 5226579.00 12155355.00 7749131.00 9731419.00 7486550.00 13458843.00 13615643.00 19937980.00 Load time: 03/26/2012 02:04:00 PM Read time: 03/26/2012 02:04:00 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2412 Comment: rep5 plate1 samples 1-88 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 21478590 cps Min counts: 778809 counts Well list: r0(a1:h11) = cps(f2(a1:h11)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7231439.00 9426289.00 2348873.00 2183139.00 9230005.00 9492166.00 2776747.00 8724445.00 6275240.00 9185069.00 17496970.00 B 18339382.00 10133372.00 4069771.00 5079382.00 14654314.00 5067941.00 6356226.00 10113160.00 10467010.00 7213215.00 5126947.00 C 16160921.00 7320201.00 3573662.00 11188792.00 11694994.00 11062084.00 13287383.00 11471044.00 10026616.00 8028905.00 9641534.00 D 21478590.00 6586872.00 8616073.00 7202282.00 13976645.00 15977608.00 13646085.00 5507885.00 4790259.00 7362365.00 5444472.00 E 18764548.00 8165127.00 3468336.00 5586507.00 11803951.00 12381353.00 9093979.00 13394538.00 8030238.00 9112185.00 7936013.00 F 13198258.00 12238388.00 1661855.00 12607744.00 13090246.00 18536636.00 2725543.00 9581431.00 5694620.00 7739767.00 4400684.00 G 18420234.00 7065991.00 7263583.00 9164307.00 8878772.00 14078261.00 4933610.00 6025991.00 8296821.00 5456522.00 11076259.00 H 6511321.00 10656709.00 4644730.00 10881071.00 15237309.00 8880309.00 3373338.00 7098062.00 4318243.00 8403254.00 6415234.00 Load time: 03/26/2012 02:06:27 PM Read time: 03/26/2012 02:06:27 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2413 Comment: rep5 plate2 samples 89-168 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 18960528 cps Min counts: 532151 counts Well list: r0(a1:h10) = cps(f2(a1:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 7707397.00 8363736.00 10740643.00 12932630.00 6888929.00 12139496.00 6555210.00 10616572.00 6033624.00 12269333.00 B 15277782.00 10674354.00 11992993.00 4134677.00 9333128.00 11399619.00 5854252.00 18339986.00 4675152.00 11900140.00 C 10233381.00 9661544.00 16406071.00 14405510.00 9261331.00 10761852.00 5108545.00 6093613.00 6716473.00 4832271.00 D 10453874.00 16380606.00 9438295.00 11095068.00 18960528.00 16016910.00 11480379.00 4619352.00 5067846.00 13202777.00 E 11020290.00 10665160.00 5745927.00 5855282.00 11009946.00 7649132.00 4414962.00 10162196.00 11189164.00 12273218.00 F 11922765.00 9569129.00 8422993.00 1064303.00 11507129.00 7415537.00 3225078.00 12085976.00 5632514.00 8224475.00 G 12606765.00 7679634.00 11620882.00 11446414.00 12029003.00 15971546.00 10694979.00 15661574.00 9869891.00 4829127.00 H 6568700.00 13327995.00 2396206.00 14413453.00 9207156.00 5154571.00 2870553.00 9779639.00 2433652.00 5161478.00 Load time: 03/27/2012 10:14:54 AM Read time: 03/27/2012 10:14:54 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Daves New 2 Plate ID: #2416 Comment: 31712read1 Microplate format: Packard-Optiplate 96 Flat White PS Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: l2 Light sensor: HC-124 Excitation side: Top Emission side: Top Lamp: None Readings per well: 1 Time between readings: Integration time: 1000000 us Attenuator mode: o Motion settling time: 0 ms Luminescence height: 0.5 mm Numeric Luminescence aperture: 96 Max cps: 36 cps Min counts: 7 counts Dark Count reading: 32 counts Well list: r0(a1:h12) = (l2(a1:h12)) Data: RAW DATA Units: rlu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 21.00 16.00 36.00 13.00 11.00 16.00 16.00 18.00 18.00 21.00 23.00 19.00 B 18.00 21.00 9.00 16.00 18.00 16.00 24.00 19.00 22.00 28.00 16.00 17.00 C 18.00 16.00 19.00 13.00 25.00 16.00 11.00 28.00 26.00 11.00 12.00 23.00 D 18.00 20.00 32.00 18.00 16.00 17.00 20.00 24.00 23.00 19.00 16.00 11.00 E 23.00 11.00 23.00 19.00 21.00 23.00 7.00 12.00 19.00 13.00 19.00 27.00 F 21.00 13.00 12.00 19.00 27.00 16.00 16.00 11.00 21.00 23.00 24.00 22.00 G 17.00 18.00 8.00 12.00 18.00 32.00 22.00 17.00 19.00 19.00 33.00 17.00 H 12.00 12.00 10.00 28.00 16.00 25.00 11.00 16.00 19.00 12.00 24.00 16.00