Load time: 06/07/2012 02:11:51 PM Read time: 06/07/2012 02:11:51 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2641 Comment: Block-8_plate_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 92372048 cps Min counts: 17032 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 87225864.00 66558848.00 92372048.00 34064.00 3067822.00 4744125.00 8557575.00 5880692.00 2696803.00 3563144.00 4716542.00 7625639.00 B 63003244.00 61799096.00 77249784.00 35084.00 2352783.00 2847531.00 4394370.00 5554289.00 3422992.00 10238630.00 7991399.00 7338116.00 C 30829712.00 37805516.00 48817216.00 34914.00 6307039.00 1585574.00 3629338.00 4646267.00 9289325.00 9464634.00 5653742.00 5277291.00 D 17114012.00 20206664.00 17371822.00 35990.00 6204275.00 3042026.00 7091424.00 10081550.00 2649824.00 3794484.00 7438987.00 6674655.00 E 11489781.00 8119956.00 10403829.00 36255.00 5865876.00 3749452.00 3075608.00 3341449.00 4678484.00 354789.00 4207500.00 4914302.00 F 5122387.00 5650586.00 7324419.00 35310.00 7531167.00 1473445.00 5573232.00 11972057.00 810545.00 6342067.00 4989133.00 4214854.00 G 3724594.00 2886188.00 3687091.00 35122.00 3212210.00 2167428.00 20516804.00 5170215.00 5614376.00 1636538.00 4530007.00 8850790.00 H 2890668.00 2070983.00 2971263.00 35461.00 10901664.00 853484.00 4877947.00 7074061.00 6809215.00 16185670.00 6368138.00 16510030.00 Load time: 06/07/2012 02:13:34 PM Read time: 06/07/2012 02:13:34 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2642 Comment: Block-8_plate_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 16312044 cps Min counts: 1029 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3843577.00 2661492.00 3025121.00 3602225.00 1683943.00 791907.00 2209.00 2284.00 2228.00 2473.00 2341.00 2247.00 B 3532241.00 10127582.00 5312580.00 1765453.00 936304.00 2398.00 2303.00 2209.00 2360.00 2247.00 2360.00 2341.00 C 13201368.00 3463352.00 12200914.00 7295884.00 573282.00 2247.00 2228.00 2209.00 2435.00 2473.00 2322.00 2360.00 D 10125395.00 3605722.00 7844231.00 11743384.00 1199153.00 2379.00 2209.00 2190.00 2454.00 2284.00 2284.00 2095.00 E 9966347.00 6701431.00 7186124.00 8055764.00 947275.00 2265.00 2190.00 2738.00 2492.00 2228.00 2247.00 2152.00 F 15819463.00 6011506.00 11874841.00 6264004.00 624171.00 2265.00 2322.00 2322.00 2303.00 2360.00 2247.00 2058.00 G 6136020.00 6110506.00 6588219.00 7385802.00 643111.00 2322.00 2360.00 2190.00 2171.00 2190.00 2341.00 2228.00 H 4557220.00 7343194.00 8474667.00 16312044.00 195323.00 2530.00 2303.00 2265.00 2322.00 2209.00 2265.00 2228.00 Load time: 06/07/2012 02:15:41 PM Read time: 06/07/2012 02:15:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2643 Comment: Block-12_plate_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.3 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 101508024 cps Min counts: 16749 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 101508024.00 84707680.00 82757192.00 38483.00 904581.00 919990.00 3450700.00 2566902.00 2484997.00 3366788.00 2733246.00 2210647.00 B 77264456.00 52799252.00 63946060.00 38067.00 3223576.00 1771526.00 4838805.00 679743.00 1488363.00 3537472.00 3342122.00 2818916.00 C 63430552.00 33674256.00 43404888.00 37539.00 2624137.00 3774050.00 2923799.00 1086234.00 1206763.00 3191440.00 2547190.00 2339748.00 D 26727406.00 18825784.00 21578622.00 37331.00 4011044.00 1997195.00 5687839.00 1019483.00 1753269.00 1136953.00 2718675.00 5552699.00 E 13558972.00 9334461.00 11909379.00 37633.00 4132559.00 3034727.00 2458083.00 2849988.00 1644801.00 969217.00 1693955.00 1908761.00 F 8184588.00 9447627.00 8270577.00 33498.00 2134428.00 1214128.00 2377738.00 4593809.00 2647349.00 986816.00 2735592.00 3295771.00 G 4742700.00 2755215.00 3788052.00 39540.00 3768493.00 2616226.00 3430417.00 1167544.00 1913695.00 4431158.00 470616.00 5231969.00 H 2908422.00 2059626.00 2738479.00 36424.00 3113689.00 1581654.00 2977325.00 2270142.00 3327760.00 7345308.00 1664844.00 4005821.00 Load time: 06/07/2012 02:17:37 PM Read time: 06/07/2012 02:17:37 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2644 Comment: Block-12_plate_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 5085596 cps Min counts: 1066 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3012517.00 535800.00 773100.00 1762568.00 854919.00 378241.00 223950.00 2247.00 2341.00 2303.00 2360.00 2228.00 B 1076226.00 1059213.00 2294580.00 1713469.00 935360.00 523016.00 184881.00 2171.00 2209.00 2209.00 2284.00 2209.00 C 2599106.00 1659487.00 2659807.00 3126832.00 558629.00 424391.00 135767.00 2303.00 2398.00 2398.00 2190.00 2303.00 D 1343078.00 2554035.00 2555956.00 3597372.00 473713.00 1024487.00 240435.00 2152.00 2341.00 2417.00 2303.00 2417.00 E 2069740.00 1824638.00 1800220.00 4981258.00 359000.00 550793.00 2228.00 2247.00 2379.00 2492.00 2322.00 2322.00 F 2630002.00 3727335.00 3868702.00 5085596.00 568486.00 788490.00 2303.00 2303.00 2360.00 2265.00 2379.00 2209.00 G 2333480.00 4157651.00 1214411.00 2339418.00 958416.00 198024.00 2322.00 2171.00 2190.00 2379.00 2586.00 2284.00 H 1512835.00 1722877.00 1303349.00 4376933.00 688448.00 204746.00 2265.00 2133.00 2209.00 2322.00 2492.00 2284.00