Load time: 06/15/2012 02:24:01 PM Read time: 06/15/2012 02:24:01 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2670 Comment: Blk_9_p_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 33963396 cps Min counts: 5647 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 19390776.00 20458780.00 29225560.00 15167.00 4102467.00 1601715.00 7587004.00 2016302.00 2003275.00 6037918.00 9644324.00 5899241.00 B 15073814.00 16911614.00 33963396.00 18567.00 5865194.00 5104126.00 4258818.00 3860514.00 4430712.00 2584834.00 7057818.00 5476693.00 C 13618167.00 21835960.00 18158364.00 12050.00 6759191.00 2746184.00 5481306.00 2152546.00 5284004.00 6642954.00 5553339.00 2620911.00 D 14638437.00 16359885.00 17423966.00 13826.00 8346637.00 3437839.00 6718367.00 2395401.00 3928313.00 4513587.00 8187144.00 1660622.00 E 13868908.00 14788785.00 14202719.00 16243.00 4855251.00 4711580.00 2315433.00 2204889.00 4060671.00 3044538.00 7486482.00 615244.00 F 5575362.00 5018617.00 3443766.00 11295.00 3914660.00 1686065.00 2086181.00 4315295.00 2179961.00 5607626.00 6572858.00 1965540.00 G 3440801.00 3546863.00 3429756.00 12333.00 2885048.00 3782023.00 5107651.00 2657297.00 3651656.00 5478135.00 4109803.00 6880712.00 H 2660638.00 2315840.00 2785077.00 12598.00 1726614.00 2810936.00 2734618.00 1552993.00 3168105.00 4601161.00 1952179.00 1093265.00 Load time: 06/15/2012 02:26:01 PM Read time: 06/15/2012 02:26:01 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2671 Comment: Blk_9_p_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 9116682 cps Min counts: 1010 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3772919.00 5298152.00 2493198.00 5505565.00 995349.00 417163.00 434371.00 2247.00 2266.00 2228.00 2323.00 2304.00 B 2101526.00 5526029.00 9116682.00 2738951.00 694007.00 692496.00 304420.00 2209.00 2342.00 2247.00 2058.00 2342.00 C 1416914.00 2738710.00 3831594.00 5932335.00 472430.00 286741.00 723435.00 2379.00 2398.00 2209.00 2247.00 2134.00 D 4526171.00 3121186.00 3742791.00 4438762.00 507940.00 360027.00 2209.00 2266.00 2398.00 2172.00 2398.00 2228.00 E 1679948.00 4040322.00 3187326.00 3398883.00 946599.00 235648.00 2304.00 2455.00 2304.00 2285.00 2191.00 2247.00 F 3972978.00 2340024.00 4209780.00 4745608.00 636115.00 440339.00 2209.00 2228.00 2247.00 2172.00 2021.00 2247.00 G 3450827.00 2256162.00 3651732.00 3886911.00 1826543.00 109003.00 2077.00 2096.00 2379.00 2266.00 2587.00 2228.00 H 1661229.00 2219450.00 3356375.00 1484949.00 187899.00 88963.00 2077.00 2172.00 2266.00 2266.00 2115.00 2228.00 Load time: 06/15/2012 02:28:11 PM Read time: 06/15/2012 02:28:11 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2672 Comment: Blk_10_p_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31554606 cps Min counts: 4731 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27197556.00 28206084.00 31554606.00 12503.00 7838855.00 4939730.00 3429371.00 1662877.00 2729325.00 2141407.00 3557890.00 3589723.00 B 25883278.00 26703128.00 15093312.00 11880.00 6336465.00 6351014.00 6161835.00 2268222.00 1548793.00 1134781.00 5555305.00 3025279.00 C 18524942.00 16397403.00 12996403.00 10879.00 6859445.00 8183889.00 4609651.00 1351561.00 1919541.00 2613630.00 4252035.00 2240029.00 D 24595348.00 13912578.00 19055170.00 11351.00 10875207.00 6344218.00 4284946.00 3962290.00 3090625.00 3418908.00 4836124.00 2162764.00 E 12779493.00 10633655.00 12072067.00 9462.00 3916263.00 8028465.00 1875139.00 677046.00 2197654.00 1222479.00 2412390.00 2330704.00 F 6320181.00 5544897.00 6608708.00 24327.00 4113767.00 5286044.00 2159024.00 3797136.00 1656343.00 1011782.00 1056547.00 3664211.00 G 4985491.00 4018339.00 4153042.00 25895.00 6656459.00 10625126.00 3758105.00 3969990.00 4482594.00 2189635.00 1468403.00 1355792.00 H 3506022.00 3158149.00 3098999.00 27425.00 3841882.00 4283143.00 2225236.00 2713501.00 2772895.00 2025359.00 1906531.00 3179023.00 Load time: 06/15/2012 02:30:01 PM Read time: 06/15/2012 02:30:01 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2673 Comment: Blk_10_p_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 5773476 cps Min counts: 1038 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1565761.00 1182304.00 953191.00 5773476.00 754468.00 363729.00 2266.00 2134.00 2285.00 2361.00 2361.00 2379.00 B 2454374.00 2199958.00 1748222.00 1404127.00 658063.00 382239.00 2304.00 2436.00 2323.00 2323.00 2191.00 2077.00 C 2329969.00 1573460.00 1666564.00 2813267.00 130196.00 340232.00 2285.00 2304.00 2266.00 2417.00 2342.00 2342.00 D 1629106.00 670057.00 2070544.00 2613219.00 40269.00 469427.00 2096.00 2417.00 2493.00 2209.00 2587.00 2436.00 E 1261502.00 2111351.00 2126607.00 1502288.00 257917.00 122716.00 2096.00 2247.00 2436.00 2304.00 2417.00 2247.00 F 1576594.00 1963729.00 1783593.00 1215377.00 478814.00 507279.00 2379.00 2323.00 2096.00 2361.00 2247.00 2342.00 G 1446682.00 1358247.00 2023383.00 2597914.00 246981.00 226355.00 2153.00 2436.00 2228.00 2228.00 2342.00 2417.00 H 1246014.00 1985391.00 1058549.00 3348947.00 278902.00 90870.00 2304.00 2209.00 2342.00 2266.00 2304.00 2342.00 Load time: 06/15/2012 02:32:34 PM Read time: 06/15/2012 02:32:34 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2674 Comment: Blk_11_p_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 65196896 cps Min counts: 10841 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 37102388.00 64059996.00 53001236.00 29862.00 16679760.00 13125278.00 9298685.00 3037961.00 7219837.00 11739626.00 15910595.00 10592323.00 B 43077260.00 65196896.00 46914868.00 29201.00 18519046.00 15107135.00 7806481.00 5730345.00 8071206.00 10547571.00 15520331.00 15251815.00 C 36008760.00 32549078.00 27824756.00 29219.00 17984206.00 10114883.00 7364498.00 4686309.00 11877266.00 10994941.00 15499032.00 7435332.00 D 24524088.00 24998756.00 20851038.00 27897.00 3736318.00 16894368.00 8042670.00 5387473.00 10486425.00 8261218.00 7014935.00 9584488.00 E 12297928.00 8534799.00 13053901.00 28067.00 13762672.00 14834809.00 10571134.00 8752492.00 10813372.00 10415049.00 8194012.00 6914596.00 F 7433303.00 7364870.00 9585094.00 27350.00 6690549.00 11437350.00 21411778.00 14721080.00 4860353.00 9519977.00 13415951.00 4710714.00 G 4880492.00 5693173.00 4346325.00 26783.00 11443695.00 15686485.00 13214301.00 8679428.00 7588932.00 5952789.00 4587884.00 3706887.00 H 2205920.00 2313129.00 2179638.00 21683.00 12805086.00 11490025.00 4218003.00 13398572.00 10833208.00 9602083.00 12666240.00 4544530.00 Load time: 06/15/2012 02:34:22 PM Read time: 06/15/2012 02:34:22 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2675 Comment: Blk_11_p_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 13890373 cps Min counts: 1029 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8983353.00 3703950.00 1856191.00 5851132.00 865814.00 969718.00 105301.00 388000.00 2172.00 2058.00 2115.00 2096.00 B 1130305.00 5791338.00 4355842.00 1792442.00 464554.00 93269.00 192885.00 34300.00 2228.00 2228.00 2285.00 2398.00 C 1035052.00 1603485.00 847776.00 3507967.00 293352.00 242259.00 23874.00 243676.00 2209.00 2342.00 2304.00 2191.00 D 3814999.00 362256.00 8465634.00 3777921.00 152238.00 97651.00 55795.00 158093.00 2436.00 2209.00 2304.00 5099.00 E 4128669.00 7386274.00 2449227.00 1932432.00 104715.00 72133.00 284531.00 88944.00 2209.00 2115.00 2531.00 2172.00 F 1035373.00 4381440.00 1537571.00 6972658.00 166083.00 66316.00 38890.00 110949.00 2304.00 2115.00 2228.00 2191.00 G 4740383.00 6922368.00 2410208.00 874408.00 216873.00 179758.00 64030.00 538954.00 2285.00 63388.00 2115.00 2323.00 H 2381361.00 1474901.00 5001975.00 13890373.00 205427.00 48334.00 99710.00 85884.00 2266.00 2115.00 2247.00 2172.00