Load time: 06/20/2012 12:01:04 PM Read time: 06/20/2012 12:01:04 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2677 Comment: standards+blank Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 26404776 cps Min counts: 1028 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 26404776.00 19714310.00 18828386.00 13251.00 2246.00 2302.00 2189.00 2321.00 2208.00 2378.00 2227.00 2151.00 B 25982070.00 10802649.00 11918801.00 16385.00 2265.00 2208.00 2246.00 2208.00 2321.00 2246.00 2397.00 2208.00 C 17693620.00 8321972.00 6150572.00 14761.00 2227.00 2133.00 2472.00 2189.00 2265.00 2208.00 2435.00 2302.00 D 9542580.00 5034521.00 14222951.00 13855.00 2189.00 2057.00 2189.00 2510.00 2095.00 2359.00 2359.00 2302.00 E 5562715.00 3423420.00 3220408.00 15082.00 2208.00 2265.00 2227.00 2208.00 2397.00 2265.00 10401.00 2302.00 F 4988778.00 2258118.00 6385410.00 13270.00 2416.00 2284.00 2189.00 2189.00 2340.00 2246.00 2435.00 2265.00 G 2181526.00 1823383.00 2291473.00 14893.00 2265.00 2208.00 2189.00 2378.00 2189.00 2246.00 2416.00 2378.00 H 2330780.00 1088363.00 1217065.00 97876.00 2151.00 2208.00 2114.00 2453.00 2340.00 2265.00 2265.00 2265.00 Load time: 06/20/2012 12:03:23 PM Read time: 06/20/2012 12:03:23 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2678 Comment: Blk_10_p_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10120078 cps Min counts: 321482 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 5865044.00 4556835.00 2704853.00 2202600.00 2611135.00 1785360.00 3322245.00 3446399.00 1458877.00 1410137.00 892421.00 5102443.00 B 8550547.00 5794776.00 4607936.00 2351537.00 1271015.00 849703.00 4905450.00 2784635.00 2129711.00 3009399.00 2393980.00 1784542.00 C 6472209.00 7762552.00 4658032.00 1426334.00 1770357.00 2597146.00 3698012.00 2152037.00 2087015.00 1913781.00 2351088.00 2400613.00 D 10120078.00 5726294.00 3026585.00 3552052.00 2642038.00 3183600.00 5832037.00 1914655.00 1752078.00 642964.00 2750831.00 3279310.00 E 5153010.00 9325425.00 1486173.00 680528.00 1775155.00 1464502.00 2213673.00 2042516.00 1252685.00 3057261.00 2084604.00 2138924.00 F 3665834.00 4610816.00 2007450.00 3405211.00 1402888.00 863426.00 1058122.00 2992533.00 1468485.00 2686712.00 2231959.00 1571331.00 G 5779819.00 7524678.00 2827156.00 2626708.00 3785552.00 1664592.00 1285795.00 2166699.00 1678503.00 1741748.00 2528174.00 2537199.00 H 3108094.00 3395888.00 2116836.00 2266250.00 2290298.00 1843011.00 1748411.00 3210415.00 1739700.00 2181457.00 1028183.00 4329872.00 Load time: 06/20/2012 12:05:25 PM Read time: 06/20/2012 12:05:25 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2679 Comment: Blk_10_p_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 1309373 cps Min counts: 1057 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 725210.00 349580.00 1040755.00 2397.00 2265.00 2321.00 2548.00 2265.00 2491.00 2340.00 2170.00 8268.00 B 707088.00 336366.00 1309373.00 2208.00 2284.00 2359.00 2208.00 2340.00 2397.00 2340.00 2302.00 21802.00 C 124096.00 305181.00 521208.00 2302.00 15233.00 2265.00 2302.00 2246.00 2510.00 2453.00 2340.00 22878.00 D 27239.00 580746.00 2170.00 2208.00 2321.00 2340.00 2284.00 2321.00 2416.00 2359.00 2302.00 17385.00 E 232090.00 89929.00 2189.00 2189.00 2453.00 2246.00 2208.00 2378.00 2453.00 2416.00 2227.00 7569.00 F 601246.00 639509.00 2246.00 2227.00 2359.00 2246.00 2321.00 2491.00 2359.00 2302.00 2246.00 10363.00 G 235186.00 211760.00 2246.00 2189.00 2416.00 2397.00 2359.00 2435.00 2265.00 2416.00 2340.00 11873.00 H 307598.00 66786.00 2302.00 2284.00 2189.00 2170.00 2189.00 2189.00 2114.00 2246.00 2302.00 7305.00 Load time: 06/20/2012 12:07:53 PM Read time: 06/20/2012 12:07:53 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2680 Comment: Blk_11_p_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 18330110 cps Min counts: 216809 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11033510.00 11304734.00 10809082.00 3310101.00 6656971.00 9758918.00 13215837.00 10182170.00 9646636.00 3199412.00 2115028.00 6907957.00 B 13094684.00 10024040.00 7383509.00 5387515.00 7527490.00 7428326.00 16461540.00 14036352.00 1182294.00 5390133.00 4185637.00 1795404.00 C 8799589.00 8674769.00 7450667.00 3784051.00 9126586.00 10152301.00 11687400.00 5604042.00 1068957.00 1626710.00 704427.00 2972435.00 D 2253962.00 12912023.00 7867950.00 5149461.00 8523373.00 5001842.00 5337829.00 8659439.00 3336879.00 433619.00 8635860.00 3341077.00 E 10252172.00 9851604.00 9387288.00 8725824.00 9720334.00 7944181.00 7297799.00 5289322.00 3915400.00 7171260.00 2444130.00 1829616.00 F 4771747.00 9595874.00 14010916.00 11883939.00 3484611.00 6803487.00 9432235.00 3749850.00 696536.00 3751661.00 1415762.00 6712358.00 G 10002656.00 15247994.00 13874572.00 8295214.00 7554335.00 4138092.00 5475983.00 4475872.00 5642764.00 6102206.00 2035449.00 742407.00 H 9202580.00 11441929.00 4356484.00 14489487.00 11471059.00 8483572.00 11110962.00 6224221.00 2960478.00 1996526.00 5331219.00 18330110.00 Load time: 06/20/2012 12:09:36 PM Read time: 06/20/2012 12:09:36 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2681 Comment: Blk_11_p_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 2181693 cps Min counts: 1038 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 954658.00 877697.00 81057.00 318150.00 1930124.00 2073465.00 327777.00 437659.00 2359.00 2284.00 2076.00 2246.00 B 583728.00 66993.00 141878.00 30467.00 1344238.00 675583.00 1158792.00 821822.00 2359.00 2359.00 2302.00 2284.00 C 301576.00 349221.00 26295.00 154827.00 839075.00 465597.00 612855.00 778801.00 2302.00 2302.00 2397.00 2208.00 D 161547.00 97989.00 38773.00 163530.00 1334064.00 1024144.00 855857.00 1230203.00 2284.00 2265.00 2284.00 2472.00 E 90948.00 51911.00 295970.00 75073.00 1550202.00 701161.00 1993029.00 582595.00 2321.00 2453.00 2529.00 2227.00 F 148447.00 64974.00 26521.00 84473.00 551770.00 385672.00 2181693.00 6229.00 2284.00 2472.00 2378.00 2133.00 G 198301.00 149315.00 53704.00 559754.00 957886.00 1426183.00 400226.00 13742.00 2265.00 2284.00 2378.00 2227.00 H 202586.00 35696.00 76432.00 101160.00 617499.00 128249.00 1643319.00 10722.00 2208.00 4719.00 2397.00 2284.00 Load time: 06/20/2012 12:12:00 PM Read time: 06/20/2012 12:12:00 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2682 Comment: Blk_12_p_1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 23707608 cps Min counts: 241585 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 926437.00 934988.00 2788427.00 2575995.00 2397628.00 2853814.00 2368532.00 3077884.00 2821948.00 492043.00 920283.00 2212461.00 B 3964514.00 1576882.00 4497177.00 801208.00 1777729.00 3548948.00 4430633.00 3771509.00 965795.00 852723.00 1939492.00 1284191.00 C 2674981.00 3316073.00 2371538.00 1460973.00 1373894.00 3561910.00 3153324.00 2684048.00 2456279.00 1734750.00 2577683.00 3050908.00 D 2958739.00 1742182.00 6958032.00 1356319.00 1216857.00 1045342.00 2193058.00 4305066.00 1287985.00 3048305.00 2590616.00 3903981.00 E 4409188.00 3785024.00 2713880.00 2861967.00 2154517.00 781142.00 2523515.00 2193378.00 3232685.00 1904182.00 2102162.00 5283344.00 F 1628368.00 1229033.00 1900346.00 4854915.00 4600496.00 1161133.00 3987493.00 3623956.00 3912515.00 4789965.00 4510207.00 5262140.00 G 4121512.00 2340159.00 4120877.00 1594195.00 2764336.00 4248921.00 483171.00 7016450.00 2380180.00 4775259.00 2272705.00 2714189.00 H 4825112.00 2210351.00 4170198.00 2818869.00 5364222.00 23707608.00 2007872.00 4301288.00 1746767.00 1877865.00 1679782.00 4911721.00 Load time: 06/20/2012 12:14:03 PM Read time: 06/20/2012 12:14:03 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2683 Comment: Blk_12_p_2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 3328859 cps Min counts: 1019 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 864332.00 392543.00 251496.00 3328859.00 596017.00 13062.00 2038.00 2359.00 2265.00 2472.00 2189.00 169155.00 B 833884.00 470486.00 224917.00 1916732.00 599773.00 75998.00 2170.00 2265.00 2227.00 2359.00 2340.00 385068.00 C 523417.00 677131.00 177838.00 2212377.00 490269.00 5361.00 2340.00 2265.00 2227.00 2359.00 2491.00 237942.00 D 337064.00 1036678.00 2378.00 348674.00 2246.00 2359.00 2189.00 2265.00 2321.00 2321.00 2302.00 505597.00 E 255818.00 566607.00 2189.00 450458.00 2284.00 2227.00 2227.00 2416.00 2302.00 2246.00 2793.00 413138.00 F 772704.00 625559.00 2397.00 1510675.00 2265.00 2189.00 2340.00 2359.00 2397.00 2246.00 2189.00 825144.00 G 725606.00 252062.00 2378.00 822501.00 2284.00 2321.00 2189.00 2246.00 2340.00 2265.00 2284.00 2359.00 H 521378.00 199735.00 2114.00 644776.00 2246.00 2133.00 2170.00 2359.00 2133.00 2302.00 2151.00 2227.00