Load time: 06/27/2012 10:58:34 AM Read time: 06/27/2012 10:58:34 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2710 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 37069340 cps Min counts: 1001 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 36634552.00 32545862.00 36481500.00 33187650.00 36383024.00 37069340.00 13448.00 2191.00 2266.00 2247.00 2077.00 2247.00 B 25831986.00 25469038.00 31296588.00 27227442.00 31123474.00 25314950.00 13014.00 2172.00 2304.00 2172.00 2191.00 2285.00 C 21375420.00 21384812.00 24606508.00 25193688.00 24513418.00 23470022.00 11805.00 2285.00 2304.00 2342.00 2304.00 2153.00 D 19580546.00 20502188.00 21304138.00 21376424.00 19670206.00 26008440.00 13807.00 2209.00 2323.00 2247.00 2323.00 2115.00 E 14839751.00 14690271.00 14060907.00 12321517.00 12238330.00 14406226.00 13883.00 2361.00 2247.00 2002.00 2228.00 2172.00 F 9857333.00 8674651.00 6642841.00 7680659.00 6933326.00 7704730.00 13901.00 2285.00 2398.00 2153.00 2379.00 2153.00 G 4792443.00 6291840.00 6161096.00 4629597.00 3895555.00 5701838.00 13618.00 2153.00 2379.00 2209.00 2228.00 2209.00 H 3500118.00 3163419.00 3406425.00 3117882.00 3282721.00 3363518.00 12258.00 2153.00 2077.00 2077.00 2039.00 2247.00 Load time: 06/27/2012 11:00:48 AM Read time: 06/27/2012 11:00:48 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2711 Comment: samples 1-90 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 22367140 cps Min counts: 1019 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 11966005.00 2777799.00 2063800.00 3548246.00 10021028.00 336497.00 15429343.00 374482.00 97181.00 6532928.00 384549.00 832866.00 B 22074092.00 14346439.00 322104.00 14244770.00 544667.00 3184351.00 116088.00 135146.00 348642.00 278868.00 306578.00 4099140.00 C 13218178.00 2864966.00 14781912.00 2335970.00 1753276.00 12756378.00 4617842.00 2003901.00 956454.00 239826.00 80332.00 2058.00 D 22367140.00 1211391.00 3831277.00 2929354.00 1011419.00 1956342.00 4964085.00 285801.00 5186783.00 386023.00 10730430.00 2058.00 E 7601288.00 13504270.00 6267659.00 1386713.00 8863027.00 1611652.00 9691572.00 414714.00 49034.00 340407.00 1005450.00 2077.00 F 22008910.00 270104.00 1385712.00 2404998.00 1535498.00 18080594.00 306521.00 281645.00 124153.00 80049.00 423157.00 2039.00 G 22317976.00 15392416.00 8043232.00 2724919.00 2297268.00 3585486.00 180271.00 143514.00 6056802.00 312018.00 3891843.00 2039.00 H 2548685.00 6069446.00 12935557.00 2774060.00 3728115.00 3145521.00 280040.00 221769.00 89663.00 177910.00 2534597.00 2191.00