Load time: 07/10/2012 12:11:35 PM Read time: 07/10/2012 12:11:35 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2749 Comment: samples 1-90 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 35873320 cps Min counts: 1020 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35873320.00 29925500.00 35242840.00 14891.00 29789482.00 29461122.00 2267.00 2078.00 2305.00 2305.00 2173.00 2192.00 B 30728968.00 33107566.00 26103446.00 16535.00 20141950.00 21139546.00 2229.00 2173.00 2305.00 2437.00 2192.00 2248.00 C 27841484.00 25234732.00 23365472.00 14550.00 18570942.00 18415288.00 2267.00 2267.00 2381.00 2286.00 2305.00 2229.00 D 21467578.00 19942418.00 18898816.00 15250.00 16896048.00 18101468.00 2135.00 2305.00 2399.00 2305.00 2418.00 2248.00 E 11983728.00 11200352.00 11900816.00 14588.00 10534299.00 9199152.00 2097.00 2135.00 2381.00 2173.00 4988.00 2229.00 F 6330404.00 6322446.00 6811698.00 12869.00 7414895.00 6238751.00 2116.00 2324.00 2267.00 2210.00 2324.00 2362.00 G 3848487.00 4091745.00 3651854.00 13889.00 3924611.00 3926500.00 2305.00 2210.00 2286.00 2154.00 2399.00 2437.00 H 2309755.00 2236694.00 2074638.00 15949.00 2208361.00 2510678.00 2116.00 2267.00 2135.00 2286.00 2040.00 2437.00 Load time: 07/10/2012 12:13:32 PM Read time: 07/10/2012 12:13:32 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2750 Comment: samples 1-90 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 49782936 cps Min counts: 1058 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35088032.00 8279131.00 7686797.00 13453501.00 26660894.00 1124467.00 41160036.00 1212075.00 934379.00 27024382.00 680134.00 3288714.00 B 49029276.00 38966920.00 1822720.00 36608136.00 2210063.00 8227159.00 549156.00 1383266.00 3348735.00 2397711.00 3868142.00 19564570.00 C 32995508.00 12462647.00 31816632.00 7006129.00 5114030.00 37095696.00 8933716.00 10314554.00 7329946.00 1356035.00 2029469.00 2210.00 D 49782936.00 4530693.00 9468202.00 11307375.00 3864037.00 5013729.00 15211009.00 1199943.00 21122472.00 3839436.00 18601246.00 2324.00 E 21090988.00 27386868.00 19533810.00 5800535.00 24819156.00 3492909.00 22355448.00 3462080.00 222270.00 1926252.00 5108592.00 2248.00 F 44458452.00 1273548.00 4033228.00 6947945.00 2129865.00 47444312.00 1965794.00 2250080.00 709198.00 1580615.00 2348081.00 2116.00 G 35125776.00 28976078.00 22439962.00 7497579.00 7578214.00 9643265.00 958378.00 1122256.00 29383396.00 2005889.00 15333498.00 2267.00 H 10919095.00 16769723.00 36128332.00 7905262.00 6195090.00 13031222.00 974195.00 1490074.00 596230.00 1283772.00 15601361.00 2192.00