Load time: 07/16/2013 02:07:51 PM Read time: 07/16/2013 02:07:51 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3134 Comment: standard Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.1 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 69000720 cps Min counts: 9416 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 38979548.00 48478968.00 53583276.00 22440.00 60763288.00 65601024.00 69000720.00 65911756.00 24008.00 44920696.00 52601864.00 23366.00 B 35800948.00 39588868.00 44719216.00 19361.00 48715228.00 64537264.00 53203028.00 51943944.00 23932.00 60293092.00 58137036.00 22723.00 C 37484800.00 27337082.00 35659208.00 21439.00 46852596.00 39844940.00 43087024.00 42300692.00 24461.00 41119748.00 43328316.00 22553.00 D 24816614.00 20543224.00 23297634.00 20343.00 33918220.00 34329368.00 30591626.00 22465826.00 24650.00 26282168.00 26515818.00 23592.00 E 12882866.00 13891373.00 14689868.00 22100.00 16151630.00 18093242.00 18801928.00 11452101.00 22931.00 14972057.00 12921271.00 24310.00 F 8273619.00 7783988.00 10817540.00 18832.00 6929580.00 7589503.00 8803326.00 5934363.00 24329.00 9437476.00 10171403.00 22497.00 G 4823305.00 3141235.00 4494827.00 19701.00 4353314.00 5005068.00 4423001.00 4854430.00 24782.00 5321331.00 7277697.00 24707.00 H 2631563.00 2308692.00 2863382.00 20268.00 3924803.00 3240532.00 2206186.00 3159701.00 23536.00 3406961.00 2538612.00 28957.00 Load time: 07/16/2013 02:10:04 PM Read time: 07/16/2013 02:10:04 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3135 Comment: plate1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.2 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 23534610 cps Min counts: 11645 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35058.00 4773149.00 16810392.00 2875859.00 4457727.00 1382544.00 2356800.00 92557.00 10506373.00 5096277.00 963051.00 5682143.00 B 9072399.00 4945916.00 9207344.00 8052323.00 4619200.00 640101.00 4325562.00 1168566.00 23534610.00 9028675.00 7738822.00 6630090.00 C 5500784.00 5979921.00 15226248.00 904097.00 1338683.00 3104147.00 2740501.00 3597127.00 7582749.00 3526966.00 12645835.00 24291.00 D 7801129.00 2166795.00 43558.00 2482567.00 3554282.00 1219001.00 5133659.00 4170502.00 5592245.00 6434892.00 8731331.00 25746.00 E 4977474.00 4583550.00 9260860.00 2185570.00 3436601.00 2360675.00 2560751.00 4766306.00 4235358.00 6092607.00 6323640.00 24858.00 F 8018999.00 8001376.00 9833417.00 4261473.00 3745660.00 3535406.00 8731557.00 7935013.00 8645367.00 5335919.00 8504309.00 25557.00 G 12760019.00 8027284.00 8040591.00 4744639.00 3725357.00 1344293.00 39346.00 8129843.00 7251701.00 7113600.00 6195736.00 23517.00 H 13474300.00 7133098.00 5388530.00 789364.00 3052063.00 3740687.00 47771.00 1390364.00 4139034.00 4704724.00 19110904.00 23290.00 Load time: 07/16/2013 02:12:01 PM Read time: 07/16/2013 02:12:01 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3136 Comment: plate2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.3 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 60658484 cps Min counts: 11229 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 15181195.00 15708948.00 7082221.00 12140454.00 11197619.00 27312668.00 37038516.00 29167798.00 6658580.00 36653024.00 13452908.00 607082.00 B 40726032.00 38031304.00 25042728.00 25934798.00 24547648.00 25491242.00 35495780.00 6083246.00 14363888.00 39162644.00 38948936.00 13398581.00 C 3514092.00 14700865.00 25950022.00 8085291.00 21076540.00 5952181.00 18166866.00 16887150.00 23742022.00 28226594.00 24448052.00 23932.00 D 17411828.00 18102006.00 27021824.00 30828218.00 26544294.00 5855870.00 22272904.00 35156904.00 42357664.00 50331116.00 2358175.00 23366.00 E 11211085.00 33870108.00 5642239.00 14078550.00 28797064.00 14697179.00 458934.00 16844172.00 3600874.00 31483934.00 32777310.00 22912.00 F 8056616.00 4438504.00 4606815.00 60658484.00 24291236.00 54516220.00 27275406.00 57683220.00 305439.00 3971987.00 42458540.00 23498.00 G 6361623.00 22461588.00 1120512.00 23099948.00 33156704.00 33898600.00 32082908.00 26745612.00 8483580.00 14142138.00 55291500.00 22572.00 H 10507186.00 14421988.00 28315952.00 54125308.00 15872110.00 46865368.00 29711948.00 21914452.00 4902487.00 8915409.00 14638394.00 22459.00 Load time: 07/16/2013 02:14:09 PM Read time: 07/16/2013 02:14:09 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3137 Comment: plate3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.4 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 83628416 cps Min counts: 10908 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 923024.00 1080505.00 37531896.00 14029001.00 52165988.00 48548028.00 27136786.00 49069296.00 47429904.00 53750048.00 25244386.00 23288936.00 B 46135156.00 505987.00 42582632.00 718926.00 33968548.00 43556624.00 51466768.00 56919792.00 56559456.00 51600980.00 27822008.00 22909524.00 C 33388498.00 4892090.00 58946264.00 38982884.00 14867592.00 42735272.00 46830760.00 43409268.00 44758280.00 58154944.00 50980468.00 22931.00 D 13979144.00 3205307.00 21820816.00 23663672.00 45639852.00 61824684.00 60697988.00 39914208.00 31389030.00 36233852.00 59358244.00 21817.00 E 15480200.00 1325159.00 20966194.00 7688000.00 11607713.00 15859617.00 9294089.00 51280176.00 51016100.00 53809108.00 46349628.00 23177.00 F 37115632.00 32552794.00 13722409.00 27581002.00 15163938.00 43474148.00 31415796.00 67434680.00 45237444.00 79771632.00 60766152.00 24083.00 G 34318712.00 30058004.00 42641068.00 56677536.00 61973252.00 60946132.00 30183240.00 33127456.00 42617108.00 66222588.00 77150624.00 23743.00 H 51863512.00 2756673.00 41085680.00 17676888.00 57590048.00 57560484.00 62577240.00 63664232.00 26649068.00 83628416.00 77314056.00 23082.00