Load time: 07/17/2013 02:09:04 PM Read time: 07/17/2013 02:09:04 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3139 Comment: plate3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 64200528 cps Min counts: 10521 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 64200528.00 49488156.00 56432128.00 22270.00 23177.00 24631.00 24745.00 25066.00 24235.00 31564.00 24480.00 24537.00 B 45696908.00 40002308.00 30712756.00 22875.00 24499.00 25746.00 25500.00 26747.00 25311.00 26785.00 26690.00 23687.00 C 22643838.00 29860738.00 28594494.00 24291.00 24140.00 25349.00 25784.00 25935.00 26237.00 43955.00 25576.00 23668.00 D 14023063.00 13006679.00 12233114.00 31488.00 23914.00 24480.00 24801.00 25840.00 69267.00 26785.00 114149.00 22478.00 E 9819224.00 13261721.00 6392449.00 24310.00 23592.00 25141.00 25689.00 25330.00 26256.00 25538.00 25803.00 24178.00 F 4916135.00 6302377.00 2299610.00 24235.00 22894.00 24782.00 25463.00 24367.00 24291.00 25614.00 25727.00 22006.00 G 2044908.00 3582495.00 1022959.00 23592.00 24971.00 23555.00 24669.00 73687.00 24424.00 24424.00 23800.00 21042.00 H 2016217.00 2363350.00 1879625.00 23215.00 26105.00 24631.00 24650.00 25009.00 24386.00 24424.00 23800.00 21987.00 Load time: 07/17/2013 02:11:14 PM Read time: 07/17/2013 02:11:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3140 Comment: plate-1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 79184592 cps Min counts: 11909 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10655482.00 17987252.00 2935417.00 31536984.00 55369204.00 4148662.00 2271011.00 44237684.00 20862718.00 15389763.00 9125606.00 77295.00 B 25367462.00 8559734.00 16240547.00 12068687.00 9629603.00 22917080.00 54687076.00 25248504.00 853350.00 41199640.00 36085152.00 196167.00 C 911889.00 22901002.00 24038198.00 8428315.00 35757124.00 40861292.00 10372053.00 15742698.00 2132037.00 20865586.00 4304260.00 24858.00 D 4335732.00 10633237.00 12666787.00 50780212.00 11004429.00 2101283.00 12384356.00 25452080.00 25484020.00 22553932.00 50638628.00 24254.00 E 12724700.00 28798846.00 597190.00 28597136.00 3603584.00 3402113.00 10298634.00 6121958.00 8915804.00 25361892.00 31211556.00 23819.00 F 4414334.00 30795880.00 11637683.00 6298540.00 15168249.00 2406891.00 20635584.00 79184592.00 4257072.00 25225126.00 27139720.00 26577.00 G 52153.00 645944.00 405046.00 2297179.00 35766712.00 6834367.00 1598393.00 49993388.00 9415197.00 36548292.00 57325212.00 406841.00 H 5568541.00 335571.00 18774556.00 5149546.00 11751746.00 6819813.00 440445.00 19200300.00 12707750.00 916498.00 23138724.00 24782.00 Load time: 07/17/2013 02:12:57 PM Read time: 07/17/2013 02:12:57 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3141 Comment: plate-2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 20686176 cps Min counts: 11579 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 10319499.00 2084882.00 526128.00 5045535.00 676073.00 2711776.00 4427927.00 544432.00 5358777.00 5201867.00 15621187.00 52720.00 B 1749988.00 5217026.00 1941888.00 1351315.00 894605.00 1453374.00 10741100.00 1900972.00 3450662.00 2738646.00 8641542.00 872221.00 C 2045728.00 2714692.00 4129846.00 200247.00 595925.00 2892251.00 8056989.00 2249372.00 6186968.00 1968609.00 492864.00 24461.00 D 1023223.00 5909605.00 709261.00 4749439.00 307671.00 3096666.00 14397439.00 2108836.00 4048869.00 300304.00 8848802.00 27389.00 E 531719.00 1375758.00 7733198.00 2634567.00 382870.00 3058550.00 8431816.00 2466679.00 3308005.00 5907800.00 1425097.00 23234.00 F 1593310.00 6451261.00 842224.00 15311098.00 518402.00 593696.00 17200456.00 20686176.00 2050546.00 2915230.00 10376310.00 23158.00 G 1409381.00 407653.00 11922917.00 621671.00 527224.00 9800530.00 7296904.00 9078470.00 2473885.00 4498999.00 1029646.00 24254.00 H 1974000.00 3931051.00 4047696.00 3810352.00 715344.00 4017389.00 3408082.00 12647178.00 239594.00 1461516.00 1481123.00 23649.00 Load time: 07/17/2013 02:14:59 PM Read time: 07/17/2013 02:14:59 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3142 Comment: plate-3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.4 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 21754828 cps Min counts: 10700 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4754190.00 11713478.00 8483450.00 6255671.00 9668979.00 9399070.00 21754828.00 9863725.00 7987906.00 6475466.00 5510833.00 8606236.00 B 9036144.00 5685276.00 9471918.00 7775967.00 9568345.00 8550926.00 9680229.00 14065253.00 10111604.00 6885861.00 13259379.00 9460162.00 C 5621053.00 4003476.00 12399400.00 5863072.00 12839190.00 5955163.00 12104599.00 13811907.00 11614168.00 6224389.00 8568598.00 23215.00 D 8068567.00 9387119.00 7681238.00 2180006.00 4525720.00 9233311.00 11315278.00 9453354.00 9231636.00 18116680.00 3428725.00 23404.00 E 15785189.00 6758697.00 17318810.00 8486370.00 8451913.00 2021357.00 11150398.00 15447159.00 10104698.00 11122506.00 11389953.00 21401.00 F 15215378.00 10648149.00 6907341.00 10311054.00 6348344.00 10151605.00 14351746.00 12239288.00 14306922.00 15812773.00 5980064.00 22497.00 G 7419512.00 8413588.00 7851037.00 6754520.00 10777999.00 6446128.00 8098947.00 11625022.00 10943726.00 12432854.00 17519198.00 23498.00 H 10347031.00 8888651.00 9584406.00 9123066.00 6327437.00 5792858.00 13391500.00 6404117.00 9235041.00 10719315.00 11172020.00 26388.00