Load time: 07/30/2012 11:42:24 AM Read time: 07/30/2012 11:42:24 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2780 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 30222658 cps Min counts: 5812 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 27984294.00 16236286.00 22471222.00 11624.00 23013474.00 12550.00 24093854.00 12267.00 25087584.00 16802550.00 30222658.00 13155.00 B 21394342.00 7796113.00 19203442.00 12399.00 14046983.00 12399.00 16539297.00 13023.00 13423233.00 12683697.00 23085736.00 13023.00 C 15131502.00 9732721.00 16747993.00 12399.00 10336994.00 12777.00 13604719.00 13231.00 10706240.00 10286194.00 16569279.00 12834.00 D 8205804.00 8983572.00 6782883.00 22266.00 6527736.00 13098.00 6471102.00 21434.00 16952256.00 7917854.00 20856610.00 35856.00 E 7835969.00 5549562.00 8486729.00 11643.00 11212002.00 12815.00 6213376.00 12777.00 8912063.00 10459031.00 19051094.00 12645.00 F 4188088.00 4433506.00 5029296.00 11737.00 9223007.00 14043.00 5562069.00 13628.00 5596551.00 6609399.00 8690635.00 12682.00 G 3517110.00 3716594.00 2599984.00 12342.00 3523646.00 13514.00 2317794.00 17918.00 2216531.00 4383074.00 5102385.00 12909.00 H 1958456.00 1780200.00 2803983.00 11813.00 3607895.00 13533.00 2269312.00 13098.00 2144223.00 1727631.00 2436150.00 11964.00 Load time: 07/30/2012 11:44:17 AM Read time: 07/30/2012 11:44:17 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2781 Comment: p-1 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 31647338 cps Min counts: 8741 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14457096.00 4190686.00 3009047.00 16534281.00 3784634.00 19563498.00 10131078.00 31647338.00 8357644.00 6658581.00 2496832.00 3295711.00 B 11362984.00 11271583.00 9858627.00 3679154.00 9287389.00 2025859.00 11407336.00 4534802.00 8497152.00 7434276.00 7810459.00 2600251.00 C 3786570.00 10993523.00 7624688.00 7380627.00 11040287.00 9333675.00 9298230.00 10646729.00 7797377.00 6093265.00 5637691.00 4264853.00 D 7277093.00 12501418.00 4938375.00 1140085.00 10805594.00 6426225.00 18729534.00 7247040.00 11168028.00 9462341.00 6989555.00 3734840.00 E 3689564.00 4648308.00 11503574.00 1144735.00 7455360.00 8337069.00 5476732.00 14997896.00 11292348.00 16495600.00 3670797.00 17483.00 F 9005171.00 9638345.00 10231263.00 15419149.00 11504394.00 4997417.00 16504531.00 11910945.00 5659885.00 5836431.00 3591710.00 18315.00 G 6325263.00 12891926.00 10672797.00 4070889.00 6164467.00 3496537.00 3736383.00 4182786.00 13558189.00 6594963.00 6282722.00 18183.00 H 5880071.00 10123883.00 6620556.00 1007661.00 5304962.00 4826422.00 7347182.00 4135622.00 308189.00 11175475.00 4848176.00 19355.00 Load time: 07/30/2012 11:46:07 AM Read time: 07/30/2012 11:46:07 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2782 Comment: p-2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 3868178 cps Min counts: 1077 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 287341.00 3868178.00 2029047.00 461103.00 953980.00 762715.00 488510.00 2268.00 2495.00 2211.00 2324.00 2400.00 B 427099.00 424207.00 1495831.00 732511.00 376235.00 238367.00 625433.00 2230.00 2438.00 2702.00 2400.00 2343.00 C 234625.00 948253.00 1988113.00 522514.00 647718.00 488529.00 519792.00 2192.00 2343.00 3080.00 2362.00 2173.00 D 146052.00 368051.00 588462.00 235229.00 88081.00 1201912.00 731074.00 2211.00 2230.00 2230.00 2343.00 2173.00 E 173573.00 2246775.00 696295.00 246646.00 1014333.00 822860.00 2324.00 2362.00 2287.00 2419.00 2268.00 2230.00 F 652425.00 1249846.00 746933.00 852157.00 372852.00 330852.00 2324.00 2154.00 2324.00 2192.00 2192.00 5349.00 G 595777.00 1768957.00 1461204.00 543590.00 386499.00 249198.00 2324.00 2287.00 2495.00 2381.00 2457.00 2438.00 H 1124246.00 2008011.00 152403.00 1432624.00 354423.00 374534.00 2192.00 2305.00 2268.00 2495.00 2362.00 2268.00