Load time: 08/05/2013 01:47:24 PM Read time: 08/05/2013 01:47:24 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3162 Comment: std Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 26.5 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 27602916 cps Min counts: 10052 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 14911826.00 10626464.00 10608796.00 21276.00 20355294.00 17852710.00 9710927.00 24696.00 27602916.00 11200848.00 9645108.00 20861.00 B 7976542.00 5012304.00 8518371.00 20105.00 18098046.00 9585644.00 14884182.00 21843.00 22521476.00 4546889.00 5960385.00 24489.00 C 8047745.00 5024268.00 4389715.00 21106.00 9118803.00 8434316.00 21748516.00 24016.00 14707774.00 7916381.00 8553466.00 25188.00 D 6686688.00 4168547.00 5167018.00 20577.00 10750568.00 9052357.00 11801783.00 24073.00 6134864.00 4616924.00 5529050.00 25490.00 E 4567694.00 1308354.00 4384799.00 20861.00 3544894.00 5154715.00 5000261.00 24696.00 5669720.00 2840799.00 4237540.00 22523.00 F 2739344.00 2310311.00 2484588.00 28816.00 1265083.00 3955423.00 1530646.00 23789.00 3601180.00 1719206.00 1934342.00 24319.00 G 2044289.00 1159775.00 1969939.00 23563.00 683711.00 3033265.00 543692.00 25377.00 3837717.00 3803437.00 3934995.00 24413.00 H 1320221.00 459190.00 574247.00 22637.00 230284.00 2482651.00 346494.00 24678.00 4677570.00 989315.00 3665906.00 23978.00 Load time: 08/05/2013 01:49:46 PM Read time: 08/05/2013 01:49:46 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3163 Comment: Plate2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 26.6 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 11237813 cps Min counts: 11535 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 4087553.00 886446.00 430562.00 1294163.00 466351.00 1155996.00 3298354.00 194779.00 2134373.00 1975188.00 3090854.00 35051.00 B 1017130.00 1991518.00 1323887.00 1167655.00 1004243.00 1486108.00 6437773.00 4355090.00 2711066.00 3040067.00 7070017.00 681538.00 C 1385487.00 1862418.00 2863235.00 448986.00 1452020.00 3266430.00 5626353.00 2314639.00 3621906.00 1582961.00 244872.00 24772.00 D 816984.00 2715293.00 455014.00 5013869.00 603063.00 5096564.00 5487268.00 2036906.00 6260176.00 513119.00 7127809.00 31329.00 E 653591.00 779173.00 4441662.00 2694921.00 987463.00 4233989.00 8666665.00 3638793.00 1570674.00 6484998.00 564251.00 23071.00 F 1271450.00 2398905.00 789377.00 9023061.00 515726.00 515764.00 8530831.00 11237813.00 3129980.00 4133161.00 4845047.00 24659.00 G 1260850.00 261444.00 4699031.00 530786.00 440804.00 3953918.00 4559467.00 5357721.00 3107514.00 2235164.00 590573.00 1301948.00 H 1789321.00 4830967.00 4984084.00 3215275.00 651399.00 2724940.00 3616862.00 8503311.00 266564.00 1578961.00 1841072.00 438763.00 Load time: 08/05/2013 01:52:18 PM Read time: 08/05/2013 01:52:18 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #3165 Comment: Plate3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 26.7 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 18965620 cps Min counts: 80487 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 1102766.00 4163759.00 4676216.00 2571413.00 8287655.00 4289837.00 10828843.00 5126218.00 8359407.00 4258735.00 3433015.00 7017581.00 B 9554676.00 2882312.00 13833873.00 5755344.00 10299403.00 5441012.00 9262202.00 13692391.00 8867518.00 4382124.00 8943321.00 4548664.00 C 3582176.00 2598087.00 11490164.00 3704157.00 10245589.00 4814589.00 14734596.00 17927226.00 10101744.00 3971756.00 8226574.00 6540966.00 D 5788331.00 8556936.00 8959400.00 426897.00 3438107.00 4333335.00 13247549.00 12096515.00 9877390.00 11960412.00 1407407.00 5403746.00 E 10181798.00 2658070.00 12826641.00 4713965.00 6078339.00 160974.00 12356296.00 14017979.00 9158076.00 8738339.00 11338020.00 7757454.00 F 9706056.00 8260843.00 3688322.00 8116774.00 5536917.00 8747029.00 11541170.00 9896435.00 13065160.00 18965620.00 3895527.00 8192655.00 G 8510597.00 8414775.00 7918641.00 4895638.00 11532487.00 4700295.00 8212415.00 11078774.00 8081120.00 7908333.00 10854312.00 7690173.00 H 7556100.00 4225293.00 6660924.00 5865434.00 5716335.00 2857838.00 10430034.00 2447946.00 4960656.00 5837850.00 5643786.00 5351601.00