Load time: 08/27/2012 11:18:35 AM Read time: 08/27/2012 11:18:35 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2825 Comment: standards Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.4 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 64187160 cps Min counts: 1048 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 55946432.00 43397432.00 2218449.00 20765.00 52254720.00 45204512.00 2287127.00 15871.00 17609.00 2135.00 2267.00 2286.00 B 49127100.00 53231612.00 4063195.00 25923.00 48196420.00 45505116.00 4730068.00 15134.00 15455.00 2116.00 2286.00 2456.00 C 34718800.00 35757624.00 7138872.00 23996.00 35041372.00 33497680.00 7595895.00 15229.00 14737.00 2172.00 2286.00 3004.00 D 21864106.00 21231776.00 12494167.00 20727.00 21004056.00 20728618.00 14370881.00 13944.00 16060.00 2361.00 2399.00 2380.00 E 12893765.00 12478012.00 20739008.00 21407.00 12428956.00 13893978.00 22723300.00 13774.00 16173.00 2248.00 2305.00 2153.00 F 7847111.00 7413055.00 37280636.00 27718.00 7757335.00 8165932.00 33945660.00 16287.00 22220.00 2248.00 2248.00 2097.00 G 4333180.00 3947702.00 52938500.00 24827.00 4316923.00 4736914.00 52629260.00 16362.00 14926.00 2248.00 2475.00 2248.00 H 2568417.00 2324009.00 64187160.00 26981.00 2594526.00 2907517.00 51309976.00 17609.00 13282.00 2248.00 2248.00 2267.00 Load time: 08/27/2012 11:20:35 AM Read time: 08/27/2012 11:20:35 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2826 Comment: samples Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 40559916 cps Min counts: 1058 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 672610.00 888085.00 414189.00 3172187.00 976379.00 620934.00 3153182.00 3265436.00 3845700.00 2267.00 2286.00 2361.00 B 376324.00 659063.00 1272288.00 4835593.00 386319.00 1099308.00 1855626.00 1653819.00 1960684.00 2172.00 2380.00 2153.00 C 538704.00 706262.00 643456.00 2655432.00 354595.00 195219.00 832194.00 730655.00 1723561.00 2361.00 2229.00 2210.00 D 371865.00 951722.00 740140.00 8552701.00 1471475.00 1354480.00 1142217.00 882643.00 40559916.00 2324.00 2248.00 2286.00 E 354141.00 1617309.00 555199.00 4175674.00 1800149.00 700291.00 1213866.00 988151.00 1947044.00 2116.00 2324.00 2191.00 F 293641.00 516673.00 549550.00 2823376.00 1667184.00 283627.00 567896.00 4461833.00 2913310.00 2229.00 2135.00 2361.00 G 170429.00 536909.00 167444.00 3107245.00 2588041.00 585431.00 7623400.00 881547.00 2014450.00 2210.00 2550.00 2153.00 H 228133.00 685799.00 387150.00 1288027.00 722284.00 236390.00 2858743.00 889615.00 2300603.00 2626.00 2191.00 2380.00