Load time: 10/08/2012 11:36:11 AM Read time: 10/08/2012 11:36:11 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2918 Comment: control+ein Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 72510096 cps Min counts: 11617 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 65615916.00 71813576.00 65639852.00 23991.00 72046272.00 72510096.00 65395756.00 25804.00 3467434.00 3707030.00 3575959.00 28524.00 B 55140356.00 57659132.00 60089196.00 25049.00 60457288.00 58897248.00 59605480.00 25351.00 1252207.00 9439387.00 998467.00 23670.00 C 35150092.00 32156496.00 35077908.00 25729.00 33831456.00 39841132.00 37716180.00 27467.00 3618870.00 14506996.00 18024994.00 23858.00 D 21304858.00 21597254.00 20632128.00 33738.00 20014086.00 22410686.00 21698942.00 26786.00 3578167.00 489495.00 4004205.00 24444.00 E 11571043.00 11056957.00 11987769.00 23991.00 13979829.00 14178825.00 10350080.00 24709.00 1547532.00 7950549.00 7022050.00 51968.00 F 7347244.00 7097176.00 6413734.00 24387.00 7686709.00 7716129.00 7916466.00 25332.00 1627497.00 2498223.00 4881034.00 24879.00 G 4112164.00 3778469.00 3678368.00 28562.00 3876012.00 3888467.00 4067077.00 26239.00 2565752.00 4511477.00 492669.00 23235.00 H 2145522.00 2176410.00 2280072.00 24501.00 2427191.00 2400416.00 2346150.00 24765.00 4624572.00 1224098.00 3929370.00 36742.00 Load time: 10/08/2012 11:38:13 AM Read time: 10/08/2012 11:38:13 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2919 Comment: setc Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.5 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 45423792 cps Min counts: 14848 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9021744.00 11799927.00 17686770.00 17987952.00 24336976.00 34864224.00 29629388.00 23884214.00 6465732.00 18960446.00 6908847.00 26340584.00 B 20880336.00 4885574.00 24377846.00 20209750.00 11644879.00 16533846.00 23807860.00 16927050.00 21533180.00 45423792.00 6054888.00 30808414.00 C 3398374.00 19714146.00 12941987.00 12305326.00 17924158.00 31747880.00 27764774.00 10478609.00 26873008.00 17943734.00 17996486.00 29903.00 D 11760757.00 6434980.00 27737304.00 17633926.00 14609659.00 20473972.00 17741640.00 15578412.00 11907704.00 16470111.00 19981264.00 31112.00 E 8252746.00 19225252.00 23818194.00 15126347.00 11847482.00 22042004.00 16448859.00 17203324.00 16396923.00 16203367.00 23668978.00 31944.00 F 9067108.00 8078068.00 22087750.00 12553133.00 15649651.00 31474108.00 18564004.00 21840856.00 8846377.00 5315321.00 29944604.00 33814.00 G 23721586.00 12446116.00 16493282.00 379683.00 20659010.00 4107102.00 652352.00 22049604.00 2669588.00 22189920.00 13212549.00 32813.00 H 8635559.00 9310986.00 15236795.00 17195868.00 9846311.00 32531534.00 22407246.00 29455910.00 16442007.00 21739116.00 24046694.00 29696.00