Load time: 10/22/2012 02:19:06 PM Read time: 10/22/2012 02:19:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2921 Comment: controls Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 64858412 cps Min counts: 9049 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 62170548.00 55323508.00 62535824.00 26920.00 64858412.00 45050580.00 59600960.00 26466.00 64384056.00 58265372.00 51964168.00 26353.00 B 48066172.00 49695152.00 51457232.00 24936.00 48250200.00 40302992.00 49454404.00 25050.00 40639336.00 49752028.00 34931076.00 19892.00 C 33308364.00 31000712.00 32452868.00 29999.00 32356658.00 26658444.00 39824464.00 29281.00 30239884.00 27452522.00 45235992.00 25900.00 D 19726038.00 20022650.00 17730228.00 26580.00 18981574.00 13625207.00 17866428.00 28922.00 17570000.00 19478994.00 12403574.00 26693.00 E 11335405.00 11902406.00 11007252.00 25541.00 11049911.00 9382269.00 11946729.00 26013.00 9807576.00 8559886.00 9112542.00 18929.00 F 6741357.00 6172721.00 5798380.00 22858.00 6773566.00 6466323.00 7132833.00 24011.00 6816967.00 4657561.00 5471593.00 19647.00 G 2857645.00 2905710.00 2751764.00 24917.00 3214145.00 3393784.00 3196793.00 26145.00 2963475.00 2648069.00 2546461.00 18098.00 H 1650909.00 1483110.00 1533890.00 22575.00 1781359.00 1904285.00 1507329.00 23670.00 1810025.00 1624059.00 1570804.00 19099.00 Load time: 10/22/2012 02:21:13 PM Read time: 10/22/2012 02:21:13 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2922 Comment: plate-2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10350656 cps Min counts: 8973 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 3721054.00 573166.00 2170800.00 2930906.00 2371024.00 4605835.00 1894652.00 3620114.00 879132.00 2099824.00 2235361.00 4028161.00 B 562133.00 1838279.00 3831683.00 2977297.00 4926730.00 2119278.00 2941641.00 3956198.00 1937175.00 4661293.00 9376778.00 10350656.00 C 1711985.00 2470952.00 5767582.00 1848327.00 3578893.00 1871141.00 2129671.00 3869262.00 4935155.00 1479256.00 3071643.00 18721.00 D 2955775.00 1653207.00 5383329.00 4245549.00 628253.00 1112743.00 2631257.00 2915968.00 2196325.00 1778644.00 404975.00 21838.00 E 1243472.00 838666.00 1846320.00 940151.00 1841515.00 1624563.00 1768021.00 1190349.00 669833.00 609796.00 531227.00 21063.00 F 231684.00 460875.00 1935281.00 1635094.00 1241186.00 1813416.00 6680566.00 9502196.00 1370592.00 280367.00 312105.00 17946.00 G 214361.00 67744.00 728113.00 292175.00 1499016.00 522896.00 1286110.00 3374456.00 764442.00 2756065.00 1566221.00 18230.00 H 263970.00 190142.00 1075300.00 1038254.00 707031.00 241300.00 1950628.00 3537791.00 520837.00 69841.00 640419.00 18645.00 Load time: 10/22/2012 02:23:21 PM Read time: 10/22/2012 02:23:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2923 Comment: plate-2 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 10989275 cps Min counts: 9766 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 6967570.00 3805977.00 4767587.00 10989275.00 3859275.00 2743954.00 1471529.00 1007442.00 1677313.00 733497.00 657044.00 365908.00 B 1194638.00 2592230.00 3613882.00 19533.00 2621627.00 1133448.00 3296818.00 1782273.00 659915.00 1215418.00 1011428.00 562379.00 C 3216379.00 5729107.00 2606210.00 1539765.00 3174407.00 459080.00 1175463.00 494143.00 557977.00 144746.00 1143593.00 20912.00 D 69520.00 5505661.00 2325608.00 3472969.00 597479.00 261287.00 1747065.00 1664616.00 56976.00 168606.00 240771.00 21914.00 E 1528166.00 3541850.00 951070.00 1162919.00 1153190.00 224638.00 1575446.00 909112.00 512222.00 756583.00 1114425.00 20402.00 F 2909356.00 5313368.00 3499215.00 2940055.00 687289.00 327445.00 794989.00 660558.00 1223409.00 473853.00 1156609.00 28148.00 G 4432141.00 4201560.00 831884.00 398741.00 1210299.00 277609.00 800071.00 904786.00 608115.00 163694.00 1572340.00 48796.00 H 3016645.00 1569106.00 3274008.00 1662598.00 1272036.00 3255294.00 3423150.00 2945018.00 910302.00 467865.00 3568395.00 49571.00