Load time: 11/20/2012 11:03:30 AM Read time: 11/20/2012 11:03:30 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: DNATech Fluorescence Int Plate ID: #2928 Comment: Standards,ein1_16 Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 22.6 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f1 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 4 A/D converter gain: x1 Integrating gain: x1 Max cps: 98078288 cps Min counts: 314 counts Well list: r0(a1:h12) = (f1(a1:h12)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9381400800.00 9807828800.00 8696110400.00 4975500.00 278191400.00 144843900.00 360100.00 368900.00 339600.00 348700.00 349700.00 388900.00 B 6742882400.00 6960394400.00 6346242000.00 5730200.00 159359100.00 24436900.00 366000.00 363900.00 357700.00 367400.00 379900.00 376100.00 C 3797970800.00 4071174400.00 4085627600.00 6298000.00 799156400.00 129127600.00 328000.00 369500.00 314800.00 339200.00 332000.00 368900.00 D 2568716800.00 2574463400.00 2371123400.00 5836700.00 48658900.00 37089400.00 331200.00 331800.00 412700.00 396300.00 361100.00 364700.00 E 1357057900.00 1448135800.00 1278650300.00 7112700.00 58790500.00 93610500.00 325700.00 370000.00 347400.00 346400.00 377300.00 389800.00 F 638379400.00 824147900.00 776159000.00 7460400.00 96499200.00 12991600.00 367900.00 361500.00 381600.00 394400.00 374000.00 353100.00 G 360823100.00 374848500.00 448590900.00 8162000.00 114505900.00 25971400.00 376700.00 394800.00 324200.00 369800.00 425600.00 390000.00 H 254805200.00 253625900.00 218919100.00 8162000.00 247321100.00 21717800.00 349700.00 355000.00 338100.00 333900.00 378800.00 366400.00 Load time: 11/20/2012 11:04:50 AM Read time: 11/20/2012 11:04:50 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: TILLING SYBR Green I Plate ID: #2929 Comment: standards,ein1-16 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 84995592 cps Min counts: 1029 counts Well list: r0(a1:h12) = cps(f2(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 84995592.00 72555504.00 78075088.00 34530.00 2779275.00 1470544.00 2285.00 2210.00 2436.00 2191.00 2417.00 2342.00 B 63015180.00 68918912.00 40787992.00 40537.00 1470449.00 244738.00 2191.00 2059.00 2266.00 2153.00 2342.00 2266.00 C 32659460.00 33888344.00 33701768.00 44958.00 7018384.00 1255954.00 2361.00 2096.00 2531.00 2493.00 2323.00 2266.00 D 21781152.00 20237756.00 20058326.00 55952.00 450581.00 341303.00 2172.00 2380.00 2323.00 2285.00 2474.00 2304.00 E 12859183.00 11014699.00 11404995.00 63829.00 575652.00 1020415.00 2153.00 2247.00 2229.00 2342.00 2210.00 2399.00 F 6646524.00 7162983.00 5713913.00 55083.00 1016165.00 117287.00 2323.00 2210.00 2247.00 3305.00 2399.00 2436.00 G 3242557.00 3152489.00 3539835.00 68192.00 1213150.00 276000.00 2266.00 2210.00 2304.00 2153.00 2512.00 2247.00 H 2023037.00 2474294.00 2387739.00 70780.00 2567452.00 221786.00 2323.00 2153.00 2361.00 2285.00 2323.00 2436.00