Load time: 10/24/2008 02:15:14 PM Read time: 10/24/2008 02:15:14 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: DNATech Fluorescence Int Plate ID: #1723 Comment: new comment Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 32.1 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f1 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 4 A/D converter gain: x1 Integrating gain: x1 Max cps: 175773136 cps Min counts: 1839 counts Well list: r0(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a6,a12,b6) = (f1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a6,a12,b6)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17577313600.00 157399100.00 632834900.00 178182200.00 91115100.00 4068200.00 11798470400.00 8879261600.00 17224300.00 189805100.00 27032000.00 1839900.00 B 13301750400.00 24542100.00 2026561000.00 23288100.00 98362700.00 5204000.00 4206536800.00 4872918800.00 4978545600.00 2939550600.00 7118085600.00 3573810800.00 C 452119900.00 3761084400.00 7919893600.00 5103931200.00 422821900.00 7361439200.00 4372022000.00 1653144700.00 5349617200.00 2334115000.00 731765800.00 4758499200.00 D 6890306400.00 4758499200.00 4372022000.00 9236336000.00 3462465600.00 9261165600.00 4468422400.00 960187700.00 5780458800.00 5219929600.00 3065083000.00 526138300.00 E 5939919600.00 7901728000.00 9261165600.00 4557081200.00 10162694400.00 6876553600.00 2385840200.00 4456861200.00 7622020800.00 8527607200.00 540585800.00 3696516400.00 F 2789598200.00 6904115200.00 6890306400.00 211449900.00 1279774700.00 8527607200.00 2617897800.00 6676654000.00 6917978400.00 1628144400.00 5939919600.00 3761084400.00 G 378921400.00 4866036000.00 3634128000.00 270505100.00 1169037500.00 5780458800.00 347188700.00 6475852400.00 4866036000.00 2980236400.00 44451300.00 4462634000.00 H 3978237200.00 331137400.00 5485913200.00 7622020800.00 6676654000.00 9261165600.00 7132823200.00 3250144800.00 4992976000.00 5503440000.00 3065083000.00 4758499200.00 Well list: r1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) = (f1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) - avg(f1(a6,a12,b6))) Data: BACKGROUND SUBTRACTED DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 17573609566.67 153695066.67 629130866.67 174478166.67 87411066.67 11794766366.67 8875557566.67 13520266.67 186101066.67 23327966.67 B 13298046366.67 20838066.67 2022856966.67 19584066.67 94658666.67 4202832766.67 4869214766.67 4974841566.67 2935846566.67 7114381566.67 3570106766.67 C 448415866.67 3757380366.67 7916189566.67 5100227166.67 419117866.67 7357735166.67 4368317966.67 1649440666.67 5345913166.67 2330410966.67 728061766.67 4754795166.67 D 6886602366.67 4754795166.67 4368317966.67 9232631966.67 3458761566.67 9257461566.67 4464718366.67 956483666.67 5776754766.67 5216225566.67 3061378966.67 522434266.67 E 5936215566.67 7898023966.67 9257461566.67 4553377166.67 10158990366.67 6872849566.67 2382136166.67 4453157166.67 7618316766.67 8523903166.67 536881766.67 3692812366.67 F 2785894166.67 6900411166.67 6886602366.67 207745866.67 1276070666.67 8523903166.67 2614193766.67 6672949966.67 6914274366.67 1624440366.67 5936215566.67 3757380366.67 G 375217366.67 4862331966.67 3630423966.67 266801066.67 1165333466.67 5776754766.67 343484666.67 6472148366.67 4862331966.67 2976532366.67 40747266.67 4458929966.67 H 3974533166.67 327433366.67 5482209166.67 7618316766.67 6672949966.67 9257461566.67 7129119166.67 3246440766.67 4989271966.67 5499735966.67 3061378966.67 4754795166.67 Load time: 10/24/2008 02:50:42 PM Read time: 10/24/2008 02:50:42 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: DNATech Fluorescence Int Plate ID: #1724 Comment: new comment Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 32.2 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f1 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 3 mm Numeric Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 4 A/D converter gain: x1 Integrating gain: x1 Max cps: 162507248 cps Min counts: 320 counts Well list: r0(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a6,a12,b6) = (f1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a6,a12,b6)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16250724800.00 5629335600.00 586309300.00 5310500.00 5016700.00 7148900.00 15176888000.00 4978545600.00 705395900.00 93567400.00 3642600.00 5574100.00 B 13250590400.00 70317800.00 10771100.00 4225600.00 12888100.00 6481100.00 3250144800.00 523659100.00 2944575600.00 9236336000.00 5790173600.00 307960400.00 C 5780458800.00 3201815400.00 4290353200.00 6475852400.00 5950178400.00 5939919600.00 6286776400.00 1064962500.00 4377577200.00 6500289600.00 6298269200.00 6890306400.00 D 289703400.00 7118085600.00 4978545600.00 3109344400.00 651997200.00 5227850400.00 479292300.00 6917978400.00 3577522000.00 313309700.00 1628144400.00 8222323200.00 E 2683141200.00 3973648800.00 9677397600.00 1970911400.00 6475852400.00 6676654000.00 2559549400.00 8202746400.00 5629335600.00 237138800.00 51337400.00 8527607200.00 F 6097616400.00 4201406800.00 5629335600.00 8879261600.00 931374300.00 3969070800.00 6286776400.00 7132823200.00 8222323200.00 5227850400.00 11185563200.00 6286776400.00 G 349800.00 326300.00 363700.00 399700.00 387700.00 378000.00 433500.00 377000.00 350500.00 320800.00 361400.00 404100.00 H 369800.00 359100.00 372600.00 390400.00 375900.00 374900.00 320800.00 388500.00 372100.00 330500.00 381800.00 361000.00 Well list: r1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) = (f1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) - avg(f1(a6,a12,b6))) Data: BACKGROUND SUBTRACTED DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 16244323433.33 5622934233.33 579907933.33 -1090866.67 -1384666.67 15170486633.33 4972144233.33 698994533.33 87166033.33 -2758766.67 B 13244189033.33 63916433.33 4369733.33 -2175766.67 6486733.33 3243743433.33 517257733.33 2938174233.33 9229934633.33 5783772233.33 301559033.33 C 5774057433.33 3195414033.33 4283951833.33 6469451033.33 5943777033.33 5933518233.33 6280375033.33 1058561133.33 4371175833.33 6493888233.33 6291867833.33 6883905033.33 D 283302033.33 7111684233.33 4972144233.33 3102943033.33 645595833.33 5221449033.33 472890933.33 6911577033.33 3571120633.33 306908333.33 1621743033.33 8215921833.33 E 2676739833.33 3967247433.33 9670996233.33 1964510033.33 6469451033.33 6670252633.33 2553148033.33 8196345033.33 5622934233.33 230737433.33 44936033.33 8521205833.33 F 6091215033.33 4195005433.33 5622934233.33 8872860233.33 924972933.33 3962669433.33 6280375033.33 7126421833.33 8215921833.33 5221449033.33 11179161833.33 6280375033.33 G -6051566.67 -6075066.67 -6037666.67 -6001666.67 -6013666.67 -6023366.67 -5967866.67 -6024366.67 -6050866.67 -6080566.67 -6039966.67 -5997266.67 H -6031566.67 -6042266.67 -6028766.67 -6010966.67 -6025466.67 -6026466.67 -6080566.67 -6012866.67 -6029266.67 -6070866.67 -6019566.67 -6040366.67