Load time: 10/24/2008 03:40:11 PM Read time: 10/24/2008 03:40:11 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: DNATech Fluorescence Int Plate ID: #1728 Comment: new comment Microplate format: MJRBlackSkirt96 Shake time: 0 s Temperature: 32.4 C Instrument tag: Serial number: AD2123 Read sequence: row Mode sequence: plate Detection mode: f1 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 1 Time between readings: Integration time: 100000 us Attenuator mode: m Motion settling time: 25 ms Z Height: 0 mm Bottom Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 4 A/D converter gain: x1 Integrating gain: x1 Max cps: 133533080 cps Min counts: 1901 counts Well list: r0(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a6,a12,b6) = (f1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12,a6,a12,b6)) Data: RAW DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13353308000.00 4866036000.00 10858900.00 70366600.00 12897400.00 5771200.00 10162694400.00 81297700.00 11906700.00 65867800.00 1901900.00 4615000.00 B 943360700.00 55111800.00 670263300.00 80428400.00 11616800.00 4625500.00 152144200.00 1705521600.00 3573810800.00 3515462800.00 242633500.00 3696516400.00 C 2334115000.00 3761084400.00 2412572600.00 1732974600.00 4201406800.00 2126638000.00 4866036000.00 2496488000.00 1492700900.00 4285016800.00 2240021800.00 5341322800.00 D 2904851200.00 599574200.00 3906069600.00 1869318200.00 271186500.00 3114967000.00 3515462800.00 4043607200.00 4462634000.00 3154902600.00 3404301600.00 3969070800.00 E 2309084000.00 578434000.00 3458989200.00 2787341000.00 1568831300.00 273251300.00 1918237000.00 4043607200.00 2586451600.00 119814700.00 907574700.00 3458989200.00 F 2980236400.00 2683141200.00 4557081200.00 5219929600.00 370446600.00 1692950000.00 178774000.00 1310941200.00 1513023000.00 156711800.00 724228200.00 4557081200.00 G 1335330700.00 3522651600.00 4758499200.00 1108479200.00 3109344400.00 2899961000.00 2359694200.00 161026000.00 1775852200.00 4285016800.00 3573810800.00 3065083000.00 H 173681800.00 2525772400.00 2555751600.00 1433688500.00 329112800.00 2439910400.00 1665934900.00 223508000.00 1868304400.00 6286776400.00 2619888800.00 154511900.00 Well list: r1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) = (f1(a1:a5,a7:a11,b1:b5,b7:b12,c1:c12,d1:d12,e1:e12,f1:f12,g1:g12,h1:h12) - avg(f1(a6,a12,b6))) Data: BACKGROUND SUBTRACTED DATA Units: rfu Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 13348304100.00 4861032100.00 5855000.00 65362700.00 7893500.00 10157690500.00 76293800.00 6902800.00 60863900.00 -3102000.00 B 938356800.00 50107900.00 665259400.00 75424500.00 6612900.00 147140300.00 1700517700.00 3568806900.00 3510458900.00 237629600.00 3691512500.00 C 2329111100.00 3756080500.00 2407568700.00 1727970700.00 4196402900.00 2121634100.00 4861032100.00 2491484100.00 1487697000.00 4280012900.00 2235017900.00 5336318900.00 D 2899847300.00 594570300.00 3901065700.00 1864314300.00 266182600.00 3109963100.00 3510458900.00 4038603300.00 4457630100.00 3149898700.00 3399297700.00 3964066900.00 E 2304080100.00 573430100.00 3453985300.00 2782337100.00 1563827400.00 268247400.00 1913233100.00 4038603300.00 2581447700.00 114810800.00 902570800.00 3453985300.00 F 2975232500.00 2678137300.00 4552077300.00 5214925700.00 365442700.00 1687946100.00 173770100.00 1305937300.00 1508019100.00 151707900.00 719224300.00 4552077300.00 G 1330326800.00 3517647700.00 4753495300.00 1103475300.00 3104340500.00 2894957100.00 2354690300.00 156022100.00 1770848300.00 4280012900.00 3568806900.00 3060079100.00 H 168677900.00 2520768500.00 2550747700.00 1428684600.00 324108900.00 2434906500.00 1660931000.00 218504100.00 1863300500.00 6281772500.00 2614884900.00 149508000.00