Load time: 12/02/2011 02:28:48 PM Read time: 12/02/2011 02:28:48 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Sybr Greeen 1 RWM Plate ID: #2034 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 86799464 cps Min counts: 1258505 counts Well list: r0(a1:h12) = cps(f6(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 86799464.00 73688056.00 7585772.00 6851265.00 4277265.00 7511367.00 9706274.00 6573694.00 5613254.00 7929690.00 16551876.00 13991210.00 B 77908088.00 54666956.00 15272335.00 20020580.00 6044441.00 15141226.00 19732796.00 19483392.00 24086128.00 17543356.00 30226066.00 20777710.00 C 51238312.00 27778476.00 13077373.00 23819632.00 15361354.00 13659341.00 9950425.00 10376641.00 18791034.00 9437843.00 12288221.00 29569248.00 D 21769758.00 21100340.00 10574356.00 10658963.00 16879474.00 13302298.00 24027702.00 20446480.00 21911380.00 14457774.00 12907566.00 12201766.00 E 19190822.00 12508081.00 13951242.00 8257701.00 9070971.00 12509287.00 22098656.00 18709046.00 17166428.00 15004847.00 23854340.00 18765002.00 F 6742772.00 5890195.00 31892600.00 32130596.00 15390307.00 19680792.00 23610060.00 13483378.00 16866902.00 14114377.00 26968820.00 13955837.00 G 3894797.00 3631608.00 23581238.00 24112758.00 17729220.00 20603994.00 15971701.00 18098782.00 15815025.00 11508813.00 18143716.00 7643330.00 H 2687796.00 2517011.00 21275922.00 13639166.00 20346074.00 25733686.00 24421152.00 29763010.00 30529106.00 16015695.00 13584465.00 14715909.00 Load time: 12/02/2011 02:33:02 PM Read time: 12/02/2011 02:33:02 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Sybr Greeen 1 RWM Plate ID: #2035 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 71007720 cps Min counts: 404086 counts Well list: r0(a1:h4,a5:f6,a7:h10) = cps(f6(a1:h4,a5:f6,a7:h10)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 71007720.00 47438148.00 19407586.00 15737467.00 13209759.00 1598457.00 26510518.00 21514418.00 3172196.00 2605550.00 B 50333160.00 50946980.00 13828936.00 12885351.00 16384057.00 2404563.00 46705712.00 53022792.00 8383307.00 6272394.00 C 31639578.00 24439360.00 16644348.00 18096886.00 8242509.00 808172.00 24631456.00 21903050.00 3784326.00 3016782.00 D 22795216.00 15472739.00 19088060.00 20556506.00 21453516.00 2839705.00 39746228.00 40764596.00 6250102.00 5563906.00 E 12029862.00 10872696.00 16487095.00 23632216.00 16064142.00 2530045.00 25837036.00 35243124.00 3480418.00 3996728.00 F 5655950.00 5882735.00 22633338.00 16003484.00 10085369.00 1429920.00 32306610.00 17166190.00 5123011.00 2151711.00 G 3352689.00 3408419.00 18110284.00 31727944.00 22492588.00 48699112.00 3464958.00 5689928.00 H 2352338.00 2314676.00 8194370.00 10177573.00 33428952.00 38359588.00 3782258.00 5411188.00