Load time: 12/05/2011 03:23:47 PM Read time: 12/05/2011 03:23:47 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Sybr Greeen 1 RWM Plate ID: #2040 Comment: Plate_3 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 52505064 cps Min counts: 43650 counts Well list: r0(a1:h12) = cps(f6(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 52505064.00 44954356.00 11022783.00 20377774.00 15190745.00 17953174.00 13796881.00 17782772.00 8126655.00 15785402.00 8697711.00 11189492.00 B 45073760.00 28555980.00 24035830.00 10267631.00 15818581.00 4660722.00 13139821.00 16144688.00 22198490.00 14163822.00 10786606.00 15660398.00 C 27438106.00 15610298.00 9449094.00 9228173.00 14006593.00 20205500.00 23483992.00 12668286.00 24936544.00 9109122.00 8454011.00 11238313.00 D 25656718.00 8053872.00 25605214.00 18605386.00 37632628.00 33583200.00 15122755.00 17550610.00 26110550.00 19759102.00 15389274.00 25983232.00 E 6511201.00 5776116.00 26559392.00 12635894.00 15898475.00 8203484.00 11077683.00 14695677.00 10820578.00 26640432.00 12012291.00 17509620.00 F 3964836.00 4324467.00 17858782.00 23764886.00 24598480.00 19530912.00 21126180.00 7947031.00 13509983.00 13026062.00 19956032.00 11512713.00 G 5839052.00 5209068.00 14061900.00 30884014.00 9849116.00 12200803.00 21180102.00 6810953.00 17597508.00 13785816.00 24855906.00 17076860.00 H 87300.00 17888518.00 16973906.00 16631356.00 20613546.00 11984066.00 13737793.00 15194700.00 25212210.00 21105282.00 16314346.00 49222440.00 Load time: 12/05/2011 03:27:07 PM Read time: 12/05/2011 03:27:07 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Sybr Greeen 1 RWM Plate ID: #2041 Comment: Plate_4 Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 42006388 cps Min counts: 44 counts Well list: r0(a1:h12) = cps(f6(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35680464.00 42006388.00 16579840.00 7241340.00 13565463.00 1258.00 899.00 359.00 539.00 629.00 359.00 179.00 B 20673304.00 24515456.00 14929177.00 19531576.00 8908551.00 809.00 539.00 899.00 629.00 719.00 719.00 449.00 C 13381988.00 11720044.00 16070744.00 23851774.00 36542124.00 809.00 629.00 539.00 988.00 629.00 988.00 899.00 D 7260401.00 7708610.00 14433572.00 23787684.00 22763744.00 449.00 449.00 629.00 719.00 719.00 899.00 89.00 E 5555034.00 6091602.00 18152322.00 9047575.00 26753614.00 629.00 809.00 1258.00 629.00 539.00 269.00 629.00 F 3327122.00 3706891.00 4786772.00 17571572.00 11679339.00 719.00 809.00 449.00 629.00 449.00 719.00 179.00 G 5438063.00 4450338.00 7076090.00 17673336.00 17425780.00 899.00 809.00 719.00 449.00 719.00 809.00 719.00 H 35308128.00 22797946.00 3347980.00 1924472.00 539.00 449.00 719.00 359.00 449.00 629.00 539.00 449.00 Load time: 12/05/2011 03:30:06 PM Read time: 12/05/2011 03:30:06 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Sybr Greeen 1 RWM Plate ID: #2042 Comment: Plate_3_HS Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 53437544 cps Min counts: 46527 counts Well list: r0(a1:h12) = cps(f6(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 53437544.00 47837916.00 11615694.00 21312638.00 15673965.00 18317112.00 14313118.00 18585322.00 8284689.00 17209078.00 8899382.00 11478048.00 B 48333516.00 30539422.00 25281150.00 10591488.00 16782530.00 4727028.00 13602192.00 16949064.00 22547958.00 14683911.00 10882507.00 16135860.00 C 29117446.00 16668327.00 9876010.00 9576355.00 14385482.00 20864466.00 24751484.00 12781485.00 25391356.00 9298745.00 8609432.00 11638729.00 D 26623336.00 8441430.00 26633906.00 19122448.00 39547908.00 35093016.00 15811803.00 18051422.00 26981402.00 20096224.00 15611266.00 26639012.00 E 6793621.00 6076316.00 28006642.00 12788333.00 17011982.00 8519848.00 11300879.00 15323564.00 11023812.00 27839318.00 12193611.00 17609362.00 F 4190686.00 4431608.00 18848576.00 25033348.00 25514818.00 20414654.00 21886402.00 8238153.00 14030437.00 13324889.00 20888718.00 12039206.00 G 6472796.00 5426728.00 14943813.00 32045534.00 10270808.00 12514923.00 22444296.00 7027502.00 18321122.00 14434179.00 24998046.00 16844906.00 H 93055.00 18951998.00 17769072.00 16677437.00 20669010.00 12236100.00 14029375.00 15348315.00 26078994.00 22586964.00 16878372.00 51792276.00 Load time: 12/05/2011 03:32:21 PM Read time: 12/05/2011 03:32:21 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Sybr Greeen 1 RWM Plate ID: #2043 Comment: Plate_4_HS Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 42052188 cps Min counts: 1234 counts Well list: r0(a1:h5) = cps(f6(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 35679336.00 42052188.00 16795862.00 7258896.00 13572301.00 B 20925624.00 25076910.00 15057480.00 19855030.00 8927887.00 C 13211440.00 11927218.00 16248628.00 24091718.00 37813556.00 D 7294872.00 7715648.00 14389554.00 24177120.00 22704860.00 E 5630574.00 6059369.00 18295150.00 9062615.00 26988798.00 F 3306815.00 3683964.00 4781639.00 17600360.00 11631598.00 G 5231251.00 4362302.00 7130734.00 18069948.00 17202570.00 H 36395376.00 23496608.00 3354469.00 1924042.00 2469.00