Load time: 04/06/2012 03:15:08 PM Read time: 04/06/2012 03:15:08 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #2482 Comment: redo Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 22.9 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 99349208 cps Min counts: 772777 counts Well list: r0(a1:h12) = cps(f6(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 88230752.00 99349208.00 12359641.00 13660391.00 4731717.00 12042065.00 7325242.00 15112948.00 10972968.00 8884863.00 3773005.00 6052392.00 B 58725292.00 61411020.00 17110902.00 14740159.00 37969676.00 13169829.00 10701190.00 37270868.00 23976602.00 28089882.00 24051224.00 17842820.00 C 33868220.00 32179904.00 19629496.00 11790089.00 17575956.00 13429293.00 10170204.00 7208937.00 8766315.00 17737324.00 15327816.00 10689748.00 D 18472660.00 18407624.00 8448920.00 6006143.00 10017421.00 2900309.00 4668295.00 13779625.00 13575751.00 14068361.00 7903174.00 7424010.00 E 10223079.00 10236093.00 4375925.00 9502913.00 22790832.00 8584360.00 1846763.00 6719527.00 6705487.00 9782866.00 11453975.00 6775704.00 F 6197801.00 6095247.00 11943112.00 14454417.00 22694922.00 20667924.00 8875414.00 8132629.00 6396358.00 9431839.00 13306961.00 7836367.00 G 3724394.00 3553511.00 15265985.00 28033938.00 14203232.00 15346018.00 16643361.00 16088916.00 17615378.00 17813700.00 4615101.00 8823529.00 H 2694077.00 2318771.00 12791054.00 8930968.00 11879588.00 6820706.00 10564797.00 9006998.00 4965213.00 10575423.00 5705554.00 12508606.00 Load time: 04/06/2012 03:17:10 PM Read time: 04/06/2012 03:17:10 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #2483 Comment: redo Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 23 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 106226688 cps Min counts: 39459 counts Well list: r0(a1:h5) = cps(f6(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 96520024.00 106226688.00 15820506.00 15214200.00 789154.00 B 58362500.00 60775912.00 14864315.00 8242366.00 6034032.00 C 33954824.00 34024496.00 12952185.00 12264543.00 88767.00 D 18653650.00 18786794.00 4555538.00 7407848.00 83616.00 E 10344891.00 10648402.00 7618191.00 5648577.00 88409.00 F 6142264.00 5603185.00 7991457.00 7410840.00 87333.00 G 3615641.00 3718718.00 19718752.00 17367394.00 82315.00 H 2326076.00 2245478.00 11575931.00 40794692.00 78919.00