Load time: 02/08/2012 04:24:41 PM Read time: 02/08/2012 04:24:41 PM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: Sybr Greeen 1 RWM Plate ID: #2200 Comment: 4matesDNAset_chicParental_8progenies Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 31.2 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 1 A/D converter gain: x1 Integrating gain: x1 Max cps: 60322504 cps Min counts: 1715007 counts Well list: r0(a1:h5) = cps(f6(a1:h5)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 59166368.00 58261192.00 15194750.00 7414073.00 37839216.00 B 46699080.00 40840980.00 9746184.00 8454554.00 42674016.00 C 31496984.00 24309118.00 12576812.00 3430015.00 17362628.00 D 20699830.00 17982290.00 14397186.00 7945012.00 41047192.00 E 14366630.00 9124272.00 28142638.00 8408264.00 42737084.00 F 7669346.00 6688291.00 29569734.00 7797101.00 38951168.00 G 4845446.00 4044267.00 20069722.00 8636307.00 43960432.00 H 5025722.00 3604000.00 60322504.00 6152585.00 30668122.00