Load time: 6/25/2012 15:04 Read time: 6/25/2012 15:04 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #2701 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 50908384 cps Min counts: 1076 counts Well list: r0(a1:h12) = cps(f6(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 8927744 4296787 5862819 5196630 4726573 5531927 5066406 5276383 4683801 50908384 43177224 2266 B 9343012 3431317 4805044 7308307 4381210 2724852 5845884 6211328 5596875 30177114 31361514 2341 C 8497715 3995910 5381777 6485222 5648405 5326994 5855562 7340688 6860804 19970856 19151488 2247 D 9466645 4138938 4603296 4957838 4636792 6035893 6818592 6720319 7225544 12055148 10973945 2360 E 8509831 5327764 5103874 5093520 4717084 5639021 7259761 6520866 5963537 6596901 6550812 2474 F 8284442 4727110 6288973 4302662 5337457 7093629 5823270 7955751 5057644 4178414 3983086 2152 G 10128779 4487262 4409996 7267236 4582850 5437771 7491730 7348546 5585088 2538178 2184764 2379 H 8508548 4897689 97450 7202871 4975344 400360 6509146 7412328 97073 1378192 1407144 2379 Load time: 6/25/2012 15:08 Read time: 6/25/2012 15:08 Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: Method ID: Sybr Greeen 1 RWM Plate ID: #2702 Comment: Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 24.1 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f6 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 3 A/D converter gain: x1 Integrating gain: x1 Max cps: 53653116 cps Min counts: 1181 counts Well list: r0(a1:h12) = cps(f6(a1:h12)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 9662198 4619078 6603920 5391000 5054299 5940311 5467191 5682934 5131241 53653116 44090084 2472 B 9958699 3650686 5163484 7329552 4693085 2899792 6305688 6563784 5854830 29929788 32657174 2490 C 9112592 4279349 5651295 6496050 5708640 5629133 6125834 7748829 7204227 20340754 19863816 2465 D 10221450 4368319 4976196 5179961 4736207 6406762 7378675 7126165 7558468 12332565 11165783 2389 E 9060481 5471864 5551694 5468924 4708312 6049597 7519304 6633168 6500891 6782857 6713900 2447 F 8873116 5057712 6796389 4619831 5450748 7559188 6280791 8768922 5478586 4369594 4084036 2362 G 10864716 4875040 4823349 7152105 4724088 5822108 7798158 7896287 6067863 2630734 2231819 2408 H 8995692 5146711 98821 7923953 5238242 398424 6728355 7716739 93267 1433917 1436436 2457