##BLOCKS= 6 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Kinetic Luminescence Reduced FALSE 21 2400 120 1 0 1 9 96 0 1 7 1 2 3 4 5 6 7 8 9 10 11 12 -0.09026135899349233 -0.0437356382665351 -0.22116257997645789 -0.070395373402101141 -0.10369275092939838 -0.045970915039153432 -0.12733106741501948 -0.18258180702191776 -0.548667961371382 NoFit NoFit NoFit -0.016218717456589152 -0.088620740954641522 -0.0096164587816196072 -0.069091492199457746 -0.31052044778996529 -0.032777485536529052 -0.018026950727427042 -0.017288759446206604 -0.11255360525264045 NoFit NoFit NoFit -0.041698968927888186 -0.0068267663375659021 -0.0095192248725307453 -0.031960570994328787 -0.0025535801313372945 -0.072106833899083136 -0.28864076987574044 -0.037264695016841959 -0.083033839227458453 NoFit NoFit NoFit -0.0016005060120609512 -0.0020884295366966044 -0.076090810027680325 -0.037109894829120936 -0.032837749138664246 -0.018151714504349929 -0.073930287379506063 -0.0042495400207002839 -0.0508232618179851 NoFit NoFit NoFit -0.065518941578121118 -0.50460289266969893 -0.0097262903438767936 -0.014366753122955732 -0.19074905056152597 -0.059337282400581066 -0.032360063940711663 -0.094352795640631579 -0.27779635548139514 NoFit NoFit NoFit 0.00037943412171120128 0.0017508724514723546 -0.020993034823303339 -0.058764669270146383 -0.016534989313740062 -0.016370445412368852 -0.0364928976900326 -0.0073793454083167873 -0.32382987143107761 NoFit NoFit NoFit -0.013306435790394708 -0.021568575930698147 -0.01485579446274477 -0.012444598504484547 -0.067675170655542641 -0.02912086593629851 -0.02855599046965783 -0.001990055536841364 -0.051544670806894809 NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit ~End Plate: Plate2 1.3 PlateFormat Kinetic Luminescence Reduced FALSE 21 2400 120 1 0 1 12 96 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 -0.052262793464241161 -0.23365768006580454 -0.0077312038816743783 -0.11498865664944508 -0.011237996592971479 -0.056632216866784865 -0.10884277656745918 -0.37401127630583464 -0.024763933430453546 -0.0011268128477003024 -5.5452254708738534E-05 0.0018280409936430534 -0.028191730489144404 -0.010659314932905619 -0.019659870604821005 -0.01833681615036863 -0.0057231919793371382 -0.024171749257976809 -0.029988802421956149 -0.0454225524809472 -0.00419777220117557 5.317454668233728E-05 -0.0001293472490770251 0.0014065871967250114 -0.013405133075072528 -0.02915717315241995 -0.13863873883723674 -0.010910764542691683 -0.0464293679272404 -0.0915046974034068 -0.021590910997494141 -0.022204550475709 -0.010682884640111983 -6.54113796189675E-05 4.2849451989911864E-05 0.00017340478332672556 -0.041980020621304785 -0.064383299984965281 -0.012775959087339027 0.00016327874666758677 -0.18373430794022952 -0.0041536719625828063 -0.011956199565064845 -0.0029522866880007689 -0.0031989020803144144 -0.0020650947333289743 -0.00027143099336396012 0.00056248495425665409 -0.037317681090997284 -0.046236544728042256 -0.017228480162883395 -0.055541300259807448 -0.073513909051240472 -0.16225565378496237 -0.019947231090687748 -0.0073115570578734844 -0.15478740582277129 -0.0016443310729299598 -0.0015997514298025204 -0.000606138707140007 -0.08437758911575978 -0.0076544149920904064 -0.13046516113789791 -0.02173393522446402 -0.0028423011159594736 -0.22588622916484968 -0.15488581225167802 -0.027552037885228153 -0.065109430771564114 0.00088804342347781969 -0.0016348893787332456 0.0010492682843793717 -0.007584585236586886 -0.021211483639015774 0.0019913979815341606 -0.028638277358045302 -0.015334319455472751 0.00507328483991211 -0.082214448558439013 -0.013234321711478288 0.0065231031238215074 -0.0026301620771511181 -0.0011032303935694288 0.00092014043691213089 -0.066554457370668382 -0.033997547046031383 -0.010480014074667594 -0.012608643599981345 0.010306642939788089 -0.38703131067107532 -0.043220750930771538 -0.069833813746607423 -0.15856504402728355 -0.00227279719862315 -0.00074661316293706621 -0.0018732259225495789 ~End Plate: Plate3 1.3 PlateFormat Kinetic Luminescence Reduced FALSE 21 2400 120 1 0 1 9 96 0 1 6 1 2 3 4 5 6 7 8 9 10 11 12 -0.0024981044391206735 -0.0066407024828400579 -0.007846365515768991 -0.0030917397670717373 -0.0050510585419807179 -0.0027137979921015879 -0.0049744533300775341 -0.0050410789854933242 -0.0031379578981393752 NoFit NoFit NoFit -0.0037002622410306969 -0.0084066658272028121 -0.0018719574840281349 -0.00210948782969141 -0.0018153864922880248 -0.0018389567588764301 -0.0017628932200152863 -0.00099444797137213125 -0.0035691553906395342 NoFit NoFit NoFit -0.0035148358978127985 -0.0036882646616895874 -0.0024447611680682968 -0.0016875219548383239 -0.0033540070300089142 -0.0021535327879134954 -0.00425171501151922 -0.0063057514186785806 -0.0042616447826703784 NoFit NoFit NoFit -0.0062753860545500554 -0.0041330107613469043 -0.0024454812528713538 -0.0018284519241732423 -0.0043598795772529951 -0.0056795948069475492 -0.0032685595990412054 -0.0041538593580231651 -0.0046069147161368054 NoFit NoFit NoFit 0.0004223418399785326 -0.0015371902393452289 -0.0018717987288912584 -0.001828820979437367 -0.00069198282623453115 8.6035670466495622E-05 -0.0016551363790180218 -0.0023483895896947043 -0.0021418734913485021 NoFit NoFit NoFit -0.0023908130910173134 -0.00067207388486152631 -0.0010056166248647383 -0.0012534223205821393 -0.0014508362860738986 -0.0010850158670911842 -0.0016233397293907983 -0.0017086235125247924 -0.00046472096368245237 NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit NoFit ~End Plate: Plate4 1.3 PlateFormat Kinetic Luminescence Reduced FALSE 21 2400 120 1 0 1 9 96 0 1 8 1 2 3 4 5 6 7 8 9 10 11 12 -0.0032451507957694647 -0.0642578301488642 -0.0020666652176022557 -0.002024668988372046 -0.011587206238235458 -0.0030493238837373052 -0.0019798499722732642 -0.010773878089530432 0.0034945861951816448 NoFit NoFit NoFit -0.0005960681934844103 -0.0023051255588378296 -0.0015479706628121384 -0.002563345794413239 0.000671540790027884 7.6077093184052038E-05 -0.0026810768906143719 -0.0010814813134242727 -0.0014836930350442211 NoFit NoFit NoFit 0.0019918114093132214 -0.0055167034223354734 -0.0077030987595342881 0.0037901101180144521 -0.0018502054640048987 -0.002769807727214598 -0.0017625941371575546 -0.0021304229396332934 -0.0013619788274324902 NoFit NoFit NoFit -0.0020880718663687015 -0.0024458082143890589 -0.0015244820735391773 -0.0016663744948912222 -0.0023479725897295775 -0.00039934065227210209 -0.00082240139221114068 -9.7045079532084684E-05 -0.0020440749399504433 NoFit NoFit NoFit 0.0008105376789092403 -0.00204388361257922 -0.0002914128306637889 -0.00036697671328142462 -0.0011039354988611563 0.0034929653201639229 -0.0010704077589899395 -0.0019594060117670022 -0.00803719059806194 NoFit NoFit NoFit -0.00024851210965508563 -0.0010597311509119162 -0.0013079140384349569 -0.0014179906235011032 0.0010383309398093756 -0.00093036062961930025 -0.00099491069932362776 -0.0018502798833996289 -0.0012105824828591506 NoFit NoFit NoFit 0.00042064687348164465 0.0019023326449206406 -0.00023849716467310434 0.0010280366002139655 0.0032774193285810826 -0.0005078573913743327 0.0019252504709118814 0.0072698026490012014 -0.00062739000344232717 NoFit NoFit NoFit 0.0002593761825059875 -0.00085399023646779012 0.0011036092380545077 -0.0010280314500828417 0.011507007752620131 7.6994460290112486E-08 -0.00046534267269330464 -0.0016554423115562179 -0.00048702536848794966 NoFit NoFit NoFit ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 -0.090 R 02 B1 -0.016 R 03 C1 -0.042 R 04 D1 -0.002 R 05 E1 -0.066 R 06 F1 0.000 R 064 A1 -0.052 R 065 B1 -0.028 R 066 C1 -0.013 R 067 D1 -0.042 R 068 E1 -0.037 R 069 F1 -0.084 R 07 G1 -0.013 R 070 G1 -0.008 R 071 H1 -0.067 R 072 A2 -0.234 R 073 B2 -0.011 R 074 C2 -0.029 R 075 D2 -0.064 R 076 E2 -0.046 R 077 F2 -0.008 R 078 G2 -0.021 R 079 H2 -0.034 R 08 A2 -0.044 R 080 A3 -0.008 R 081 B3 -0.020 R 082 C3 -0.139 R 083 D3 -0.013 R 084 E3 -0.017 R 085 F3 -0.130 R 086 G3 0.002 R 087 H3 -0.010 R 088 A4 -0.115 R 089 B4 -0.018 R 09 B2 -0.089 R 090 C4 -0.011 R 091 D4 0.000 R 092 E4 -0.056 R 093 F4 -0.022 R 094 G4 -0.029 R 095 H4 -0.013 R 096 A5 -0.011 R 097 B5 -0.006 R 098 C5 -0.046 R 099 D5 -0.184 R 10 C2 -0.007 R 100 E5 -0.074 R 101 F5 -0.003 R 102 G5 -0.015 R 103 H5 0.010 R 104 A6 -0.057 R 105 B6 -0.024 R 106 C6 -0.092 R 107 D6 -0.004 R 108 E6 -0.162 R 109 F6 -0.226 R 11 D2 -0.002 R 110 G6 0.005 R 111 H6 -0.387 R 112 A7 -0.109 R 113 B7 -0.030 R 114 C7 -0.022 R 115 D7 -0.012 R 116 E7 -0.020 R 117 F7 -0.155 R 118 G7 -0.082 R 119 H7 -0.043 R 12 E2 -0.505 R 120 A8 -0.374 R 121 B8 -0.045 R 122 C8 -0.022 R 123 D8 -0.003 R 124 E8 -0.007 R 125 F8 -0.028 R 126 G8 -0.013 R 127 H8 -0.070 R 128 A9 -0.025 R 129 B9 -0.004 R 13 F2 0.002 R 130 C9 -0.011 R 131 D9 -0.003 R 132 E9 -0.155 R 133 F9 -0.065 R 134 G9 0.007 R 135 H9 -0.159 R 136 A1 -0.002 R 137 B1 -0.004 R 138 C1 -0.004 R 139 D1 -0.006 R 14 G2 -0.022 R 140 E1 0.000 R 141 F1 -0.002 R 142 A2 -0.007 R 143 B2 -0.008 R 144 C2 -0.004 R 145 D2 -0.004 R 146 E2 -0.002 R 147 F2 -0.001 R 148 A3 -0.008 R 149 B3 -0.002 R 15 A3 -0.221 R 150 C3 -0.002 R 151 D3 -0.002 R 152 E3 -0.002 R 153 F3 -0.001 R 154 A4 -0.003 R 155 B4 -0.002 R 156 C4 -0.002 R 157 D4 -0.002 R 158 E4 -0.002 R 159 F4 -0.001 R 16 B3 -0.010 R 160 A5 -0.005 R 161 B5 -0.002 R 162 C5 -0.003 R 163 D5 -0.004 R 164 E5 -0.001 R 165 F5 -0.001 R 166 A6 -0.003 R 167 B6 -0.002 R 168 C6 -0.002 R 169 D6 -0.006 R 17 C3 -0.010 R 170 E6 0.000 R 171 F6 -0.001 R 172 A7 -0.005 R 173 B7 -0.002 R 174 C7 -0.004 R 175 D7 -0.003 R 176 E7 -0.002 R 177 F7 -0.002 R 178 A8 -0.005 R 179 B8 -0.001 R 18 D3 -0.076 R 180 C8 -0.006 R 181 D8 -0.004 R 182 E8 -0.002 R 183 F8 -0.002 R 184 A9 -0.003 R 185 B9 -0.004 R 186 C9 -0.004 R 187 D9 -0.005 R 188 E9 -0.002 R 189 F9 -0.000 R 19 E3 -0.010 R 190 A1 -0.003 R 191 B1 -0.001 R 192 C1 0.002 R 193 D1 -0.002 R 194 E1 0.001 R 195 F1 -0.000 R 196 G1 0.000 R 197 H1 0.000 R 198 A2 -0.064 R 199 B2 -0.002 R 20 F3 -0.021 R 200 C2 -0.006 R 201 D2 -0.002 R 202 E2 -0.002 R 203 F2 -0.001 R 204 G2 0.002 R 205 H2 -0.001 R 206 A3 -0.002 R 207 B3 -0.002 R 208 C3 -0.008 R 209 D3 -0.002 R 21 G3 -0.015 R 210 E3 -0.000 R 211 F3 -0.001 R 212 G3 -0.000 R 213 H3 0.001 R 214 A4 -0.002 R 215 B4 -0.003 R 216 C4 0.004 R 217 D4 -0.002 R 218 E4 -0.000 R 219 F4 -0.001 R 22 A4 -0.070 R 220 G4 0.001 R 221 H4 -0.001 R 222 A5 -0.012 R 223 B5 0.001 R 224 C5 -0.002 R 225 D5 -0.002 R 226 E5 -0.001 R 227 F5 0.001 R 228 G5 0.003 R 229 H5 0.012 R 23 B4 -0.069 R 230 A6 -0.003 R 231 B6 0.000 R 232 C6 -0.003 R 233 D6 -0.000 R 234 E6 0.003 R 235 F6 -0.001 R 236 G6 -0.001 R 237 H6 0.000 R 238 A7 -0.002 R 239 B7 -0.003 R 24 C4 -0.032 R 240 C7 -0.002 R 241 D7 -0.001 R 242 E7 -0.001 R 243 F7 -0.001 R 244 G7 0.002 R 245 H7 -0.000 R 246 A8 -0.011 R 247 B8 -0.001 R 248 C8 -0.002 R 249 D8 -0.000 R 25 D4 -0.037 R 250 E8 -0.002 R 251 F8 -0.002 R 252 G8 0.007 R 253 H8 -0.002 R 254 A9 0.003 R 255 B9 -0.001 R 256 C9 -0.001 R 257 D9 -0.002 R 258 E9 -0.008 R 259 F9 -0.001 R 26 E4 -0.014 R 260 G9 -0.001 R 261 H9 -0.000 R 27 F4 -0.059 R 28 G4 -0.012 R 29 A5 -0.104 R 30 B5 -0.311 R 31 C5 -0.003 R 32 D5 -0.033 R 33 E5 -0.191 R 34 F5 -0.017 R 35 G5 -0.068 R 36 A6 -0.046 R 37 B6 -0.033 R 38 C6 -0.072 R 39 D6 -0.018 R 40 E6 -0.059 R 41 F6 -0.016 R 42 G6 -0.029 R 43 A7 -0.127 R 44 B7 -0.018 R 45 C7 -0.289 R 46 D7 -0.074 R 47 E7 -0.032 R 48 F7 -0.036 R 49 G7 -0.029 R 50 A8 -0.183 R 51 B8 -0.017 R 52 C8 -0.037 R 53 D8 -0.004 R 54 E8 -0.094 R 55 F8 -0.007 R 56 G8 -0.002 R 57 A9 -0.549 R 58 B9 -0.113 R 59 C9 -0.083 R 60 D9 -0.051 R 61 E9 -0.278 R 62 F9 -0.324 R 63 G9 -0.052 R Group Summaries InRange R - Outside standard range ~End Original Filename: 20181102_ROS_CG_Agroinfiltration_HTZ_reduced; Date Last Saved: 11/2/2018 5:54:57 PM