Load time: 03/12/2012 09:46:43 AM Read time: 03/12/2012 09:46:43 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2315 Comment: libraries test34 12hpi cb and test234 24hpi cb quiagen Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.7 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 68737632 cps Min counts: 582588 counts Well list: r0(a1:h2,a3,b3) = cps(f2(a1:h2,a3,b3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 68737632.00 2499605.00 1407323.00 B 36357160.00 2086919.00 2052256.00 C 22444132.00 1751553.00 D 13474666.00 1359216.00 E 7800050.00 2000136.00 F 5367078.00 1165176.00 G 3654305.00 1954336.00 H 1913734.00 1876544.00 Load time: 03/12/2012 09:47:31 AM Read time: 03/12/2012 09:47:31 AM Kinetic read cycle: 1 of 1 Read start delay time: 0 s Time delay between kinetic reads: Barcode: ERROR Method ID: TILLING SYBR Green I Plate ID: #2316 Comment: libraries test34 12hpi cb and test234 24hpi cb quiagen Microplate format: Dynatech Microfluor 96 Shake time: 0 s Temperature: 30.8 C Instrument tag: Serial number: AD2123 Read sequence: column Mode sequence: plate Detection mode: f2 Light sensor: HC-120 Excitation side: Top Emission side: Top Lamp: Continuous Readings per well: 5 Time between readings: 100 ms Integration time: 100000 us Attenuator mode: m Motion settling time: 250 ms Z Height: 4.995 mm Middle Excitation filter: 1 Fluorescein 485 nm Emission filter: 1 Fluorescein 530 nm Beamsplitter: Top Fluorescein 505 Excitation polarizer filter: o Emission polarizer filter: o Detector counting: SmartRead Flash lamp voltage: Delay after flash: Sensitivity setting: 2 A/D converter gain: x1 Integrating gain: x1 Max cps: 68779512 cps Min counts: 577397 counts Well list: r0(a1:h2,a3,b3) = cps(f2(a1:h2,a3,b3)) Data: RAW DATA Units: cps Display format: %.2f 1 2 3 4 5 6 7 8 9 10 11 12 A 68779512.00 2481889.00 1407037.00 B 36262528.00 2072036.00 2044974.00 C 22574504.00 1752394.00 D 13462065.00 1354330.00 E 7744223.00 1997223.00 F 5339972.00 1154794.00 G 3659430.00 1946287.00 H 1898573.00 1869682.00