##BLOCKS= 4 Note: Basic Endpoint Protocol Use this protocol for endpoint assays that have unknowns that will have concentrations interpolated from a standard curve. Modify the instrument setup for the wavelength(s) of interest for your assay. You may also modify the template to include additional standards, unknowns, and controls. To make modifications, click the plate section to make it active. READER SUITABILITY: SpectraMax M2, M2e, M3, M4, M5, and M5e. SpectraMax Plus 384, 190, SpectraMax 190, 340PC 384 and VersaMax Emax and Vmax PROTOCOL REVISION HISTORY: 03/02/11 - Imported from 5.4 and edited. (ELM) 10/11/11 - Updated with the additional instruments supported in SMP 6.1 ~End Plate: Plate1 1.3 PlateFormat Endpoint Absorbance Raw FALSE 1 1 1 12 96 1 8 Temperature(¡C) 1 2 3 4 5 6 7 8 9 10 11 12 24 0.0137 0.0133 0.0124 0.0129 0.0117 0.0053 0.0133 0.0131 0.0129 0.0134 0.016 0.0153 0.0146 0.0156 0.0139 0.0128 0.0131 0.0057 0.0142 0.0139 0.0128 0.0119 0.0131 0.0094 0.0145 0.014 0.014 0.0143 0.0104 0.0057 0.0165 0.0169 0.0158 0.0133 0.0148 0.0056 0.0153 0.017 0.0131 0.0141 0.0055 0.0054 0.0154 0.0142 0.0147 0.0153 0.0055 0 0.015 0.014 0.0139 0.0135 0.0058 0.0055 0.0144 0.0132 0.0138 0.0138 0.0055 0.0052 0.0161 0.0139 0.0146 0.0133 0.0062 0.0055 0.0141 0.014 0.0109 0.014 0.0055 0.0053 0.0058 0.0059 0.0056 0.0058 0.0056 0.0055 0.0056 0.0055 0.0055 0.0055 0.0055 0.0055 0.0055 0.0058 0.0055 0.0056 0.0056 0.0054 0.0053 0.0057 0.0058 0.0053 0.0055 0.0057 1 2 3 4 5 6 7 8 9 10 11 12 0.0137 0.013300000000000003 0.012400000000000005 0.012899999999999998 0.011699999999999999 0.0053000000000000026 0.013300000000000003 0.013100000000000004 0.012899999999999998 0.013399999999999999 0.016000000000000004 0.015300000000000005 0.014599999999999998 0.0156 0.0139 0.012800000000000002 0.013100000000000004 0.0057 0.0142 0.0139 0.012800000000000002 0.011900000000000004 0.013100000000000004 0.0094000000000000021 0.014500000000000003 0.014000000000000002 0.014000000000000002 0.014300000000000004 0.010400000000000003 0.0057 0.016500000000000004 0.016900000000000002 0.015800000000000005 0.013300000000000003 0.014800000000000004 0.0056000000000000008 0.015300000000000005 0.017000000000000005 0.013100000000000004 0.014100000000000005 0.0055000000000000014 0.005400000000000002 0.0154 0.0142 0.014700000000000001 0.015300000000000005 0.0055000000000000014 0 0.015000000000000003 0.014000000000000002 0.0139 0.013500000000000002 0.0058000000000000031 0.0055000000000000014 0.0144 0.0132 0.013800000000000003 0.013800000000000003 0.0055000000000000014 0.0052000000000000032 0.0161 0.0139 0.014599999999999998 0.013300000000000003 0.0062000000000000006 0.0055000000000000014 0.014100000000000005 0.014000000000000002 0.010900000000000003 0.014000000000000002 0.0055000000000000014 0.0053000000000000026 0.0058000000000000031 0.005899999999999999 0.0056000000000000008 0.0058000000000000031 0.0056000000000000008 0.0055000000000000014 0.0056000000000000008 0.0055000000000000014 0.0055000000000000014 0.0055000000000000014 0.0055000000000000014 0.0055000000000000014 0.0055000000000000014 0.0058000000000000031 0.0055000000000000014 0.0056000000000000008 0.0056000000000000008 0.005400000000000002 0.0053000000000000026 0.0057 0.0058000000000000031 0.0053000000000000026 0.0055000000000000014 0.0057 ~End Group: Standards Sample Conc BackCalcConc Wells Value MeanValue SD CV 01 1.000 0.990 A12 0.015 0.015 0.000 0.0 02 0.500 0.529 B12 0.009 0.009 0.000 0.0 03 0.250 0.231 C12 0.006 0.006 0.000 0.0 Group Summaries MinStd Smallest standard value: 0.006 Min(MeanValue) MaxStd Largest standard value: 0.015 Max(MeanValue) ~End Group: Unknowns Sample Wells Value R Result MeanResult SD CV 01 A1 0.014 0.865 0.865 0.000 0.0 02 A2 0.013 0.834 0.834 0.000 0.0 03 A3 0.012 0.763 0.763 0.000 0.0 04 A4 0.013 0.803 0.803 0.000 0.0 05 A5 0.012 0.709 0.709 0.000 0.0 06 B1 0.015 0.936 0.936 0.000 0.0 07 B2 0.016 R 1.014 1.014 0.000 0.0 08 B3 0.014 0.881 0.881 0.000 0.0 09 B4 0.013 0.795 0.795 0.000 0.0 10 B5 0.013 0.818 0.818 0.000 0.0 11 C1 0.015 0.928 0.928 0.000 0.0 12 C2 0.014 0.889 0.889 0.000 0.0 13 C3 0.014 0.889 0.889 0.000 0.0 14 C4 0.014 0.912 0.912 0.000 0.0 15 C5 0.010 0.607 0.607 0.000 0.0 16 D1 0.015 0.990 0.990 0.000 0.0 17 D2 0.017 R 1.124 1.124 0.000 0.0 18 D3 0.013 0.818 0.818 0.000 0.0 19 D4 0.014 0.897 0.897 0.000 0.0 20 E1 0.015 0.967 0.967 0.000 0.0 21 E2 0.014 0.889 0.889 0.000 0.0 22 E3 0.014 0.881 0.881 0.000 0.0 23 E4 0.014 0.850 0.850 0.000 0.0 24 F1 0.016 R 1.053 1.053 0.000 0.0 25 F2 0.014 0.881 0.881 0.000 0.0 26 F3 0.015 0.936 0.936 0.000 0.0 27 F4 0.013 0.834 0.834 0.000 0.0 28 A7 0.013 0.834 0.834 0.000 0.0 29 A8 0.013 0.818 0.818 0.000 0.0 30 A9 0.013 0.803 0.803 0.000 0.0 31 A10 0.013 0.842 0.842 0.000 0.0 32 A11 0.016 R 1.045 1.045 0.000 0.0 33 B7 0.014 0.904 0.904 0.000 0.0 34 B8 0.014 0.881 0.881 0.000 0.0 35 B9 0.013 0.795 0.795 0.000 0.0 36 B10 0.012 0.724 0.724 0.000 0.0 37 B11 0.013 0.818 0.818 0.000 0.0 38 C7 0.017 R 1.084 1.084 0.000 0.0 39 C8 0.017 R 1.116 1.116 0.000 0.0 40 C9 0.016 R 1.030 1.030 0.000 0.0 41 C10 0.013 0.834 0.834 0.000 0.0 42 C11 0.015 0.951 0.951 0.000 0.0 43 D7 0.015 R 0.998 0.998 0.000 0.0 44 D8 0.014 0.904 0.904 0.000 0.0 45 D9 0.015 0.944 0.944 0.000 0.0 46 D10 0.015 0.990 0.990 0.000 0.0 47 E7 0.014 0.920 0.920 0.000 0.0 48 E8 0.013 0.826 0.826 0.000 0.0 49 E9 0.014 0.873 0.873 0.000 0.0 50 E10 0.014 0.873 0.873 0.000 0.0 51 F7 0.014 0.897 0.897 0.000 0.0 52 F8 0.014 0.889 0.889 0.000 0.0 53 F9 0.011 0.646 0.646 0.000 0.0 54 F10 0.014 0.889 0.889 0.000 0.0 Group Summaries InRange R - Outside standard range ~End Original Filename: 20160816_plateresults; Date Last Saved: 8/16/2016 3:08:24 PM